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Effect of Water Temperature and Salinity on the Fertilized Egg Development and Larval Development of Sevenband Grouper, Epinephelus septemfasciatus
Song. Young-Bo, Lee. Chi-Hoon, Kang. Hyeong-Cheol, Kim. Hyung-Bae, Lee. Young-Don 한국발생생물학회 발생과 생식 9 Pages
한국발생생물학회 발생과 생식 2013, Vol.17 No.4 369-377 (9 pages)
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Simultaneous Determination of Veterinary Medicine in Pork, Egg, and Milk by Liquid Chromatography-Tandem Mass Spectrometry
Jin A Park, Seong Kwan Kim, Dan Zhang, Dana Jeong, Sang Hyun Cho, Ho Chul Shin 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 146 214-215 (2 pages)
vitro fertilized (IVF) embryo development. Materials and Methods: We investigated nuclear maturation, intracellular gluthatione (GSH), reactive oxygen species (ROS) levels, sperm penetration(SP) analysis and ensuing embryo development after PA and IVF. Each concentration (0, 1, 10, and 100 ng/ml)of GDF8 was supplemented in maturation medium during in vitro maturation. Data were analyzed by... -
Development of in vitro produced porcine embryos according to serum types as macromolecule
Jungmin Son, Don Buddika Oshadi Malaweera, Eunsong Lee, Sangtae Shin, Jongki Cho 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 11 315-321 (7 pages)
5 mL/L OEP (or vs. paste), 0.02 g/L gentamycin sulfate and 200 mL/L ..PAGE:3 Porcine embryonic development by serum types 317 egg yolk] and diluted to provide 1.0 × 10 9 sperm/mL. Next, semen was cooled to 5 o C over a 1h period and one volume of BF5 + 2% (v/v) glycerol (G-9012) (second diluted solution) was mixed with one volume of cooled semen. Straws (IMV Technologies, France) were... without gonadotrophin (GTH) and development rates of parthenogenetic activation (PA) and in vitro fertilization (IVF) embryos were then compared. The maturation rates of the PGS group was significantly higher when GTH was not added. Additionally, during development of PA embryos without GTH, the PGS group showed significantly higher cleavage and blastocyst formation rates. Moreover, the cleavage rates of IVF embryos were significantly higher in the PGS group, with no significant differences in... -
Dynamic analysis of Ca level during bovine oocytes maturation and early embryonic development
Su Li Liang, Qian Jun Zhao, Xiang Chen Li, Ya Ping Jin, Yi Peng Wang, Xiao Hua Su, Wei Jun Guan, Yue Hui Ma 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 5 133-142 (10 pages)
Kopf GS. 2 Molecular basis of mammalian egg activation. Curr Top Dev Boil 1995, 30, 21-62. 35. Steinhardt R, Zucker R, Schatten G. Intracellular calcium release at fertilization in the sea urchin egg. Dev Biol 1977, 58, 185-196. 36. Steinhardt RA, Epel D. Activation of sea-urchin eggs by a calcium ionophore. Proc Natl Acad Sci USA 1974, 71, 1915- 1919. 37. Stricker SA, Smythe TL. Endoplasmic... Mammalian oocyte maturation and early embryo development processes are Ca-dependent. In this study, we used confocal microscopy to investigate the distribution pattern of Ca and its dynamic changes in the processes of bovine oocytes maturation, in vitro fertilization (IVF), parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) embryo development. During the germinal vesicle (GV) and GV breakdown stage, Ca was distributed in the cortical ooplasm and throughout the oocytes from... -
Effect of IP3 and ryanodine treatments on the development of bovine parthenogenetic and reconstructed embryos
Gook-jun Ahn, Byeong-chun Lee, Woo-suk Hwang 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 11 131-137 (7 pages)
activation. Curr. Top. Dev. Biol. 1995, 30, 21-62. 25.Shinna, Y., Kaneda, M., Matsuyama, K., Tanaka, K., Hiroi, M. and Doi, K. Role of the extracellular Ca 2+ on the intracellular Ca 2+ changes in fertilized and activated mouse oocytes. J. Reprod. Fertil. 1993, 97, 143-150. 26.Sun, F. Z., Hoyland, J., Haung, X., Mason, W. and Moor, R. M. A comparison of intracellular changes in porcine eggs after... followed by 6-DMAP treatment. No significant differences were observed at any stage of embryonic development and none of the embryos activated by electroporation reached either the morula or blastocyst stage. However, 3.8% and 1.9% of embryos activated by microinjection sucessfully developed to the morula and blastocyst stages, respectively. In conclusion, activation treatments using IP3 and ryanodine are able to support the development of bovine parthenogenetic and reconstructed embryos. -
Original Article : Expression analysis of ciliary rootlet coiled coil protein mRNA during Xenopus development
Md. Mahfujur Rahman , In Shik Kim , Dong Choon Ahn , Ho Seong Cho , Won Il Kim , Bumseok Kim , Gee Wook Shin , Jungkee K 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 4 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2015, 제 55권 제 3호 5 181-184 (4 pages)
of the Development from the Fertilized Egg Till the End of Metamorphosis. North-Holland, Amsterdam, 1967. 7. McClintock TS, Glasser CE, Bose SC, Bergman DA. Tissue expression patterns identify mouse cilia genes. Physiol Genomics 2008, 32, 198-206. 8. Park TJ, Mitchell BJ, Abitua PB, Kintner C, Wallingford JB. Dishevelled controls apical docking and planar polarization of basal bodies in ciliated... -
Calcium Current in the Unfertilized Egg of the Hamster
Haan, Jae-Hee, Cho, Soo-Wan, Yang, Young-Sun, Park, Young-Geun, Park, Hong-Gi, Chang, Gyeong-Jae, Kim, Yang-Mi, Park, Choon-Ok, Hong, Se 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 2호 12 215-224 (10 pages)
it seems possible that this type of icaz+ might exist up to the I-cell stage after fertilization. In the mouse egg, ic 82+ with an 1-V relation similar to that of ic 82+ in the unfertilized egg was reported (Hong et al, 1992), suggesting that icaz+ in hamster eggs may exist at least till I-cell stage if they have a comparable progress in embryonic... specific channel was examined in the unfertilized hamster egg using the whole-cell voltage clamp technique. Pure inward current was isolated using a Ca2+-rich pipette solution containing 10 mM TEA. This current was independent of external Na+ and was highly sensitive to the Ca2+ concentration in the bathing solution, indicating that the inward current is carried by Ca2+. The maximal amplitude was -4.12±0.58nA (n=12) with 10mM Ca2+ at -3OmV from a holding potential of -8OmV. This current reached... -
Development of a 15-day Interval Spraying Program for Controlling Major Apple Diseases
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2008, 24권 4호 10 439-446 (8 pages)
of Plant Pathology Development of a 15-day Interval Spraying Program for Controlling Major Apple Diseases Dong Hyuck Lee 2, Dae Hee Kim 1, Ho Cheol Shin 3 and Jae Youl Uhm 1* 1School of Applied Biology and Chemistry, Kyungpook National University, Daegu 702-701, Korea 2Apple Experiment Station, National Horticultural Research Institute, Gunwi 716-812, Korea 3 Central Research Institute, Kyung... A fungicidal spray program for effective control of three major apple diseases in Korea (white rot, bitter rot, and Marssonina blotch) was developed. This was based on our previous studies showing that application of ergosterol biosynthesis inhibitors (EBIs) in early or mid-August can eradicate white rot infection in fruit and that some protective fungicides show after-infection activity against white rot. The basic spray program focused on control of white rot, the main target disease, and the... -
Analysis of swine leukocyte antigen class I gene profiles and porcine endogenous retrovirus viremia level in a transgenic porcine herd inbred for xenotransplantation research
Daria Matczyń, ska, Daniel Sypniewski, Sabina Gałka, Dagna Sołtysik, Tomasz Loch, Ewa Nowak, Zdzisław Smorą, g, Ilona Bednarek1 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 8 384-392 (9 pages)
fertilized egg cell [18]. The transgenic herd was obtained via Developmental Project N R12 0036 06/2009 (The National Centre for Research and Development, Poland) entitled: Obtaining of transgenic pigs as donors of tissue and organs for transplantation in humans and their biotechnological, physiological and medical characteristics (leader Prof. Ph.D. Z. Smorg). This study was approved by the... Molecular characterization of swine leukocyte antigen (SLA) genes is important for elucidating the immune responses between swine-donor and human-recipient in xenotransplantation. Examination of associations between alleles of SLA class I genes, type of pig genetic modification, porcine endogenous retrovirus (PERV) viral titer, and PERV subtypes may shed light on the nature of xenograft acceptance or rejection and the safety of xenotransplantation. No significant difference in PERV gag RNA level... -
A comparative study of Sephadex, glass wool and Percoll separation techniques on sperm quality and IVF results for cryopreserved bovine semen
Hae-Lee Lee, Sue-Hee Kim, Dong-Beom Ji, Yong-Jun Kim* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 11 249-255 (7 pages)
by inducing apoptosis [13]. Therefore, glass wool filtration might improve embryonic development by recovering spermatozoa with normal DNA in cryopreserved bovine semen compared with other treatment groups. However, further studies are required to determine whether glass wool filtration could remove more DNA-damaged spermatozoa than Percoll separation and have positive effects on developing... The aim of this study was to compare the effects of spermatozoa separation techniques on sperm quality and in-vitro fertilization (IVF) results for cryopreserved bovine semen. Sephadex, glass wool and Percoll gradient separation techniques were used for sperm separation and sperm motility, morphology and membrane integrity were evaluated before and after separation. Also, cleavage and blastocyst developmental rate were investigated after IVF with sperm recovered by each separation technique. The...