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전체 선택해제
총 387건 검색
본문검색 결과만 보기
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Intrinsic Gating in Inward Rectifier Potassium Channels (Kir2.1) with Low Polyamine Affinity Generated by Site Directed Mutagenesis
ISo, , IAshmole, HSoh, CSPark, PJSpencer, MLeyl, PRStanfield, 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 12 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.3 3 131-142 (12 pages)
We have studied mutant forms of Kir2.1 in which an aspartate residue (D172), important for gating by intracellular polyamines, is replaced by one of three basic residues (Arg, Lys or His). Such channels are highly selective for K+, but show inward rectification that is a shallow function of voltage compared with that found in wild type. This inward rectification occurs with a reduced affinity for spermine and persists in the absence of polyamines. Though the unitary current-voltage relation... -
Effects of Site-Mutagenesis of an Amino Acid Triplet Repeat atM1 and M2 Muscarinic Receptors on Receptor Function
이석용(Seok-Yong Lee), 이상복(Sang-Bok Lee) 대한약리학회 대한약리학잡지 12 Pages
대한약리학회 대한약리학잡지 1996, 제 32권 제 3호 3 311-322 (12 pages)
in a transposed fashion (LYT-TYL) in the sequence of M1 receptors. In this work, we employed site-directed mutagenesis to investigate the possible significance of this unique sequence diversity for determining the distinct differential cellular function at the two receptor subtypes. Mutation of the LYTTYL sequence of M1 receptors to the corresponding M2 receptor LYTLYT sequence did not result in a significant change in the binding affinity of the agonist carbachol. The reverse mutation at the M2... -
Generation of an Arginine Auxotrophic Mutant of Colletotrichum acutatum as a Recipient Host for Insertional Mutagenesis
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2009, 25권 3호 1 205-212 (8 pages)
arginine auxotrophic mutant with a plasmid DNA carrying an intact copy of CaARG2, which was smaller than the deleted region in the mutant, not only caused random vector insertions in the fungal genome, but also recovered both hyphal growth and pathogenicity of the mutant to the wild-type level. Using this new selection system, we have successfully developed a restriction enzyme-mediated integration procedure, which would provide an economically efficient random mutagenesis method in C. acutatum. -
Insertional Transposon Mutagenesis of Xanthomonas oryzae pv. oryzae KXO85 by Electroporation
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2004, 20권 3호 12 229-233 (5 pages)
these mutants. Furthermore, transposon insertion sites in the mutant bacterial chromosome were determined by direct genomic DNA sequencing using transposon-specific primers with ABI 3100 Genetic Analyzer. Efficiency of transposition was influenced mostly by the competence status of X. oryzae pv. oryzae cells and the conditions of electroporation. These results indicated that the insertion mutagenesis strategy could be applied to define function of uncharacterized genes in X. oryzae pv. oryzae. -
Site-directed mutagenesis를 이용한 BCTV ORE L4의 기능 분석
이석찬, 박을용 한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 1998, 14권 5호 25 513-518 (6 pages)
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Construction of Modified Bacillus thuringiensis cry1Ac Genes for Transgenic Crop Through Multi Site-directed Mutagenesis
Xu. Hong Guang, Roh. Jong-Yul, Wang. Yong, Choi. Jae-Young, Shim. Hee-Jin, Liu. Qin, Tao. Xueying, Woo. Soo-Dong, Jin. Byung-Rae, Je. Yeo 한국잠사학회 International journal of industrial entomology 6 Pages
한국잠사학회 International journal of industrial entomology 2009, Vol.19 No.1 199-204 (6 pages)
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Isolation of a Tryptophan-Overproducing Strain Generated by EMS Mutagenesis of Candida rugosa
Kim. Sun-Hye, Ha. Yu-Mi, Youm. Hyoung-Joon, An. Gil-Hwan, Lee. Bong-Duk, Won. Mi-Sun, Song. Kyung-Bin 한국식품영양과학회 Journal of food science and nutrition 3 Pages
한국식품영양과학회 Journal of food science and nutrition 2004, Vol.9 No.2 187-189 (3 pages)
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Protein Engineering of Deoxynucleoside Kinase from Lactobacillus acidophilus: Effect of Site-Directed Mutagenesis on Microbial Growth
Park. Inshik, Kim. Eun-Ae, Bang. Keuk-Seung, Kim. Seok-Hwan, Kim. Gi-Nahm, Lee. Min-Kyung, Kil. Ji-Oeun 한국식품영양과학회 Journal of food science and nutrition 3 Pages
한국식품영양과학회 Journal of food science and nutrition 2001, Vol.6 No.1 79-81 (3 pages)
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Reduction of Bacterial Mutagenesis of 2-Amino-3-Methylimidazo[4,5-f]quinoline by S-9 Fraction from Mice Treated with Conjugated Linoleic Acid (CLA)
Park. Kyung-Ah, Kim. Seck-Jong, Park. Soo-Jahr, Park. Gu-Boo, Lim. Dong-Kil, Bahn. Kyeong-Nyeo, Cho. Yong-Un, Park. Jung H.Y., Pariza. M 한국식품영양과학회 Journal of food science and nutrition 5 Pages
한국식품영양과학회 Journal of food science and nutrition 2001, Vol.6 No.1 57-61 (5 pages)
