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Visualization of Epidermis and Dermal Cells in ex vivo Human Skin Using the Confocal and Two-photon Microscopy
Choi. Sang-Hoon, Kim. Wi-Han, Lee. Yong-Joong, Lee. Ho, Lee. Weon-Ju, Yang. Jung-Dug, Shim. Jong-Won, Kim. Jin-Woong 한국광학회 Journal of the Optical Society of Korea 7 Pages
한국광학회 Journal of the Optical Society of Korea 2011, Vol.15 No.1 61-67 (7 pages)
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Toll-like Receptor 2 is Dispensable for an Immediate-early Microglial Reaction to Two-photon Laser-induced Cortical Injury In vivo
HeeraYoon, YongHoJang, SangJeongKim, SungJoongLee, SunKwangKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.5 9 461-466 (6 pages)
by microglia to detect brain tissue damage. However, its role in immedi- ate microglial reaction to brain tissue injury in vivo does not have been resolved. In the present study, we used in vivo two-photon microscopy and Cx3cr1 GFP/＋ knock-in/ ..PAGE:2 462 H Yoon, et al TLR2knock-out (KO) mice to investigate whether TLR2 plays a role in the immediate early microglial motility re- sponding to... the resting state or in immediate-early reaction to the injury in vivo. By using in vivo two-photon microscopy imaging and Cx3cr1GFP/＋ mouse line, we first monitored the motility of microglial processes (i.e. the rate of extension and retraction) in the somatosensory cortex of living TLR2-KO and WT mice; Microglial pro-cesses in TLR2-KO mice show the similar motility to that of WT mice. We further found that microglia rapidly extend their processes to the site of local tissue injury induced by... -
Detection of Near-membrane Calcium Ions in Live Tissues with a Two-Photon Fluorescent Probe
Shin. Yu-Na, Lim. Chang-Su, Tian. Yu Shun, Rho. Won-Young, Cho. Bong-Rae 대한화학회 Bulletin of the Korean Chemical Society 7 Pages
대한화학회 Bulletin of the Korean Chemical Society 2010, Vol.31 No.3 599-605 (7 pages)
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Principle of Field Emission-Scanning Electron Microscopy(FE-SEM) and its Application to the Analysis of Carbon Nanostructures
Young Jeon Kim, Chomg Rae Park 한국탄소학회 Carbon Letters 10 Pages
한국탄소학회 Carbon Letters Vol.2 No.3&4 7 202-211 (10 pages)
in a matrix. ning Electron Microscopy and X-ray Microanalysis”, 2nd ed., Plenum Press, London, 1992, Chap. 2, 3, 4. [2] Anon “Manual for Field Emission Scanning Electron Microscope”, JEOL, Japan, 2000. [3] Anon “FEG-SEM Workshop”, 16 July, Research Institute of Advanced Materials, 2001. [4] Park, C. R. Polymer Science and Technology1996,7, 75. [5] Hall, C. E. “Introduction to Electron... 최근 들어 탄소나튜브를 필두로 하는 나노 산소재에 대한 관심이 중대되면서 나노 스케일에서의 구조와 물리적 성질간의 상관성에 대한 연구가 급증하고 있다. 전자현미경은 이러한 나노 수준에서의 구조를 파악하는데 있어 없어서는 안될 기기이며 특히, 전게 방출형 주사전자형미경은 소재의 표면구조를 나노 수준까지 조사할 수 있는 아주 유효한 수다으로 알려져 있다. -
Temporal and subcellular distributions of Cy5.5-labeled hyaluronic acid nanoparticles in mouse organs during 28 days as a drug carrier
Chunmei Lin , Saet Byeol Kim , Jung-min Yon , Seul Gi Park , Lee Wha Gwon , Jong-geol Lee , In-jeoung Baek , Beom Jun Le 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 8 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2017, 제 57권 제 4호 2 215-222 (8 pages)
of HA-NPs and HA-polymers were observed based on the fluorescence intensity of Cy5.5-labeled NPs intravenously injected into mice and by transmission electron microscopy (TEM) images in cells. Materials and Methods Animals Six-week-old male ICR mice were purchased from KOAT- ECH (Korea) and allowed to acclimatize for 1 week before starting the experiment. Seven-week-old male mice weigh- ing 20 ±... -
Open Access ： Nitric Oxide and Hydrogen Peroxide Production are Involved in Systemic Drought Tolerance Induced by 2R,3R-Butanediol in Arabidopsis thaliana
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2013, 29권 4호 8 427-434 (8 pages)
as a control. All images were visualized using confocal laser scanning microscopy (excitation 488 nm, emission 515 nm; Olympus FV500). Images were ana- lyzed using SCION IMAGE software (Scion Inc., Frederick, MD, USA). Data are presented as mean pixel intensities, and 50100 guard cells were observed per treatment of three independent replicates. Detection of hydrogen peroxide production in guard... 2R,3R-Butanediol, a volatile compound produced by certain rhizobacteria, is involved in induced drought tolerance in Arabidopsis thaliana through mechanisms involving stomatal closure. In this study, we examined the involvement of nitric oxide and hydrogen peroxide in induced drought tolerance, because these are signaling agents in drought stress responses mediated by abscisic acid (ABA). Fluorescence-based assays showed that systemic nitric oxide and hydrogen peroxide production was induced by... -
Effect of Alternaria solani Exudates on Resistant and Susceptible Potato Cultivars from Two Different pathogen isolates
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 1호 3 14-19 (6 pages)
R. N. 1987. Light and scanning electron microscopy of the interaction of the biocontrol fungus Alternaria cassiae with sicklepod (Cassia obtusifolia). Plant Pathol. 9:230 Veitia, N., Dita, M. A., Garcia, L., Herrera, L., Bermudez, I., Acosta, M., Clavero, J., Orellana, P., Romero, C. and Garcia, L. 2001. Use of tissue culture and the mutagenesis in vitro for the improvement of the resistance to... Early blight of potato, caused by Alternaria solani, is a ubiquitous disease in many countries around the world. Our previous screening of several Iranian potato cultivars found that significant variation in resistance phenotypes exists between two cultivars: resistant ``Diamond`` and susceptible ``Granula``. Our previous analysis of five different pathogen isolates also identified varying degrees of aggressiveness regardless of the host cultivar. Here, a bioassay was used to study the role of... -
Synthesis of thin-multiwalled carbon nanotubes by Fe-Mo/MgO catalyst using sol-gel method
Prashant Dubey, Sang Kyu Choi, Bawl Kim, Cheol Jin Lee 한국탄소학회 Carbon Letters 10 Pages
한국탄소학회 Carbon Letters 2012, Vol.13 No.2 5 99-108 (10 pages)
by thermal decomposition of citrate precursor under various atmospheres were initially characterized by scanning electron microscopy (SEM, Hitachi S-4700), operated at an accelerat- ing voltage of 15 kV. Samples were deposited onto conductive carbon tape, which was attached to the surface of the SEM brass stub. These samples were then conductively coated with plati- num by sputtering for 20 s... -
Non-blinking dendritic crystals from C-dot solution
Ashmi Mewada, Ritesh Vishwakarma, Bhushan Patil, Chinmay Phadke 한국탄소학회 Carbon Letters 4 Pages
한국탄소학회 Carbon Letters 2015, Vol.16 No.3 8 211-214 (4 pages)
of 1 cm. Morphological characteristics of C-dots were studied using transmission electron microscopy (TEM; Carl Ziess, GmbH, Germany). The sample was loaded on a copper grid for the TEM analysis. The purifed C-dots were loaded on clean quartz plates and subjected to vacuum drying under pressure (200 mmHg) for 8 hours at 60°C (Ganesh Scientifc Ltd., India) to initiate crys- tallization. The... Bio-imaging and drug carriers for delivery have created a huge demand for crystals. Crystals are fascinating materials that have been grown for a long time but obtaining biocompatible fluorescent crystals is a challenging task. We report on the growth of fluorescent crystals using a carbon dot (C-dot) solution by a hydrothermal process. The crystallization pattern of these C-dots exhibited a unique dendritic structure having a feather-like morphology. The growth temperature and pressure were...