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A simplified one-step nuclear transfer procedure alters the gene expression patterns and developmental potential of cloned porcine embryos
Sang Kyu Park, Sangho Roh*, Jong-Im Park* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 8 73-80 (8 pages)
Various somatic cell nuclear transfer (SCNT) techniques for mammalian species have been developed to adjust species-specific procedures to oocyte-associated differences among species. Species-specific SCNT protocols may result in different expression levels of developmentally important genes that may affect embryonic development and pregnancy. In the present study, porcine oocytes were treated with demecolcine that facilitated enucleation with protruding genetic material. Enucleation and donor... -
Effects of Serotonin on the Induction of Long-term Depression in the Rat Visual Cortex
Hyun-JongJang, Kwang-HyunCho, , Sung-WonPark, Myung-JunKim, ShinHeeYoon, Duck-JooRhie 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2010, Vol.14 No.5 14 337-343 (7 pages)
Long-term potentiation (LTP) and long-term depression (LTD) have both been studied as mechanisms of ocular dominance plasticity in the rat visual cortex. In a previous study, we suggested that a developmental increase in serotonin [5-hydroxytryptamine (5-HT)] might be involved in the decline of LTP, since 5-HT inhibited its induction. In the present study, to further understand the role of 5-HT in a developmental decrease in plasticity, we investigated the effect of 5-HT on the induction of LTD... -
Heterogeneity of the SR-dependent Inward Na+-Ca2+ Exchange Current in the Heavily Ca2+-buffered Rat Ventricular Myocytes
Kyung-BongYoon, Sung-WanAhn, ChangMannKo 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.2 6 101-110 (10 pages)
Voltage-sensitive release mechanism was pharmacologically dissected from the Ca2-induced Ca2 release in the SR Ca2 release in the rat ventricular myocytes patch-clamped in a whole-cell mode. SR Ca2 release process was monitored by using forward-mode Na-Ca2 exchange after restriction of the interactions between Ca2 from SR and Na-Ca2 exchange within micro-domains with heavy cytosolic Ca2 buffering with 10 mM BAPTA. During... -
Validation and Application of a Real-time PCR Protocol for the Specific Detection and Quantification of Clavibacter michiganensis subsp. sepedonicus in Potato
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 2호 4 123-131 (9 pages)
Clavibacter michiganensis subsp. sepedonicus (Cms) multiplies very rapidly, passing through the vascular strands and into the stems and petioles of a diseased potato. Therefore, the rapid and specific detection of this pathogen is highly important for the effective control of the pathogen. Although several PCR assays have been developed for detection, they cannot afford specific detection of Cms. Therefore, in this study, a computational genome analysis was performed to compare the sequenced... -
Induction of Drought Stress Resistance by Multi-Functional PGPR Bacillus Licheniformis K11 in Pepper
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2013, 29권 2호 10 201-208 (8 pages)
Drought stress is one of the major yield affecting factor for pepper plant. The effects of PGPRs were analyzed in relation with drought resistance. The PGPRs inoculated pepper plants tolerate the drought stress and survived as compared to non-inoculated pepper plants that died after 15 days of drought stress. Variations in protein and RNA accumulation patterns of inoculated and noninoculated pepper plants subjected to drought conditions for 10 days were confirmed by two dimensional... -
Synthesize multi-walled carbon nanotubes via catalytic chemical vapour deposition method on Fe-Ni bimetallic catalyst supported on kaolin
Aliyu A, Abdulkareem AS, Kovo AS, Abubakre OK, Tijani JO, Kariim I 한국탄소학회 Carbon Letters 18 Pages
한국탄소학회 Carbon Letters 2017, Vol.21 5 33-50 (18 pages)
In this study, Fe-Ni bimetallic catalyst supported on kaolin is prepared by a wet impregnation method. The effects of mass of kaolin support, pre-calcination time, pre-calcination temperature and stirring speed on catalyst yields are examined. Then, the optimal supported Fe-Ni catalyst is utilised to produce multi-walled carbon nanotubes (MWCNTs) using catalytic chemical vapour deposition (CCVD) method. The catalysts and MWCNTs prepared using the optimal conditions are characterized using high... -
Calcium permeability of transient receptor potential canonical (TRPC) 4 channels measured by TRPC4-GCaMP6s
JuyeonKo, JongyunMyeong, DongkiYang, InsukSo 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.1 16 133-140 (8 pages)
whether the current-voltage (I-V) curve has a doubly rectifying shape or inward rectifying shape. To investigate the calcium permeability of TRPC4 channels, we attached GCaMP6s to TRPC4 and simultaneously measured the current and calcium signals. A TRPC4 specific activator, (–)-englerin A, induced both current and calcium fluorescence with the similar time course. Muscarinic receptor stimulator, carbachol, also induced both current and calcium fluorescence with the similar time course. By... -
Functional Expression of P2Y Receptors in WERI-Rb1 Retinoblastoma Cells
Na-HyunKim, Kyu-SangPark, JoonHyungSohn, Byung-IlYeh, ChangMannKo, InDeokKong 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 5 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2011, Vol.15 No.1 9 61-65 (5 pages)
P2Y receptors are metabotropic G-protein-coupled receptors, which are involved in many important biologic functions in the central nervous system including retina. Subtypes of P2Y receptors in retinal tissue vary according to the species and the cell types. We examined the molecular and pharmacologic profiles of P2Y purinoceptors in retinoblastoma cell, which has not been identified yet. To achieve this goal, we used Ca2＋ imaging technique and western blot analysis in WERI-Rb-1 cell, a human... -
Inhibitory Effect of Chitosan and Phosphate Cross-linked Chitosan against Cucumber Mosaic Virus and Pepper Mild Mottle Virus
Venkata Subba Reddy Gangireddygari, Bong Nam Chung, In-Sook Cho, Ju-Yeon Yoon 한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 6호 12 632-640 (9 pages)
Cucumber mosaic virus (CMV) and Pepper mild mot- tle virus (PMMoV) causes severe economic loss in crop productivity of both agriculture and horticulture crops in Korea. The previous surveys showed that naturally available biopolymer material – chitosan (CS), which is from shrimp cells, reduced CMV accumulation on pepper. To improve the antiviral activity of CS, it was synthesized to form phosphate cross-linked chitosan (PCS) and compared with the original CS. Initially, the activity of CS...
