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Detection of canine distemper virus (CDV) through one step RT-PCR combined with nested PCR
Yong-Hwan Kim, Kyu-Woan Cho, Hwa-Young Youn, Han Sang Yoo, Hong-Ryul Han 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 1호 10 59-63 (5 pages)
dogs. Of the 51 blood samples from dogs clinically suspected of CD, 45 samples were revealed as positive by one-step RT-PCR combined with nested PCR. However, only 15 samples were identified as positive with a single one step RT-PCR. Therefore approximately 60% increase in the efficiency of the diagnosis was observed by the combined method. These results suggested that one step RT-PCR combined with nested PCR could be a sensitive, specific, and practical method for diagnosis of CDV infection. -
Effects of forward & backward walking training with progressive body weight supported on stroke patients’ ambulatory ability
Kyung-Hoon Kim, Suk-Min Lee 물리치료재활과학회 Physical Therapy Rehabilitation Science 9 Pages
물리치료재활과학회 Physical Therapy Rehabilitation Science 2014, 제 3권 제 2호 1 77-85 (9 pages)
of the step length, total double support, cadence, gait were measured using optogait and the 10-m walk test (10MWT), 6 minutes walk test (6MWT). Results: In the within group comparisons, all the three groups showed significant differences between before and after the intervention (p<0.05). In the comparison of the three groups, there were significant differences among the three groups in stride length, double limb support stance, cadence, 10MWT, and 6MWT in the third week, and only in stride... -
Embodied Culture in Pottery: Developing Teacher’s Package for Understanding Other’s Culture and Own Culture
Kim Haikyung 경인교육대학교 교육연구원 The Journal of Education 23 Pages
경인교육대학교 교육연구원 The Journal of Education 2018, Vol.1 No.2 3 29-51 (23 pages)
There are various perspectives on the roles and functions of museums. In modern times, socio-educational functions of museums are the most emphasized. Most museums offer programs for schools, but teachers still prefer not to visit them. The reason why teachers feel pressured to visit a museum is that they lack knowledge about museum exhibits. Also, it takes too much time and energy for individual teachers to reconstruct the curriculum in conjunction with museum exhibits. The link with the school... -
Dembo polymerase chain reaction technique for detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs
Sayed Samim Rahpaya, Shinobu Tsuchiaka, Mai Kishimoto, Mami Oba, Yukie Katayama, Yuka Nunomura, Saki Kokawa, Takashi Kimura, Atsushi Kob 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 4 350-357 (8 pages)
Bovine abortion, diarrhea, and respiratory disease complexes, caused by infectious agents, result in high and significant economic losses for the cattle industry. These pathogens are likely transmitted by various vectors and reservoirs including insects, birds, and rodents. However, experimental data supporting this possibility are scarce. We collected 117 samples and screened them for 44 bovine abortive, diarrheal, and respiratory disease complex pathogens by using Dembo polymerase chain... -
Effects of induced endometritis on uterine blood flow in cows as evaluated by transrectal Doppler sonography
Letizia Debertolis, Gaetano Mari, Barbara Merlo, Sabine Merbach, Heinz-Adolf Schoon, Eleonora Iacono, Heinrich Bollwein 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 8 189-197 (9 pages)
This study was conducted to evaluate the effects of induced endometritis on uterine blood flow in cows. Transrectal Doppler sonography was performed on uterine arteries of six cyclic cows before and for 4 days after inducing acute endometritis by intrauterine infusion of 720 mg of policresulen, and for 4 days of the following estrous cycle. Time-averaged maximum velocity (TAMV) increased (p < 0.001) and pulsatility index (PI) decreased (p < 0.0001) within 1 h of policresulen... -
Quantitative real-time polymerase chain reaction for detecting Mycoplasma hyosynoviae and Mycoplasma hyorhinis in pen-based oral, tonsillar, and nasal fluids
João Carlos Gomes Neto, Leslie Bower, Barbara Z. Erickson, Chong Wang, Matthew Raymond, Erin L. Strait 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 9 195-201 (7 pages)
Mycoplasma (M.) hyorhinis and M. hyosynoviae are pathogens known to cause disease in pigs post-weaning. Due to their fastidious nature, there is increased need for culture-independent diagnostic platforms to detect these microorganisms. Therefore, this study was performed to develop and optimize quantitative real-time PCR (qPCR) assays to rapidly detect M. hyorhinis and M. hyosynoviae in pen-based oral fluids as well as nasal and tonsillar fluids as proxies for samples used in swine herd... -
Prostaglandin F receptor expression in intrauterine tissues of pregnant rats
Elvan Anadol*, Halit Kanca, Atiye Seda Yar, Fatma Helvacioğlu, Sevda Menevşe, Engin Ҫalgüne 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 14 125-131 (7 pages)
In this investigation, we studied the expression and localization of rat prostaglandin F (FP) receptor in uterine tissues of rats on gestational Days 10, 15, 18, 20, 21, 21.5 and postpartal Days 1 and 3 using Western blotting analysis, real-time PCR, and immunohistochemistry. A high level of immunoreactivity was observed on gestational Days 20, 21, and 21.5 with the most significant signals found on Day 20. FP receptor protein was expressed starting on gestational Day 15, and a fluctuating... -
Expression of verocytotoxic Escherichia coli antigens in tobacco seeds and evaluation of gut immunity after oral administration in mouse model
Luciana Rossi, Alessia Di Giancamillo, Serena Reggi, Cinzia Domeneghini, Antonella Baldi, Vittorio Sala, Vittorio Dell'Orto, Ann 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 5 263-270 (8 pages)
Verocytotoxic Escherichia (E.) coli strains are responsible for swine oedema disease, which is an enterotoxaemia that causes economic losses in the pig industry. The production of a vaccine for oral administration in transgenic seeds could be an efficient system to stimulate local immunity. This study was conducted to transform tobacco plants for the seed-specific expression of antigenic proteins from a porcine verocytotoxic E. coli strain. Parameters related to an immunological response and... -
Expression of KA1 kainate receptor subunit in the substantia gelatinosa of the trigeminal subnucleus caudalis in mice
Seon Ah Park, Soo Joung Park, Seong Kyu Han* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 4 299-304 (6 pages)
The KA1 kainate receptor (KAR) subunit in the substantia gelatinosa (SG) of the trigeminal subnucleus caudalis (Vc) has been implicated in the processing of nociceptive information from the orofacial region. This study compared the expression of the KA1 KAR subunit in the SG of the Vc in juvenile, prepubescent and adult mice. RT-PCR, Western blot and immunohistochemistry analyses were used to examine the expression level in SG area. The expression levels of the KA1 KAR subunit mRNA and protein... -
Genetic characterization of Barbari goats using microsatellite markers
J. Ramamoorthi, K. Thilagam, S. N. Sivaselvam*, S. M. K. Karthickeyan 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 11 73-76 (4 pages)
Genetic variation in Barbari goats, a highly prolific breed distributed widely in the northern part of India, known for better milk and meat quality, was studied as a part of genetic characterization and conservation. The genomic DNA from 50 unrelated Barbari goats were amplified via PCR with a panel of 21 microsatellite markers, and resolved through 6 per cent denaturing polyacrylamide gel electrophoresis followed by silver staining. The number of alleles ranged from 4 to 11, with allele sizes...
