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A simplified one-step nuclear transfer procedure alters the gene expression patterns and developmental potential of cloned porcine embryos
Sang Kyu Park, Sangho Roh*, Jong-Im Park* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 8 73-80 (8 pages)
(simplified one-step SCNT; SONT) or separately with different pipettes (conventional two-step SCNT; CTNT) as the control procedure. After blastocysts from both groups were cultured in vitro, the expression levels of developmentally important genes (OCT4, NANOG, EOMES, CDX2, GLUT-1, PolyA, and HSP70) were analyzed by real-time quantitative polymerase chain reaction. Both the developmental rate according to blastocyst stage as well as the expression levels CDX2, EOMES, and HSP70 were elevated with... -
Exploring Technological and Engineering Literacy Standards
Lee Choon-Sig 경인교육대학교 교육연구원 The Journal of Education 17 Pages
경인교육대학교 교육연구원 The Journal of Education 2020, Vol.3 No.2 1 1-17 (17 pages)
This study aims to explore the recently revised trend of technology-engineering literacy for students and children in the United States and the composition of benchmark contents by school level. The study can be used as basic data for revision of the technology education curriculum in Korea. In order to achieve the purpose of this study, literature research was mainly conducted. STL (Standards for Technological Literacy) and STEL (Standards for Technological and Engineering Literacy) published... -
“In the Shoes of an Algorithm”: A Media Education Game to Address Issues Related to Recommendation Algorithms
Jerry Jacques, J?r?my Grosman, Anne-Sophie Collard, Yeonju Oh, Amie Kim, Hyeon-Seon Jeong 경인교육대학교 교육연구원 The Journal of Education 26 Pages
경인교육대학교 교육연구원 The Journal of Education 2020, Vol.3 No.1 4 37-62 (26 pages)
Recommendation algorithms play an important role in the current media landscape by fostering interactions between users of digital media platforms and media content. The growing concern with recommendation algorithms raises important questions for media educators who emphasize critical analysis of media content and creative production and are required to develop media education initiatives focused on a critical understanding of digital platforms and their underlying technical infrastructures.... -
Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples
Hye Kwon Kim, Kwang Soo Lyoo, Thi My Le Huynh, Hyoung Joon Moon, Van Giap Nguyen, Bong Kyun Park 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 16 253-256 (4 pages)
There are high levels of co-incidence of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) in porcine tissue. This study established a duplex nested reverse transcriptase polymerase chain reaction (RT-PCR) method that targets the genomic RNA of type 2 PRRSV and the mRNA of PCV2 in infected tissues. The method amplified discriminative bands of 347 bp and 265 bp specific for type 2 PRRSV and PCV2, respectively. The limits of detection of the duplex... -
A serological study of severe fever with thrombocytopenia syndrome using a virus neutralization test and competitive enzyme-linked immunosorbent assay
Hyojin Lee, Eun-Ju Kim, In-Soo Cho, Jae-Young Song, Jeong Soo Choi, Ji Youn Lee, Yeun-Kyung Shin 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 1호 5 33-38 (6 pages)
Severe fever with thrombocytopenia syndrome (SFTS) is caused by the SFTS virus (SFTSV). The SFTSV appears to have a wide host range, as SFTSV-positive ticks have been isolated from both farm animals and wild rodents. Therefore, it is important to monitor SFTSV-positive animals to prevent the transmission of SFTSV from animals to humans. Previously, we developed a competitive enzyme-linked immunosorbent assay (cELISA) to detect SFTSV-specific antibodies from field animals and compared the cELISA... -
Application of vincristine-loaded platelet therapy in three dogs with refractory immune-mediated thrombocytopenia
Hyung-Jin Park, Ja-Won Kim, Kun-Ho Song, Kyoung-Won Seo 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 1호 17 127-130 (4 pages)
Three dogs presented with refractory immune-mediated thrombocytopenia (IMT). All patients failed to respond to prednisone, which is considered a mainstay of immunosuppressive therapy. Vincristine-loaded platelets (VLPs), which act selectively on mononuclear phagocytes,were introduced. After the VLPs were transfused, two dogs responded quickly withimproved clinical signs while the third dogwith recurrent IMT was euthanized due to its deteriorating condition. This case report... -
Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells
Min-Soo Seo, Yun-Hyeok Jeong, Jeung-Ran Park, Sang-Bum Park, Kyoung-Hwan Rho, Hyung-Sik Kim, Kyung-Rok Yu, Seung-Hee Lee, Ji-Won 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 2 181-187 (7 pages)
Human umbilical cord blood-derived mesenchymal stem cells (MSCs) are known to possess the potential for multiple differentiations abilities in vitro and in vivo. In canine system, studying stem cell therapy is important, but so far, stem cells from canine were not identified and characterized. In this study, we successfully isolated and characterized MSCs from the canine umbilical cord and its fetal blood. Canine MSCs (cMSCs) were grown in medium containing low glucose DMEM with 20% FBS. The... -
Disposition kinetics and urinary excretion of ciprofloxacin in goats following single intravenous administration
R. Raina, S. Prawez*, D.J. Dimitrova, N.K. Pankaj, P.K. Verma 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 3호 3 241-246 (6 pages)
We evaluated the pharmacokinetics of ciprofloxacin in serum (n = 6) and urine (n = 4) in goats following a single intravenous administration of 4 mg/kg body weight. The serum concentration-time curves of ciprofloxacin were best fitted by a two-compartment open model. The drug was detected in goat serum up to 12 h. The elimination rate constant (b) and elimination half-life (t1/2b) were 0.446 ± 0.04 h and 1.630 ± 0.17 h, respectively. The apparent volume of distribution at steady state...
