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Comparison of the age-related porcine endogenous retrovirus (PERV) expression using duplex RT-PCR
Hyoung Joon Moon, Hye Kwon Kim, Seong Jun Park, Chul Seung Lee, Dae Sub Song, Bo Kyu Kang, Bong Kyun Park* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 4호 7 317-322 (6 pages)
alternative source for xenotransplantation. Sensitive duplex reverse transcription-polymerase chain reaction protocols for simultaneously detecting PERV gag mRNA and porcine glyceraldehydes 3-phosphate dehydrogenase mRNA in one tube was established. To compare the age-related PERV expression patterns of the lung, liver, spleen, kidney, heart, and pancreas in commercial pigs, 20 pigs from four age groups (5 heads each in 10 days-, 40 days-, 70 days-, and 110 days-old, respectively) were used in... -
A Duplex PCR Assay for Rapid Detection of Phytophthora nicotianae and Thielaviopsis basicola
Na Liu, Shijun Jiang, Songli Feng, Wenyan Shang, Guozhen Xing, Rui Qiu, Chengjun Li, Shujun Li, Wenming Zheng 한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 2호 9 172-177 (6 pages)
A duplex PCR method was developed for simultaneous detection and identification of tobacco root rot pathogens Phytophthora nicotianae and Thielaviopsis basicola. The specific primers for P. nicotianae were developed based on its internal transcribed spacer (ITS) regions of ribosomal gene, ras gene and hgd gene, while the specific primers for T. basicola were designed based on its ITS regions and β-tubulin gene. The specificity of the primers was determined using isolates of P. nicotianae, T.... -
Detection of Escherichia coli O157:H7, Listeria monocytogenes, Salmonella spp. and Staphylococcus aureus using duplex real-time PCR assay with melting curve analysis on fresh lettuce
Lee. Na-Ri, Kwon. Kyung-Yoon, Choi. Sung-Wook, Koo. Min-Seon, Chun. Hyang-Sook 한국식품위생안전성학회 한국식품위생안전성학회지 6 Pages
한국식품위생안전성학회 한국식품위생안전성학회지 2011, Vol.26 No.2 114-119 (6 pages)
황색포도상구균의 thermonuclease (nuc), 리스테리아의 hemolycin (hly), 살모넬라의 tetrahionate reductase (ttr) 를 특이적으로 검출할 수 있는 4종의 프라이머 세트에 대한 real-time PCR의 melting curve 분석을 통하여 황색포도상구균과 대장균 동시분석 시 $T_m$ 값이 $80.6{pm}0.9^{circ}C$와 $86.9{pm}0.5^{circ}C$, 리스테리아와 살모넬라 동시분석 시 Tm 값이 $80.4{pm}0.6^{circ}C$와 $88.1{pm}0.11^{circ}C$로 확인하였고, 그 결과 정제되어진 각 식중독균의 genomic DNA를 주형으로 한 duplex real-time PCR 방법이 uniplex... -
Duplex PCR을 이용한 국내 미승인 유전자변형 감자(EH92-527-1)의 검사법 개발
유명렬, 김재환, 예미지, 김해영, Yoo. Myung-Ryul, Kim. Jae-Hwan, Yea. Mi-Chi, Kim. Hae-Yeong 한국식품과학회 한국식품과학회지 5 Pages
한국식품과학회 한국식품과학회지 2013, Vol.45 No.2 156-160 (5 pages)
유전자변형 감자 EH92-527-1를 검출하기 위한 duplex PCR 검사법이 개발되었다. 감자의 내재유전자로 UDP-glucose pyrophosphorylase (UGP)가 선별되었고, 14개 다른 작물을 이용하여 특이성이 확인되었다. 유전자변형 감자에 삽입된 T-DNA 영역과 감자 게놈 사이의 연결 부위를 증폭하도록 프라이머 EH92-F/R 쌍이 제작되었고, 몇 개의 다른 유전자 변형 작물을 이용하여 특이성이 확인되었다. 서론에서 언급한 바와 같이 BASF사에서 각 개발된 유전자변형 감자 EH92-527-1과 BPS-A1020-5가 GBSS 유전자를 동일하게 포함하고 있으나 본... -
Species-Specific Duplex PCR for Detecting the Important Fish Pathogens Vibrio anguillarum and Edwardsiella tarda
Jo. Geon-A, Kwon. Sae-Bom, Kim. Na-Kyeong, Hossain. Muhammad Tofazzal, Kim. Yu-Ri, Kim. Eun-Young, Kong. In-Soo 한국수산과학회 Fisheries and aquatic sciences 5 Pages
한국수산과학회 Fisheries and aquatic sciences 2013, Vol.16 No.4 273-277 (5 pages)
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붉은사슴과 엘크에서 SRY와 ZFX-ZFY 유전자의 Duplex PCR기법을 이용한 성 판별
한상현, 이성수, 고문석, 조인철, Han. S.H., Lee. S.S., Ko. M.S., Cho. I.C. 한국동물자원과학회 한국동물자원과학회지 8 Pages
한국동물자원과학회 한국동물자원과학회지 2007, Vol.49 No.1 1-8 (8 pages)
일종인 bovine SINE element와 유사한 서열이 관찰되었다. SRY와 ZFX-ZFY 유전자들을 동시에 증폭하는 duplex PCR을 통해 유전자 성 판별을 수행하였고, 암수가 서로 구분되는 증폭 양상을 나타내었다: 암컷에서는 ZFX에서 증폭된 공통의 증폭 산물 하나만이 관찰되었고 수컷은 세 개의 밴드가 관찰되었다(ZFX에 해당하는 공통의 밴드와 ZFY와 SRY에서 증폭된 두 개의 수컷 특이 밴드). 두 가지 유전자에 대한 독립적인 PCR 시험에서 얻은 결과는 duplex PCR에 의해 얻은 결과와 동일한 양상을 나타내었다. 또한 유전자 성 판별의 결과들... -
Survey of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus incidence in Korea by Duplex RT-PCR
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2014, 30권 4호 14 445-449 (5 pages)
a newly developed duplex reverse transcriptase polymerase chain reaction (RT-PCR) method that can detect both viruses in a single reaction. CNRMV and CGRMV co-infection rates were 29.6%, 53.6%, and 17.6%, respectively, in samples collected from three different sites (Daegu, Gyeongju and Gyeongsan) in Gyeongbuk province during 2012 and 2013. This duplex RT-PCR method offers a simple, rapid, and effective way of identifying CNRMV and CGRMV simultaneously in sweet cherry trees, which can aid in the... -
Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples
Hye Kwon Kim, Kwang Soo Lyoo, Thi My Le Huynh, Hyoung Joon Moon, Van Giap Nguyen, Bong Kyun Park 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 16 253-256 (4 pages)
duplex nested reverse transcriptase polymerase chain reaction (RT-PCR) method that targets the genomic RNA of type 2 PRRSV and the mRNA of PCV2 in infected tissues. The method amplified discriminative bands of 347 bp and 265 bp specific for type 2 PRRSV and PCV2, respectively. The limits of detection of the duplex nested RT-PCR were 10 TCID50/mL for type 2 PRRSV and 10 infected cells/mL for PCV2. The kappa statistic, which measures agreement between methods, was 0.867, indicating a good level of... -
Detection of Streptococcus dysgalactiae subsp. equisimilis in equine nasopharyngeal swabs by PCR
Silvia Preziuso, *, Fulvio Laus, Aurora Romero Tejeda, Carlo Valente, Vincenzo Cuteri2 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 1호 10 67-72 (6 pages)
been isolated from cases of strangles-like disease. Rapid and sensitive diagnostic techniques could enable epidemiological studies and effective investigation of outbreaks involving these bacteria. In this study, PCR protocols previously described in cattle and in humans to detect the species S. dysgalactiae and the subspecies equisimilis were evaluated to detect specific sequences in equine samples. For this purpose, 99 monolateral nasal swabs were collected from horses from stud farms with a... -
Development of a Multiplex PCR Method to Detect Fungal Pathogens for Quarantine on Exported Cacti
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 1호 7 53-57 (5 pages)
spp. are known as causal pathogens. These pathogens can lead to plant death after infection. Therefore, some European countries have quarantined imported cacti that are infected with specific fungal pathogens. Consequently, we developed PCR detection methods to identify four quarantined fungal pathogens and reduce export rejection rates of Korean grafted cacti. The pathogen specific primer sets F.oF-F.oR, B.CF-B.CR, P.nF-P.nR, and P.cF-P.CR were tested for F. oxysporum, B. cactivora, P.... -
Development and Practical Use of RT-PCR for Seed-transmitted Prune dwarf virus in Quarantine
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2014, 30권 2호 8 178-182 (5 pages)
In this study, two RT-PCR primer sets, which can promptly and specifically diagnose plant quarantine seed-transmitted PDV, were developed; and nested PCR primers, where products amplify 739 and 673 nucleotides (nt), and an nested PCR-product, 305 nt, can be obtained as these products are amplified again, were developed. Also, a modified-positive control plasmid was developed, where the restriction enzyme XhoI, which can identify the contamination of samples from the control, was inserted. The... -
Research Notes : Multiplex PCR Assay for Simultaneous Detection of Korean Quarantine Phytoplasmas
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 4호 10 367-371 (5 pages)
Multiplex PCR assays were developed for the simultaneous detection of ten important Korean quarantine phytoplasmas. The species-specific primers were designed based on ribosomal protein, putative preprotein translocase Y, immunodominant protein, elongation factor TU, chaperonin protein and the 16S rRNA genes of ``Candidatus (Ca.) Phytoplasma`` species. Three main primer sets were prepared from ten designed primer pairs to limit nonspecific amplification as much as possible. The multiplex PCR... -
Development of a Multiplex RT-PCR for the Simultaneous Detection of Three Viruses in Cherry Plants
Chung Youl Park, Jeong-ran Park, Geun-sik Lee, Seung-In Yi, Byeong Hoon Kim, Jung Sik Eom, Som Gyeol Lee, Hong-sup Kim 한국식물병리학회 식물병연구 4 Pages
한국식물병리학회 식물병연구 2018, 24권 3호 7 233-236 (4 pages)
A multiplex RT-PCR (mRT-PCR) assay was developed for the detection of the recently reported viruses, Cherry virus A (CVA), Little cherry virus 1 (LChV-1), and Little cherry virus 2 (LChV-2), in cherry plants in Korea. Eight sets of primers were designed for each virus and their specificity was tested by using various combinations of mixed primer sets. From the designed primer sets, one combination was selected and further evaluated to estimate the optimum temperature and detection limits of the...
