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Effects of Histamine on Cultured Interstitial Cells of Cajal in Murine Small Intestine
ByungJooKim, YoungKyuKwon, EuiyongKim, InsukSo 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2013, Vol.17 No.2 6 149-156 (8 pages)
from oth- er cell types in cultures, they were identified by phase con- trast microscopy after incubation with anti- c-kit antibody. Patch-clamp experiments The physiological salt solution used to bathe cells (Na + - Tyrode) contained; 5 mM KCl, 135 mM NaCl, 2 mM CaCl 2, 10 mM glucose, 1.2 mM MgCl 2, and 10 mM HEPES, ad- justed to pH 7.4 with NaOH. The pipette solution contained 140 mM KCl, 5... Interstitial cells of Cajal (ICCs) are the pacemaker cells in the gastrointestinal tract, and histamine is known to regulate neuronal activity, control vascular tone, alter endothelial permeability, and modulate gastric acid secretion. However, the action mechanisms of histamine in mouse small intestinal ICCs have not been previously investigated, and thus, in the present study, we investigated the effects of histamine on mouse small intestinal ICCs, and sought to identify the receptors... -
Activation of the cGMP/Protein Kinase G Pathway by Nitric Oxide Can Decrease TRPV1 Activity in Cultured Rat Dorsal Root Ganglion Neurons
YunjuJin, JunKim, JiyeonKwak 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2012, Vol.16 No.3 10 211-217 (7 pages)
- and Ca 2＋ -free Hank’s balanced salt solution (HBSS; Gibco, Invitrogen) and incubated in 2.5 mg/ml trypsin (Gibco, Invitrogen) at 37 o C for 30 min. Subsequently, the ganglia were centri- fuged (1,000 rpm, 10 min) and the pellet was washed two or three times with culture medium to inhibit the enzyme. The pellet was then resuspended in culture medium by gen- tle trituration with a Pasteur... Recent studies have demonstrated that nitric oxide (NO) activates transient receptor potential vanilloid subtype 1 (TRPV1) via S-nitrosylation of the channel protein. NO also modulates various cellular functions via activation of the soluble guanylyl cyclase (sGC)/protein kinase G (PKG) pathway and the direct modification of proteins. Thus, in the present study, we investigated whether NO could indirectly modulate the activity of TRPV1 via a cGMP/PKG-dependent pathway in cultured rat dorsal root... -
Bile Acid Inhibition of N-type Calcium Channel Currents from Sympathetic Ganglion Neurons
HyeKyungLee, KyoungHwaLee, Eui-SicCho 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2012, Vol.16 No.1 4 25-30 (6 pages)
on the nervous system. High conc entration of bile acids free in circulation may affect the function of peripheral and/or central neurons. In the present study, we explored this pos- sibility by studying the effects of bile acids on neuronal (N)-type Ca 2＋ channel that is known to be essential for neu- rotransmitter release at synapses of the peripheral and cen- tral nervous system [21].... Under some pathological conditions as bile flow obstruction or liver diseases with the enterohepatic circulation being disrupted, regurgitation of bile acids into the systemic circulation occurs and the plasma level of bile acids increases. Bile acids in circulation may affect the nervous system. We examined this possibility by studying the effects of bile acids on gating of neuronal (N)-type Ca2＋ channel that is essential for neurotransmitter release at synapses of the peripheral and central... -
Modern Methods for Analysis of Antiepileptic Drugs in the Biological Fluids for Pharmacokinetics, Bioequivalence and Therapeutic Drug Monitoring
JuseopKang, Yoo-SinPark, Shin-HeeKim, Sang-HyunKim, Min-YoungJun 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 16 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2011, Vol.15 No.2 1 67-82 (16 pages)
thalamic neurons (T-currents) [62]. Because side effects are often not corre- lated with high plasma conc entrations [63], TDM of ETX is not common due to its low protein binding and to the infrequent incidence of absenc e seizures [64]. But TDM can be useful for individualizing therapy with ETX, although in most cases therapy can be optimized simply on the basis of clinical response and EEG... Epilepsy is a chronic disease occurring in approximately 1.0% of the world's population. About 30% of the epileptic patients treated with availably antiepileptic drugs (AEDs) continue to have seizures and are considered therapy-resistant or refractory patients. The ultimate goal for the use of AEDs is complete cessation of seizures without side effects. Because of a narrow therapeutic index of AEDs, a complete understanding of its clinical pharmacokinetics is essential for understanding of the... -
P2X and P2Y Receptors Mediate Contraction Induced by Electrical Field Stimulation in Feline Esophageal Smooth Muscle
YoungRaeCho, HyeonSoonJang, WonKim, SunYoungPark, UyDongSohn 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2010, Vol.14 No.5 10 311-316 (6 pages)
ABBREVIATIONS: EFS, electrical field stimulation; CGRP, calcito- nin gene-related peptide; αβMeATP, αβ-methylene 5'-adenosine triphosphate; a ADPβS, denosine 5'-[β-thio]diphosphate trilithium salt; NANC, non-adrenergic, non-cholinergic; NO, nitric oxide. Received August 28, 2010, Revised October 7, 2010, Accepted October 20, 2010 Corresponding to: Uy Dong Sohn, Department of Pharmacology... a dose-dependent manner. P2Y agonist adenosine 5'-[Ղ-thio]diphosphate trilithium salt (ADPՂS, 10−7∼10−5 M) also potentiated EFS-induced contractions in a dose-dependent manner. Ecto-ATPase activator apyrase (5 and 10 U/ml) reduced EFS-induced contractions. Inversely, 6-N,N-diethyl-D-Ղ,Ճ- dibromomethylene 5'-triphosphate triammonium (ARL 67156, 10−4 M) increased EFS-induced contraction. These data suggest that endogenous ATP plays a role in EFS-induced... -
Dietary Ascorbate Supplementation Reduces Oxidative Tissue Damage and Expression of iNOS in the Kidney of Streptozotocin Induced Diabetic Rats
MyungSeoupChoi, YoonYoungJang, WooSeungLee, JinHoSong, YongKyooShin 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.1 8 39-45 (7 pages)
et al. (1984). The reaction mixtures in plastic microfuge tubes contained kidney homogenate (50 mg/ml), Hanks' balanced salt solution (HBSS), 75 μM ferricytochrome c, and 20 mM HEPES-tris, pH 7.4 in a total volume of 1.0 ml. After a 30 min of incubation at 37 o C, the reaction was stopped by placing the tubes in melting ice, and the tissues were rapidly pelleted by centrifuging at 1,500 g... Reactive oxygen species (ROS) have been suggested to be contributory factors in complications of diabetes mellitus. In the present study, we investigated the generation of superoxide, the lipid peroxide level measured as thiobarbituric acid reactive substances, the vasorelaxation of isolated thoracic aorta and the iNOS expression in kidney of streptozotocin induced diabetic rats. Sprague Dawley rats were divided into four groups: control, ascorbate (400 mg/kg rat weight daily in drinking water),... -
Effects of Kanagawa Hemolysin on Blood Pressure and Arterial Tone in Rats
YoungMoonKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2002, Vol.6 No.4 8 225-233 (9 pages)
C) physiological salt solution (PSS) and cleaned of connective and adipose tissues under a stereoscope. Then, the aorta was cut into rings of ∼5 mm in width. In some cases, the endothelium was removed by gentle rubbing with an angular metal rod inserted into the lumen of the ring. The completeness of functional removal of the endothelium was ascertained by absence of relaxant re- sponse to... Kanagawa hemolysin (KH), an exotoxin produced from Kanagawa phenomenon-positive Vibrio parahemolyticus, has been shown to possess various biological activities including hemolysis, enterotoxicity, cytotoxicity, and cardiotoxicity. The aim of this study was to investigate the effect of KH on the cardiovascular system and its mechanism, employing in vivo and in vitro experiments of the rat. Intracerebroventricular (icv) administration of 100 mHU KH produced a marked and continuous pressor effect... -
Nimodipine as a Potential Pharmacological Tool for Characterizing R-Type Calcium Currents
SeogBaeOh 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2001, Vol.5 No.6 8 511-519 (9 pages)
HBSS (Hank’s bal anced salt solution) (Life Technologies) for 10 ri nutes at 37"C. The digestion was halted by addition of HBSS and subsequent cenσifugation (5 rin， 1，000 rpr). The ganglia were resuspended in Har's re diur rxture F12 (Life Technologies)， supplerented with 10% FBS (fetal bovine serur) (Atlanta Biologicals， U.S.A.)， N2 supplerent (Life Technol ogies)， 50 n밍rl NGF... Nimopidine, one of dihydropyridine derivatives, has been widely used to pharmacologically identify L-type Ca currents. In this study, it was tested if nimodipine is a selective blocker for L-type Ca currents in sensory neurons and heterologous system. In mouse dorsal root ganglion neurons (DRG), low concentrations of nimodipine (＜10μM), mainly targeting L-type Ca currents, blocked high-voltage-activated calcium channel currents by ∼38%. Interestingly, high concentrations of nimodipine... -
Asymmetrical Distribution of P2Y Nucleotide Receptors in Rabbit Inner Medullary Collecting Duct Cells
JaeSukWoo, JinSupJung, YongKeunKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2000, Vol.4 No.4 6 311-318 (8 pages)
cannula placed into the aorta with Oulbeccos phosphate-buffered saline (PBS). The kidneys were then removed and rinsed with Hanks balanced salt buffer solution (HBSS) containing 50 IUjml penicillin G and 50 μgjml strep tomycin. The inner medulla was dissected， minced， suspended in HSS containing 1 mgjml collagenase， and incubated at 37"C for 2 3 h. IMCO cells were isolated by a further... We cultured the rabbit inner medullary collecting duct (IMCD) cells as monolayers on collagen-coated membrane filters, and investigated distribution of the P2Y receptors by analyzing nucleotide-induced short circuit current (Isc) responses. Exposure to different nucleotides of either the apical or basolateral surface of cell monolayers stimulated Isc. Dose-response relationship and cross-desensitization studies suggested that at least 3 distinct P2Y receptors are expressed asymmetrically on the... -
Chemical Coupling between Horizontal Cells in the Catfish Retina
Sung-JongLee, Chang-SubJung, Sun-HoBai 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.1 3 21-30 (10 pages)
oxygenated Ringer's solution consisted of 126 mM NaCI， 4 mM KCI， 3 mM CaCh， 1 mM MgCh， 15 mM dextrose， and 2 mM 5N-2-hydroxyethylpipera zine-ethanesulfonic acids-N’-2 (HEPES; Sodium Salt type)， and was titrated to pH 7.4. For studies on sodium substitution， 126 mM NaCl was replaced by equimolar HOCH2CH2N(CH3)3CI (cho1ine chloride) For studies on chloride substitution， Ringer’s...