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Agar gel immunodiffusion analysis using baculovirus-expressed recombinant bovine leukemia virus envelope glycoprotein (gp51/gp30T-)
Seong In Lim, Wooseog Jeong, Dong Seob Tark, Dong Kun Yang, Chang Hee Kweon* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 4호 9 331-336 (6 pages)
the subtraction of OD from control well. The recombinant BLV gp51/gp30 T- was tested for its reactivity to an anti-gp51 MAb by western blotting. The anti-gp51 MAb were produced through the cell fusion method [29]. Briefly, the DNA fragment for BLV gp51/gp30 T- was ligated with pSecTaq 2C DNA vector (Invitrogen, USA) and intramuscularly inoculated to Balb/c mice. Anti- BLV gp51/gp30 T- MAbs... Bovine leukemia virus (BLV) envelope glycoprotein (gp51/ gp30T-), consisting of BLV gp51 and BLV gp30 that lacked its C-terminal transmembrane domain, was expressed in insect cells under the control of the baculovirus polyhedron promoter. Recombinant BLV gp51/gp30T- secreted from insect cells was determined by immunofluorescence, enzyme- linked immunosorbent and western blot assays using a BLV-specific monoclonal antibody and BLV-positive bovine antibodies. An agar gel immunodiffusion (AGID)... -
Multidetector row computed tomography evaluation of the micropig kidney as a potential renal donor
Woong Yoon, Min Young Lee, Jung Min Ryu, Yong Ju Moon, Sang Hun Lee, Jae Hong Park, Seung Pil Yun, Min Woo Jang, Sung Su Park, H 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 1호 2 9-13 (5 pages)
addition, multiplanar reformation using MDCT is an optimal tool for imaging complicated anatomic and pathologic cases [7]. Conventional catheter digital subtraction angiography (DSA) has been performed on potential renal donors to show the number of renal arteries and the presence of early branching and vascular disease. However, it is invasive and sometimes traumatic, needs post-procedure... ± 1.29 cm on the right side and 1.38 ± 0.17 and 5.15 ± 0.87 cm on the left side, respectively. The mean volume of the right kidney was 79.3 ± 14.5 mL and of the left kidney was 78.0 ± 13.9 mL. The data presented in this study suggest that the MDCT offers a noninvasive, rapid, and accurate method for the evaluation of the renal anatomy in living kidney donors. It also provides sufficient information about extra-renal anatomy important for donor surgery and determination of organ suitability. -
Antinociceptive Effect of Cyperi rhizoma and Corydalis tuber Extracts on Neuropathic Pain in Rats
Jae-GyunChoi, Suk-YunKang, Jae-MinKim, Dae-HyunRoh, Seo-YeonYoon, JinBongPark, Jang-HernLee, Hyun-WooKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2012, Vol.16 No.6 3 387-392 (6 pages)
drug. Lines in each panel indicate the treatment period of CT or pregabalin. *p ＜0.05 and **p＜0.01 control vs CR. #p＜0.05 and ##p＜0.01 control vs prega- balin. of each image after background subtraction and shading correction. The microscope illumination and data acquis- ition settings were fixed throughout the entire analysis procedure. The average number of pNR1-ir neurons per sec- ti... significantly decreased the nerve injury-induced mechanical allodynia. In addition, CR and CT at doses of 10 or 30 mg/kg alleviated thermal heat hyperalgesia when they were treated in the maintenance period. Finally, CR or CT (30 mg/kg) treated during the induction period remarkably reduced the nerve injury-induced phosphorylation of NMDA receptor NR1 subunit (pNR1) in the spinal dorsal horn. Results of this study suggest that extracts from CR and CT may be useful to alleviate neuropathic pain. -
Higenamine이 심근 Sarcoplasmic Reticulum의 칼슘운반에 미치는 영향에 관한 연구
김주현(Joo Hyun Kim), 이영균(Yung Kyoon Lee), 김혜원(Hae Won Kim), 김명석(Myung Suk Kim), 박찬웅(Chan Woong Park), 임정규(Jung Kyoo Lim) 대한약리학회 대한약리학잡지 10 Pages
대한약리학회 대한약리학잡지 1982, 제 18권 제 2호 9 79-88 (10 pages)
EGTA (for Mg++-ATPase). After the preincubation the reaction was started by' the addition of 1 mM Tris-ATP. The reaction was terminated by the addition of O. 25 ml of 15% TCA at 5 min after the initiation of the reaction and Pi liberated was determined spectrophotometrically by the method of Horwitt. Ca++-ATPase activity was calculated by the subtraction of Mg++-ATPase from the total ATPase.... -
Effects of In Vitro Exposure to Silica on Bioactive Mediator Release by Alveolar Macrophages
Lee, Ji-Hee 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1995, 제 29권 제 1호 1 1-12 (12 pages)
x 100. All test values were corrected for resting serotonin release by subtraction of dPM values from control samples containing only cells and buffer. Release values then were calculated as percentage of total cellular serotonin and converted to nmoles of P AF released using P AF standard curves. Measurement of the release of IL-1 Alveolar macrophage cultures and supernate preparation:... Alveolar macrophages play a pivotal role in the pathogenesis of silicosis since the macrophages may release a wide variety of toxic and inflammatory mediators as well as mitogenic growth factors. In the present study, the effects of in vitro exposure to silica on release of various mediator such as reactive oxygen species, platelet activating factor(PAF), and interleukin-1 (IL-1) by alveolar macrophages were examined. First, hydrogen peroxide release from alveolar macrophages was monitored by... -
Identification of Differentially Up-regulated Genes in Apple with White Rot Disease
Yeo-Jin Kang, Young Koung Lee, In-Jung Kim 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 5호 13 530-537 (8 pages)
genes involved in specific functions and genes with differential expression patterns (Dougherty et al., 2018). Methods for identifying genes induced by patho- gen infection include suppression subtraction hybridization (Gao et al., 2012), cDNA microarray (Okomo-Adhiambo et al., 2006), and differential display reverse transcription polymerase chain reaction (DDRT-PCR) (Singh et al., 2013),... Fuji, a major apple cultivar in Korea, is susceptible to white rot. Apple white rot disease appears on the stem and fruit; the development of which deteriorates fruit quality, resulting in decreases in farmers income. Thus, it is necessary to characterize molecular markers related to apple white rot resistance. In this study, we screened for differentially expressed genes between uninfected apple fruits and those infected with Botryosphaeria dothidea, the fungal pathogen that causes white rot.... -
Measuring Teacher Knowledge
Kim Rae-Young, 김래영, Peter Youngs, 피터 영스 한국교육행정학회 교육행정학연구 20 Pages
한국교육행정학회 교육행정학연구 2009, 제 27권 제 3호 22 497-516 (20 pages)
to investigate and solve mathematical problems Code Themes SC1 Curriculum components SC2 Prenumber and number concepts SC3 Baseten numeration system SC4 Addition and subtraction of whole numbers SC5 Multiplication and division SC6 Concepts related to number theory APPENDIX II: Objectives of mathematical content knowledge in teacher certification tests 1. MTTC test (Elementary Education test) 2.... -
Early Mathematical Learning: Number Processing Skills and Executive Function at 5 and 8 Years of Age
Sonia L. J. White, Donna Berthelsen, Sue Walker, Kate E. Williams 환태평양유아교육연구학회 Asia-Pacific journal of research in early childhood education 22 Pages
환태평양유아교육연구학회 Asia-Pacific journal of research in early childhood education 2015, Vol.9 No.3 4 45-66 (22 pages)
two-minute period. Reaction time and accuracy were recorded; with the correct number of responses per second the final score. Calculation. involved single digit addition and subtraction (e.g., 2 + 1, 5 1). Children provided a verbal response which was recorded. Each stimulus (e.g., 4 + 3) was presented until a response was given. Similar to the Test for Early Mathematics Ability (Ginsburg &... This research investigated differences and associations in performance in number processing and executive function for children attending primary school in a large Australian metropolitan city. In a cross-sectional study, performance of 25 children in the first full-time year of school, (Prep; mean age = 5.5 years) and 21 children in Year 3 (mean age = 8.5 years) completed three number processing tasks and three executive function tasks. Year 3 children consistently outperformed the Prep year... -
교과 교육과정의 성취기준 개발 실태와 개선 방안 탐색
이승미, 박순경 한국교육학회 교육학연구 27 Pages
한국교육학회 교육학연구 2014, 52권 2호 3 53-79 (27 pages)
: Use addition and subtraction within 100 to solve word problems involving lengths that are given in the same units,e.g.,by using drawings (such as drawings of rulers) and equations with a symbol for the unknown number to represent the problem. 영국의 초등학교 교육과정에서는 미국가 형식을 달리하되 유사한 방식으로 내용을 상세화하 ..PAGE:13 교과 교육과정의 성취기준 개발 실태와 개선 방안 탐색 65 고... 본 연구에서는 2009 개정 교육과정에 따른 교과 교육과정에서 ‘성취기준’의 실태와 문제점을 분석 하고 외국 교육과정 문서 분석과 델파이 조사 결과에 기반하여 그에 따른 개선 방안을 마련하고자 하 였다. 연구 결과, 본 연구에서는 교과 교육과정 문서의 내부적인 측면으로 ‘성취기준’이라는 용어가 남 용되고 있으며 학습 내용 성취기준에서 ‘내용’과 ‘수행’의 기술 방식이 형식적으로 고정화되어 있는 문 제를 제기하였고, 교과 교육과정 문서의 외부적인 측면으로 평가기준으로서의 성취기준이 국가 교육과 정 문서와는... -
Preparation and characterization of chemically activated carbon materials for CO₂ capture
Da-Hee Jeon, Shin-Tae Bae, Soo-Jin Park 한국탄소학회 Carbon Letters 5 Pages
한국탄소학회 Carbon Letters 2016, Vol.17 No.1 13 85-89 (5 pages)
: mesopore volume determined from the subtraction of mesopore volume from total pore volume. e Fmicro : (micropore volume/total pore volume) × 100 Fig. 1. N2 full isotherms at 77 K porosity parameters of the Pristine-AC and K-AC samples Fig. 2. Meso-(a) and micro-(b) pore size distribution of the Pristine-AC and K-AC samples ..PAGE:3 Chemically activated carbon materials for CO2 capture 87...