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Multiplex RT-PCR Assay for the Detection of Apple stem grooving virus andApple chlorotic Leaf spot virus in Infected Korean Apple Cultivars
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2006, 22권 2호 12 168-173 (6 pages)
To develop the diagnostic method for the viral infection in apple, the partial genes corresponding to the Nterminal region of RNA polymerase of Apple stem grooving virus (ASGV) and coat protein of Apple chlorotic leaf spot virus (ACLSV) were characterized from the infected apple cultivars in Korea. Based on the nucleotide sequences of the characterized partial genes, the virus gene-specific primers were designed for the detection of ASGV and ACLSV infected in species of Malus. The RT-PCR using... -
Rapid and Visual Detection of Barley Yellow Dwarf Virus by Reverse Transcription Recombinase Polymerase Amplification with Lateral Flow Strips
Na-Kyeong Kim, Hyo-Jeong Lee, Sang-Min Kim, Rae-Dong Jeong 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2022, 38권 2호 8 159-166 (8 pages)
Barley yellow dwarf virus (BYDV) has been a major viral pathogen causing significant losses of cereal crops including oats worldwide. It spreads naturally through aphids, and a rapid, specific, and reliable diagnostic method is imperative for disease monitoring and management. Here, we established a rapid and reliable method for isothermal reverse transcription recombinase polymerase amplification (RT-RPA) combined with a lateral flow strips (LFS) assay for the detection of BYDV-infected oat... -
Identification of Plant Viruses Infecting Pear Using RNA Sequencing
Nam-Yeon Kim, Hyo-Jeong Lee, Hong-Sup Kim, Su-Heon Lee, Jae-Sun Moon, Rae-Dong Jeong 한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 3호 5 258-267 (10 pages)
110) of pear trees from 35 orchards in five major pear-producing regions were collected and subjected to RNA sequencing. Most virus-associated contigs matched the sequences of known viruses, including apple stem grooving virus (ASGV) and apple stem pit- ting virus (ASPV). However, some contigs matched the sequences of apple green crinkle-associated virus and cucumber mosaic virus. In addition, three complete or nearly complete genomes were constructed based on transcriptome data and subjected to... -
Genetic Diversity of a Natural Population of Apple stem pitting virus Isolated from Apple in Korea
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2014, 30권 2호 11 195-199 (5 pages)
Apple stem pitting virus (ASPV), of the Foveavirus genus in the family Betaflexiviridae, is one of the most common viruses of apple and pear trees. To examine variability of the coat protein (CP) gene from ASPV, eight isolates originating from 251 apple trees, which were collected from 22 apple orchards located in intensive apple growing areas of the North Gyeongsang and North Jeolla Provinces in Korea, were sequenced and compared. The nucleotide sequence identity of the CP gene of eight ASPV... -
Microwave Irradiation-assisted RNA Extraction from Woody Tissues for Plant Virus Detection
한국식물병리학회 The Plant Pathology Journal 3 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 3호 14 286-288 (3 pages)
Plant tissue homogenization using a mortar or mechanical equipment has been the preferred method for obtaining high yields of total RNA; this method, however, is both time-consuming and expensive. Additionally, homogenization may generate excessive endogenous RNases, polyphenolics, and other substances that reduce the quality and quantity of RNA. In this study, we describe the microwave irradiation-assisted RNA extraction (MIRE) technique which, without tissue disruption and homogenization,... -
저온과 바이러스 감염에 노출된 사과나무의 생리적 유전자 정량 측정용 유전자들의 발현 분석 및 검증
윤주연, 정재훈, 최승국 한국식물병리학회 식물병연구 15 Pages
한국식물병리학회 식물병연구 2020, 26권 3호 3 144-158 (15 pages)
사과 식물에서 유전자 발현 변화의 정량적변화를 분석하기 위해, 사과 잎검은점 바이러스(Apple stem grooving virus, ASGV)에 의한 감염 동안 발현의 안정성에 대해 10개 참조유전자들(ACT, CKL, EF-1α, GAPDH, MDH, PDI, THF, UBC, UBC10 및 WD40)을 평가하였다. AGSV 감염 또는저온 처리된 사과 식물에서의 10개 참조유전자 발현의 안정은 5가지 프로그램을 사용하여 분석하였다. ASGV 감염 사과식물의 잎 조직에서는 CKL>THFs>GAPDH>ACT 순서로 가장안정한 유전자로 분석되었으며 WD40CKL>UBC10이고 가장 안정하지 않은 유전자는 ACT -
건식그루빙을 사용한 공항 활주로의 마찰 및 수막현상 특성평가
박태순, Park. Tae-Soon 한국도로학회 한국도로포장공학회 논문집 8 Pages
한국도로학회 한국도로포장공학회 논문집 2001, Vol.3 No.3 111-118 (8 pages)
초대형 점보 항공기의 출현으로 인하여 활주로 표면의 마찰저항 성능이 중요하게 되었다. 우천시나 겨울철과 같은 특정한 기후에서 활주로 표면은 수막현상과 마찰력의 손실이 발생하여 항공기의 브레이크 기능이 저하되어 제동력을 잃게 된다. 이러한 문제를 해결하기 위해 많은 연구가 미국항공우주국, 미공군, 미연방항공청 등에 의해 수행되었다. 그 결과 다양한 종류의 마찰저항이 끈 포장표면 처리 방법이 개발되었다. 이러한 설계방법 가운데 대표적인 방법이 그루빙이다. 포장 그루빙 공법은 활주로 표면에 마찰저항을 증가시키고... -
ERW 강관 용접부의 홈부식거동에 미치는 입열량의 영향
이병우, 이재식, 박화순, Lee. B.W., Lee. J.S., Park. H.S. 한국동력기계공학회 한국동력기계공학회지 6 Pages
한국동력기계공학회 한국동력기계공학회지 2007, Vol.11 No.3 41-46 (6 pages)
