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Quantitation of meloxicam in the plasma of koalas (Phascolarctos cinereus) by improved high performance liquid chromatography
Benjamin Kimble, Kong Ming Li, Merran Govendir* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 2 7-14 (8 pages)
a Shimadzu class VP data system (ver. 7.4; Shimadzu, Japan) was used for chromatographic control, data collection, and data processing. The total run time for each sample was 15 min. Preparation of standards and quality control (QC) samples A primary stock standard solution of MEL (0.1 mg/mL) was prepared in 50% methanol and further diluted with 50% methanol to obtain a series of working... An improved method to determine meloxicam (MEL) concentrations in koala plasma using reversed phase high performance liquid chromatography equipped with a photo diode array detector was developed and validated. A plasma sample clean-up step was carried out with hydrophilic- lipophilic copolymer solid phase extraction cartridges. MEL was separated from an endogenous interference using an isocratic mobile phase [acetonitrile and 50 mM potassium phosphate buffer (pH 2.15), 45：55 (v：v)] on a... -
Control Efficacy of Bacillus velezensis AFB2-2 against Potato Late Blight Caused by Phytophthora infestans in Organic Potato Cultivation
Min Jeong Kim, Chang Ki Shim, Jong-Ho Park 한국식물병리학회 The Plant Pathology Journal 16 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 6호 8 580-595 (16 pages)
of biological control results are low. Biological control is an alternative technique to synthetic chemical fungicides in control of plant diseases, in order to create a more sustainable agricultural system Conflicts of Interest No potential conflict of interest relevant to this article was reported. Acknowledgments This study was carried out with the support of the Research Program for... an average inhibitory zone of 10.2 mm or more. Among the concentrations of AFB2-2, 107 cfu/ml showed the highest control value of 85.7% in the greenhouse. Among the three concentrations of AFB2-2, the disease incidence and severity of potato late blight at 107 cfu/ml was lowest at 0.07 and 6.7, respectively. The nucleotide sequences of AFB2-2 strain were searched in the NCBI GenBank; Bacillus siamensis strain KCTC 13613, Bacillus velezensis strain CR-502, and Bacillus amylo- liquefaciens strain... -
Preparation of Nanomaterial Wettable Powder Formulations of Antagonistic Bacteria from Phellodendron chinense and the Biological Control of Brown Leaf Spot Disease
Yanling Zeng, Han Liu, Tianhui Zhu, Shan Han, Shujiang Li 한국식물병리학회 The Plant Pathology Journal 17 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 3호 2 215-231 (17 pages)
(OD 600) was used to monitor the growth of bacteria. The fermentation broth of biocontrol was col- lected, and sterile liquid medium was used as the control. The absorbance at 600 nm was measured (Storek et al., 2019), and the growth of biocontrol bacteria was recorded. The Oxford cup method was used determine the bacterio- static effects (He et al., 2018). The Oxford cup was placed in a... quality test results for the wettable powder were obtained: wetting time 87.0 s, suspension rate 80.33%, frequency of microbial contamination 0.08%, pH 7.2, fineness 95.8%, drying loss 1.47%, and storage stability ≥83.5%. A pot experiment revealed that the ability of JKB05 to prevent fungal infections on P. chinense increased considerably and achieved levels of control as high as 94%. The use of nanomaterials significantly improved the ability of biocontrol bacteria to control this disease. -
Effect of Producing Different Phenazines on Bacterial Fitness and Biological Control in Pseudomonas chlororaphis 30-84
한국식물병리학회 The Plant Pathology Journal 15 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 1호 5 44-58 (15 pages)
P. chloro- raphis 30-84 phenazine promoter (PPsP) with 5-fold higher expression under the control of the quorum sensing system PhzR/PhzI (Yu, 2016). In order to create a plasmid contain- ing phzM and phzS together, primer pair phzM1-phzM3 was used to create a phzM amplicon with BamHI restric- tion enzyme sites at both ends of the PCR product. This amplicon was cloned into the BamHI site in... biological control agent selected for its ability to suppress diseases caused by fungal pathogens. P. chlororaphis 30-84 produces three phenazines: phenazine-1-carboxylic acid (PCA), 2-hydroxy-phenazine-1-carboxylic acid (2OHPCA) and a small amount of 2-hydroxy-phenazine (2OHPHZ), and these are required for fungal pathogen inhibition and wheat rhizosphere competence. The two, 2-hydroxy derivatives are produced from PCA via the activity of a phenazine-modifying enzyme encoded by phzO. In addition... -
Genetic Variation and Biological Control of Fusarium graminearum Isolated from Wheat in Assiut-Egypt
한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 2호 8 145-156 (12 pages)
new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor. Appl. Genet. 103:455-461. Mahmoud, A. F. and Abo-Elyousr, K. 2014. Genetic diversity and biological control of Rhizoctonia solani associated with root rot of soybean in Assiut governorate, Egypt. J. Plant Physiol. Pathol. 2:1-5. Manning, B., Southwell, R., Hayman, P. and Moore, K.... gain insight into biological control effect in situ, highly antagonistic isolates of T. harzianum and B. subtilis were selected, based on their in vitro effectiveness, for greenhouse test. It was revealed that T. harzianum and B. subtilis significantly reduced FHB severity. The obtained results indicated that T. harzianum and B. subtilis are very effective biocontrol agents that offer potential benefit in FHB and should be harnessed for further biocontrol applications. The accurate analysis of... -
A Synergistic Effect of Chitosan and Lactic Acid Bacteria on the Control of Cruciferous Vegetable Diseases
Yu-Chen Lin, Kuang-Ren Chung, Jenn-Wen Huang 한국식물병리학회 The Plant Pathology Journal 13 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 2호 5 157-169 (13 pages)
biological control measures using beneficial microorganisms. Beneficial mi- croorganisms are present ubiquitously in diverse environ- ments including phyllosphere, rhizosphere, food products, and agricultural wastes. Biological control using beneficial microorganisms has been reported with limited success on cruciferous vegetables (Hassan et al., 2017; Huang et al., 2018). In addition, there... Two lactic acid bacteria (LAB) designated J02 and J13 were recovered from fermented vegetables based on their ability to suppress soft rot disease caused by Pectobacterium carotovorum subsp. carotovorum (Pcc) on radish. J02 and J13 were identified as Lactobacillus pentosus and Leuconostoc fallax, respectively. The abil- ity of J02 and J13 to suppress plant diseases is highly dependent on chitosan. LAB alone has no effect and chitosan alone has only a moderate effect on disease reduction.... -
Open Access ： Enhanced Biological Control of Phytophthora Blight of Pepper by Biosurfactant-Producing Pseudomonas
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2013, 29권 4호 7 418-426 (9 pages)
the host plant root system. Appl. Environ. Microbiol. 68:3795 Qiu, S. X., He, H., Ruan, H. C., Guan, X. and Hu, F. P. 2004. Bio- logical control of pepper Phytophthora blight by endophytic TB2 (Bacillus sp.). Acta Phytopathol. Sin. 34:173179. Robles-Yerena, L., Rodriguez-Villarreal, R. A., Ortega-Amaro, M. A., Fraire-Velazquez, S., Simpson, J., Rodriguez-Guerra, R. and Jimenez-Bremont, J. F. 2010.... per plant) significantly enhanced biological control activity, resulting in a reduction of disease level ranging from 56.8 to 81.1%. ASM + mefenoxam was the most effective treatment while ASM alone was less effective in both bioassays. These results indicate that our Pseudomonas fluorescens strains (6L10, 6ba6 and 3ss9) that have biosurfactant-producing abilities are effective against P. capsici on pepper, and enhanced disease suppression could be achieved when they were used in combination with... -
Cell proliferation and neuroblast differentiation in the dentate gyrus of high-fat diet-fed mice are increased after rosiglitazone treatment
Dae Young Yoo, Woosuk Kim, Dae Won Kim, Sung Min Nam, Hyo Young Jung, Jong Whi Kim, Choong Hyun Lee, Jung Hoon Choi, Moo-Ho Won, 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 3 27-33 (7 pages)
HFD feeding and drug treatment Six-week-old mice were individually caged and allowed to adapt to a chow diet for 1 week. After this time, the mice were fed a commercial LFD (D12450Bi used as a control diet for D12492, n = 34; Research Diets, USA) or HFD (D12492i, n = 34; Research Diets) for 8 weeks. Four weeks after initiating the LFD and HFD feeding, vehicle (0.1% methyl cellulose) or 2... the subgranular zone of the dentate gyrus using Ki67 and doublecortin (DCX), respectively, as markers. In addition, we monitored the effects of RSG on the levels of DCX and brain-derived neurotrophic factor (BDNF) in hippocampal homogenates. At 8 weeks after the LFD feeding, the numbers of Ki67- and DCX-positive cells as well as hippocampal levels of DCX and BDNF were significantly decreased in the RSG-treated group compared to the vehicle-treated animals. In contrast, the numbers of Ki67- and... -
Development and standardization of a monoclonal antibody-based rapid flow-through immunoassay for the detection of Aphanomyces invadans in the field
B. Adil, K. M. Shankar, B. T. Naveen Kumar, Rajreddy Patil, Abhiman Ballyaya, K. S. Ramesh, Sathish Rama Poojary, Omkar V. Byadg 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 6 413-419 (7 pages)
on the upper side of the membrane above the control line. Homogenate from fish with EUS was dotted on the membrane below the control line. The samples on the membrane were air-dried for 5 min before the membrane was sequentially treated ..PAGE:3 Flow through immunoassay 415 with 200 μL each of blocking buffer (5% bovine serum albumin, BSA in 50 mM PBS, pH 7.2; Sigma, USA), anti-A. invadans... test zone at the center. The FTA could be completed within 10 min. Clear purple dots against a white background indicated the presence of Aphanomyces (A.) invadans. The FTA limit of detection was 7 μg/mL for A.invadans compared to 56 μg/mL for the immunodot. FTA and polymerase chain reaction (PCR) could detect A.invadans in fish tissue homogenates at a 10 dilution compared to a 10 dilution by immunodot. In fish suffering from natural cases of epizootic ulcerative syndrome (EUS) collected from... -
In vitro susceptibility of Staphylococcus aureus strains isolated from cows with subclinical mastitis to different antimicrobial agents
Ayman El Behiry*, Gerd Schlenker, Istvan Szabo, Uwe Roesler 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 6 153-161 (9 pages)
ID 32 API system, and genotypically using PCR and MALDI-TOF-MS (Table 1). Susceptibility of S. aureus to the nonoxinol-9 iodine complexes and chlorhexidine digluconate was measured using the broth macrodilution method. The MIC values of nonoxinol-9 iodine for the control group ranged from 41 to 46%. These values ranged from 41 to 49% for the dipped group. The mean MIC values of nonoxinol-9... Sensitivity to commercial teat dips (nonoxinol-9 iodine complex and chlorhexidine digluconate) of 56 Staphylococcus (S.) aureus strains isolated from quarter milk samples of various German dairy herds treated with different teat dipping schemes was investigated in this study. The minimum inhibitory concentration was determined using a broth macrodilution method according to the German Veterinary Association guidelines. The main objective of the current study was to induce in vitro resistance...