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01.

Development of a multiplex loop-mediated isothermal amplification assay to detect shiga toxin-producing Escherichia coli in cattle

Hee-Jin Dong, Ae-Ri Cho, Tae-Wook Hahn, Seongbeom Cho* 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 19 317-325 (9 pages)

Hee-Jin Dong, Ae-Ri Cho, Tae-Wook Hahn, Seongbeom Cho* 저
...
- 3,000원
A multiplex loop-mediated isothermal amplification (mLAMP) assay was developed for simultaneous detection of the stx1 and stx2 genes and applied for detection of shiga toxin-producing Escherichia coli (STEC) in cattle farm samples. Two target genes were distinguished based on Tm values of 85.03 ± 0.54C for stx1 and 87.47 ± 0.35C for stx2. The mLAMP assay was specific (100% inclusivity and exclusivity), sensitive (with a detection limit as low as 10 fg/μL), and quantifiable (R = 0.9313). The...
02.

Development of Molecular Marker to Detect Citrus Melanose Caused by Diaporthe citri from Citrus Melanose-like Symptoms

Cheol-Woo Choi, Kyung-Eun Jung, Min-Ju Kim, Su-Hyeon Yoon, Suk-Man Park, Seong-Beom Jin, Jae-Wook Hyun 한국식물병리학회 The Plant Pathology Journal 6 Pages

한국식물병리학회 The Plant Pathology Journal 2021, 37권 6호 17 681-686 (6 pages)

Cheol-Woo Choi, Kyung-Eun Jung, Min-Ju Kim, Su-Hyeon Yoon, Suk-Man Park, Seong-Beom Jin, Jae-Wook Hyun 저
...
- 3,000원
It is difficult to distinguish melanose and melanoses-like symptoms with the naked eye because they appear simi- lar. To accurately detect melanose symptoms caused by Diaporthe citri from melanose-like symptoms, we devel- oped PCR-based specific primers Dcitri by aligning the internal transcribed spacer (ITS) region of D. citri with the ITS of Diaporthe cytosporella, Diaporthe foeniculina, Colletotrichum gloeosporioides, Botrytis cinerea, Alter- naria citri, and Fusarium oxysporum found on...
03.

Development of PCR-Based Molecular Marker for Detection of Xanthomonas campestris pv. campestris Race 6, the Causative Agent of Black Rot of Brassicas

Khandker Shazia Afrin, Md Abdur Rahim, Mehede Hassan Rubel, Jong-In Park, Hee-Jeong Jung, Hoy-Taek Kim, Ill-Sup Nou 한국식물병리학회 The Plant Pathology Journal 10 Pages

한국식물병리학회 The Plant Pathology Journal 2020, 36권 5호 4 418-427 (10 pages)

Khandker Shazia Afrin, Md Abdur Rahim, Mehede Hassan Rubel, Jong-In Park, Hee-Jeong Jung, Hoy-Taek Kim, Ill-Sup Nou 저
...
- 3,000원
Xanthomonas campestris pv. campestris (Xcc), the pathogen of black rot which is the most destructive disease of Brassica vegetables throughout the world. Here, we reported two novel sequence-characterized amplified region (SCAR) markers (i.e., XccR6-60 and XccR6-67) for the detection of Xcc race 6 via re-alignment of the complete genome sequences of Xcc races/ strains/pathovars. The specificity of SCAR primer sets was verified by mean of PCR amplification using the genomic DNA template of Xcc...
04.

A Small GTPase RHO2 Plays an Important Role in Pre-infection Development in the Rice Blast Pathogen Magnaporthe oryzae

Teng Fu, Joon-Oh Kim, Joon-Hee Han, Adiyantara Gumilang, Yong-Hwan Lee, Kyoung Su Kim 한국식물병리학회 The Plant Pathology Journal 10 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 2 470-479 (10 pages)

Teng Fu, Joon-Oh Kim, Joon-Hee Han, Adiyantara Gumilang, Yong-Hwan Lee, Kyoung Su Kim 저
...
- 3,000원
outcomes suggested the involvement of MoRHO2 in cAMP-mediated appressorium development. ΔMorho2 mutation also delayed the development of appressorium-like structures (ALS) at hyphal tips on hydrophobic surface, which were also abnormally shaped. These results suggested that MoRHO2 is involved in morphological development of appressoria and ALS from conidia and hyphae, respectively. As expected, ΔMorho2 mutant was defective in plant penetration, but was still able to cause lesions, albeit at a...
05.

Development of a Rapid Detection Method for Potato virus X by Reverse Transcription Loop-Mediated Isothermal Amplification

한국식물병리학회 The Plant Pathology Journal 7 Pages

한국식물병리학회 The Plant Pathology Journal 2015, 31권 3호 3 219-225 (7 pages)

저
...
- 3,000원
The primary step for efficient control of viral diseases is the development of simple, rapid, and sensitive virus detection. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been used to detect viral RNA molecules because of its simplicity and high sensitivity for a number of viruses. RT-LAMP for the detection of Potato virus X (PVX) was developed and compared with conventional reverse transcription polymerase chain reaction (RT-PCR) to demonstrate its advantages over...
06.

The Current Incidence of Viral Disease in Korean Sweet Potatoes and Development of Multiplex RT-PCR Assays for Simultaneous Detection of Eight Sweet Potato Viruses

한국식물병리학회 The Plant Pathology Journal 9 Pages

한국식물병리학회 The Plant Pathology Journal 2014, 30권 4호 9 416-424 (9 pages)

저
...
- 3,000원
Sweet potato is grown extensively from tropical to temperate regions and is an important food crop worldwide. In this study, we established detection methods for 17 major sweet potato viruses using single and multiplex RT-PCR assays. To investigate the current incidence of viral diseases, we collected 154 samples of various sweet potato cultivars showing virus-like symptoms from 40 fields in 10 Korean regions, and analyzed them by RT-PCR using specific primers for each of the 17 viruses. Of the...
07.

A Short-chain Dehydrogenase/reductase Gene is Required for Infection-related Development and Pathogenicity in Magnaporthe oryzae

한국식물병리학회 The Plant Pathology Journal 9 Pages

한국식물병리학회 The Plant Pathology Journal 2010, 26권 1호 2 8-16 (9 pages)

저
...
- 3,000원
of MoSDR1 suggests that the gene is associated with pathogenicity and plays an important role in M. oryzae development. To understand the roles of MoSDR1, the deletion mutant ΔMosdr1 for the gene was obtained via homology-dependent gene replacement. As expected, ΔMosdr1 was nonpathogenic; moreover, the mutant displayed pleiotropic defects in conidiation, conidial germination, appressorium formation, penetration, and growth inside host tissues. These results suggest that MoSDR1 functions as a...
08.

Development of Diagnostic Technology of Xylella fastidiosa Using Loop-Mediated Isothermal Amplification and PCR Methods

Suyoung Kim, Yujin Park, Gidon Kim 한국식물병리학회 식물병연구 7 Pages

한국식물병리학회 식물병연구 2021, 27권 1호 6 38-44 (7 pages)

Suyoung Kim, Yujin Park, Gidon Kim 저
...
- 3,000원
Xylella fastidiosa is the most damaging pathogen in many parts of the world. To increase diagnostic capability of X. fastidiosa in the field, the loop-mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) assay were developed to mqsA gene of citrate-synthase (XF 1535) X. fastidiosa and evaluated for speci- ficity and sensitivity. Both assays were more robust than current published tests for detection of X. fastidiosa when screened against 16 isolates representing the four...
09.

Development of RT-PCR for the detection of Getah virus

Sung Suk Choi, Dong Kun Yang, Hyun Ye Jo, Ha Hyun Kim, Seung Heon Lee, In Soo Cho 대한수의학회 대한수의학회 학술대회발표집 2 Pages

대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 119 394-395 (2 pages)

Sung Suk Choi, Dong Kun Yang, Hyun Ye Jo, Ha Hyun Kim, Seung Heon Lee, In Soo Cho 저
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- 무료
10.

Toward the development of a one-dose classical swine fever subunit vaccine

Rachel F. Madera, Lihua Wang, Wenjie Gong, Yulia Burakova, Sterling Buist, Jerome Nietfeld, Jamie Henningson, Ada G. Cino-Ozuna, Changch 대한수의학회 Journal of Veterinary Science 13 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 9 393-405 (13 pages)

Rachel F. Madera, Lihua Wang, Wenjie Gong, Yulia Burakova, Sterling Buist, Jerome Nietfeld, Jamie Henningson, Ada G. Cino-Ozuna, Changch 저
...
- 4,200원
Highly contagious classical swine fever (CSF) remains a major trade and health problem in the pig industry, resulting in large economic losses worldwide. In CSF-endemic countries, attenuated CSF virus (CSFV) vaccines have been routinely used to control the disease. However, eradication of CSFV in a geographical area would require permanent reduction to zero presence of the virus. It is therefore of paramount importance to develop a safe, potent, and non-infectious CSF vaccine. We have previously...

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