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Preparation and evaluation of enrofloxacin microspheres and tissue distribution in rats
Fan Yang, Jijun Kang, Fang Yang, Zhensheng Zhao, Tao Kong, Zhenling Zeng 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 4 157-164 (8 pages)
a preparation. Much attention is being given to targeting microspheres [9,21-23,29], especially ones that are biodegradable [7,18,26]. PLGA has been widely used to load ..PAGE:6 162 Fan Yang et al. Journal of Veterinary Science Table 4. The pharmacokinetic parameters of enrofloxacin in plasma and tissues after one single intravenous injection of the conventional ENR-INJ (7.5 mg/kg bw) in rats... New enrofloxacin microspheres were formulated, and their physical properties, lung-targeting ability, and tissue distribution in rats were examined. The microspheres had a regular and round shape. The mean diameter was 10.06 μm, and the diameter of 89.93% of all microspheres ranged from 7.0 μm to 30.0 μm. Tissue distribution of the microspheres was evaluated along with a conventional enrofloxacin preparation after a single intravenous injection (7.5 mg of enrofloxacin/kg bw). The results... -
Hangover relieving effect of Sanghwang mushroom mycelium cultured in germinated buckwheat
Yoo-jin An , Sung-min Cho , Min-su Kim , Hae-hee Moon , Dong- Soo Park , Nam-gen Jeon , Youngjae Lee , Chang-hoon Han 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 8 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2017, 제 57권 제 3호 1 147-154 (8 pages)
in hepato- cytes occurs via three major pathways: alcohol dehydroge- nase (ADH) pathway, microsomal ethanol oxidizing system, and catalase [24]. Alcohol is metabolized into acetaldehyde and acetic acid by several liver enzymes including ADH and acetaldehyde dehydrogenase (ALDH), respectively [29]. ALDH oxidizes more than 90% of acetaldehyde produced from alco- hol, transforming acetaldehyde into... -
Effects of Isoflurane Anesthesia on Post-Anesthetic Sleep-Wake Architectures in Rats
Hwan-SooJang, Ji-YoungJung, Kwang-HoJang, Maan-GeeLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2010, Vol.14 No.5 7 291-297 (7 pages)
bioelectric amplifier (Model 3500, A-M System, Inc., Carlsborg, WA, USA). The EEG signals were amplified× 10,000 and filtered over a range of 1 to 100 Hz. A 60-Hz notch filter was included. EMG signal was filtered over a range of 30 to 300 Hz and amplified by 10,000. They were displayed and sampled by an AD converter (DAQ Pad 6015, National Instruments Inc., Unio n City, CA, USA) controlled... The sleep homeostatic response significantly affects the state of anesthesia. In addition, sleep recovery may occur during anesthesia, either via a natural sleep-like process to occur or via a direct restorative effect. Little is known about the effects of isoflurane anesthesia on sleep homeostasis. We investigated whether 1) isoflurane anesthesia could provide a sleep-like process, and 2) the depth of anesthesia could differently affect the post-anesthesia sleep response. Nine rats were treated... -
Intestinal Absorption of Fibrinolytic and Proteolytic Lumbrokinase Extracted from Earthworm, Eisenia andrei
XiangMeiYan, Chung-HyoKim, ChulKyuLee, JangSikShin, IlHwanCho, UyDongSohn 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 5 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2010, Vol.14 No.2 2 71-75 (5 pages)
a 0.45 μM membrane filter. Filtrates were concentrated using an ultrafiltration system, desalted, concentrated, and lyophilized to obtain lyophilized powder (referred to as lumbrokinase filtrate). Lumbrokinase filtrate was resolved in 500 ml of 20 mM phosphate buffer (pH 7.4), filtered through a 0.45μM mem- brane filter, and loaded on a DEAE-toyopearl 650 resin col- umn (4.5×35 cm). After... To investigate the intestinal absorption of a fibrinolytic and proteolytic lumbrokinase extracted from Eisenia andrei, we used rat everted gut sacs and an in situ closed-loop recirculation method. We extracted lumbrokinase from Eisenia andrei, and then raised polyclonal antibody against lumbrokinase. Fibrinolytic activity and proteolytic activity in the serosal side of rat everted gut sacs incubated with lumbrokinase showed dose- and time-dependent patterns. Immunological results obtained by... -
Effects of Dopamine on Intracellular pH in Opossum Kidney Cells
KyungWooKang, YungKyuKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 5 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.3 11 187-191 (5 pages)
a filter wheel (Lambda 10-2, Sutter Instru- ment Co., Novato, CA, U.S.A.). The resulting emitted fluorescence light was collected with 530 nm filter, using a ×40 oil immersion lens of an inverted microscope (IX70, Olympus, Japan). The overall image sampling rate by using a cooled CCD camera (SensiCam, PCO Computer Optics GmbH, Ger- many) was 0.2 Hz. The 490 nm/440 nm emission ratio from the... amplitude of intracellular acidification by isosmotical Na removal from the bath medium in chronically dopamine-treated cells were much smaller than those in control. Although it seemed to be attenuated in Na-dependent pH regulation system, Na-dependent pHi recovery by NHE after intracelluar acid loading in the dopamine-treated groups was not significantly different from the control. The result is interpreted to be due to the balance between the stimulation effects of lower... -
Identification, Characterization, and Efficacy Evaluation of Bacillus velezensis for Shot-Hole Disease Biocontrol in Flowering Cherry
Viet-Cuong Han, Nan Hee Yu, Hyeokjun Yoon, Neung-Ho Ahn, Youn Kyoung Son, Byoung-Hee Lee, Jin-Cheol Kim 한국식물병리학회 The Plant Pathology Journal 16 Pages
한국식물병리학회 The Plant Pathology Journal 2022, 38권 2호 5 115-130 (16 pages)
0.22-μm pore size filter unit (Minisart hydrophilic syringe filter; Goettingen, Germany) to obtain cell-free culture filtrate (CFCF), which was then used for antimicrobial activity testing and stored at 20°C until further use. In vitro antimicrobial activity and minimum inhibi- tory concentration. During the screening of 403 bacterial isolates (refer to the Supplementary Methods), we found... Though information exists regarding the pathogenesis of the shot-hole disease (SH) in flowering cherry (FC), there has been a lack of research focusing on SH management. Therefore, here, we investigated the inhibitory activities of antagonistic bacteria against SH pathogens both in vitro and in vivo as well as their biochemical characteristics and bioactive compounds. Two biosurfactant- producing bacterial antagonists, identified as Bacillus velezensis strains JCK-1618 and JCK-1696, exhibited... -
Role of Two Sets of RND-Type Multidrug Efflux Pump Transporter Genes, mexAB-oprM and mexEF-oprN, in Virulence of Pseudomonas syringae pv. tabaci 6605
Yuki Ichinose, Takafumi Nishimura, Minori Harada, Ryota Kashiwagi, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Fumiko Taguchi, Daigo Takemoto, Hidenori Matsui 한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 2호 4 148-156 (9 pages)
an RND transporter system in P. syringae pv. syringae B301D, the PseABC efflux system, revealed that PseABC has an important role in secretion of syringomycin and syringopeptin, phytotoxins of this pathogen (Kang and Gross, 2005). However, the role of RND efflux pumps in the secretion of the plant antimicrobial metabolites is not clear yet. In this study, we generated two single mutants for... Pseudomonas syringae pv. tabaci 6605 has two mul- tidrug resistance (MDR) efflux pump transporters, MexAB-OprM and MexEF-OprN. To understand the role of these MDR efflux pumps in virulence, we generated deletion mutants, ∆mexB, ∆mexF, and ∆mexB∆mexF, and investigated their sensitivity to plant-derived antimicrobial compounds, antibiotics, and virulence. Growth inhibition assays with KB soft agar plate showed that growth of the wild-type (WT) was inhibited by 5 μl of 1 M... -
Bacterial Traits Involved in Colonization of Arabidopsis thaliana Roots by Bacillus amyloliquefaciens FZB42
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2013, 29권 1호 7 59-66 (8 pages)
in a gnotobiotic system using Arabidopsis seedlings grown in agar solidi- fied Murashige-Skoog medium. It has been already shown that results obtained with FZB42 grown in gnotobiotic systems and soil systems are comparable (Fan et al., 2012). Material and Methods Bacterial strains and growth conditions. The bacterial strains, used in this study, are listed in Table 1. The bacteria were cultivated... (Fan et al., 2012). In order to extend these studies and to elucidate which genes are crucial for root colonization, we applied targeted mutant strains to Arabidopsis seedlings. The fates of root colonization in mutant strains impaired in synthesis of alternative sigma factors, non-ribosomal synthesis of lipopeptides and polyketides, biofilm formation, swarming motility, and plant growth promoting activity were analyzed by confocal laser scanning microscopy. Whilst the wildtype strain heavily... -
초청정 클린룸 공조방식에 따른 기류특성에 관한 수치해석
노광철, 이승철, 오명도 대한설비공학회 설비공학논문집 11 Pages
대한설비공학회 설비공학논문집 2003, Vol.15 No.9 751-761 (11 pages)
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A Computational Model of Cytosolic and Mitochondrial [Ca2＋] in Paced Rat Ventricular Myocytes
JaeBoumYoum, SeongWooChoi, ChangHanJang, HyoungKyuKim, ChaeHunLeem, NariKim, JinHan 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 23 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2011, Vol.15 No.4 5 217-239 (23 pages)
at 1.0 ∼5.0 kHz, 4-pole Bessel-type low-pass filter and were sampled at a rate of 1∼3 kHz. Electrode resistance before sealing was 3∼4 MΩ with K ＋ -rich recording solution, and it was around 5 G Ω after sealing. The liquid-junction potentials between normal Tyrode and pi pette solution, which were calculated based on ionic mobilities, were ＜5 mV. Temperatures were 22∼25 oC during... We carried out a series of experiment demonstrating the role of mitochondria in the cytosolic and mitochondrial Ca2＋ transients and compared the results with those from computer simulation. In rat ventricular myocytes, increasing the rate of stimulation (1∼3 Hz) made both the diastolic and systolic [Ca2＋] bigger in mitochondria as well as in cytosol. As L-type Ca2＋ channel has key influence on the amplitude of Ca2＋-induced Ca2＋ release, the relation between stimulus frequency and the...