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Comparison of PCR method and bacterial culture method in conjunction with matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) identification for detection of 5 different bacterial pathogens associated with porcine respiratory disea
Chan Hee Lee, Yong Ho Park, Youn Soo Chung, Yun Sung Hu, Chung Wung Kim, Youn Kyung Park, Suk Shin, Kun Taek Park 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 83 229-229 (1 pages)
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Identification of a Marker Protein for Cardiac Ischemia and Reperfusion Injury by Two-Dimensional Gel Electrophoresis and Matrix-Assisted Laser Desorption Ionization Mass Spectrometry
YoungsukLee, NariKim, HyunjuKim, HyunJoo, YoungnamKim, DaehoonJeong, DangVanCuong, EuiyongKim, DaeYoungHur, YoungShikPark, YongGeunHong, Sa 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.4 6 207-212 (6 pages)
to evaluate the expression of cardiac marker protein in rabbit cardiac tissue that was exposed to ischemic preconditioning (IPC), or ischemiareperfusion injury (IR) using two-dimensional gel electrophoresis (2DE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). We compared 2DE gels of control (uninjured) cardiac tissue with those of IPC and IR cardiac tissue. Expression of one protein was detected in IR heart tissue, however the protein was not detected in the sample... -
Current Classification of the Bacillus pumilus Group Species, the Rubber- Pathogenic Bacteria Causing Trunk Bulges Disease in Malaysia as Assessed by MLSA and Multi rep-PCR Approaches
Ainur Ainiah Azman Husni, Siti Izera Ismail, Noraini Md. Jaafar, Dzarifah Zulperi 한국식물병리학회 The Plant Pathology Journal 15 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 3호 4 243-257 (15 pages)
Bacillus pumilus is the causal agent of trunk bulges dis- ease affecting rubber and rubberwood quality and yield production. In this study, B. pumilus and other closely related species were included in B. pumilus group, as they shared over 99.5% similarity from 16S rRNA analysis. Multilocus sequence analysis (MLSA) of five housekeeping genes and repetitive elements-based poly- merase chain reaction (rep-PCR) using REP, ERIC, and BOX primers conducted to analyze the diversity and systematic... -
Natural Transmission of Canine Influenza Virus H3N2 From Cats to Dogs
Kyu Wook Kim, Son Il Pak, Dong Jun An 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 82 228-229 (2 pages)
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Isolation and immunomodulatory activity of bursal peptide, a novel bursal peptide from the chicken bursa of Fabricius
Xiao-Dong Liu, Yingjuan Qian, Yong-Sam Jung, Pu-Yan Chen 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 14 501-507 (7 pages)
The bursa of Fabricius (BF), which is unique to birds, serves as the central humoral immune organ and plays a significant role in B lymphocyte differentiation. In this study, a new bursal peptide (BP-IV) was isolated from BF, which promoted colony-forming unit pre-B formation and regulated B cell differentiation. BP-IV also exerted immunomodulatory effects on antigen-specific immune responses via both humoral and cellular immunity in chicken and mice that had been immunized with inactivated... -
Comparative proteomic analysis of proteins expression changes in the mammary tissue of cows infected with Escherichia coli mastitis
Xiao-wei Zhao, Yong-xin Yang, Dong-wei Huang, Guang-long Cheng, Hui-ling Zhao 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 2 253-263 (11 pages)
Cows infected with Escherichia (E.) coli usually experience severe clinical symptoms, including damage to mammary tissues, reduced milk yield, and altered milk composition. In order to investigate the host response to E. coli infection and discover novel markers for mastitis treatment, mammary tissue samples were collected from healthy cows and bovines with naturally occurring severe E. coli mastitis. Changes of mammary tissue proteins were examined using two-dimensional gel... -
Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus
Xiaoyu Deng, Yanlong Cong, Renfu Yin, Guilian Yang, Chan Ding, Shengqing Yu, Xiufan Liu, Chunfeng Wang, †, Zhuang Ding, 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 7 511-517 (7 pages)
Quantitative intensity analysis showed that the expression of 78 proteins increased more than two-fold. Thirty-five proteins exhibited consistent changes in expression and 13 were identified as unique proteins by matrix assisted laser desorption ionization-time of flight mass spectrometer/mass spectrometer including three that were down-regulated and 10 that were up-regulated. These proteins were sorted into five groups based on function: macromolecular biosynthesis, cytoskeleton organization,... -
Quantitation of meloxicam in the plasma of koalas (Phascolarctos cinereus) by improved high performance liquid chromatography
Benjamin Kimble, Kong Ming Li, Merran Govendir* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 2 7-14 (8 pages)
An improved method to determine meloxicam (MEL) concentrations in koala plasma using reversed phase high performance liquid chromatography equipped with a photo diode array detector was developed and validated. A plasma sample clean-up step was carried out with hydrophilic- lipophilic copolymer solid phase extraction cartridges. MEL was separated from an endogenous interference using an isocratic mobile phase [acetonitrile and 50 mM potassium phosphate buffer (pH 2.15), 45：55 (v：v)] on a...
