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전체 선택해제
총 471건 검색
본문검색 결과만 보기
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Development of Useful Products Through Plant Cell Fusion and Culture of Populus spp.(II)
김길웅, 박용구, 최명석, Kim. K.U., Park. Y.G., Choi. M.S. 한국잡초학회 한국잡초학회지 6 Pages
한국잡초학회 한국잡초학회지 1995, Vol.15 No.2 160-165 (6 pages)
현사시나무의 조직 배양 세포에서의 anthocyanin 생산성이 높은 세포계를 얻기 위한 목적으로 실험을 수행한 바 다음과 같은 결과를 얻었다. Anthocyanin 생산력이 높은 세포계의 선발은 sucrose 3%, 2, 4-D 0.5 mg/l, BAP 0.1 mg/l가 첨가된 MS 기본배치에서 실시하여 15 개 cell line중에 생성력이 80%에 달한 ACL 11 세포계를 선발하였다. Anthocyanin 생성에는 배지 환경요소가 중요한 인자로 작용함을 밝혀 냈다. -
Quantitative Screening of Insect Cell Transformants Stably Expressing $GFP_{uv}-{eta}1$, 3-N-acetylglucosaminyltransferase 2 Fusion Protein
Deo. Vipin Kumar, Kato. Tatsuya, Asari. Naoko, Park. Enoch Y. 한국생물공학회 Biotechnology and bioprocess engineering 5 Pages
한국생물공학회 Biotechnology and bioprocess engineering 2005, Vol.10 No.3 275-279 (5 pages)
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본고에서는 kem1 유전자의 기능에 대해 중점적으로 연구한 결과를 서술하려 한다.
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Defining the N-Linked Glycosylation Site of Hantaan Virus Envelope Glycoproteins Essential for Cell Fusion
Zheng. Feng, Ma. Lixian, Shao. Lihua, Wang. Gang, Chen. Fengzhe, Zhang. Ying, Yang. Song 한국미생물학회 The journal of microbiology 1 Pages
한국미생물학회 The journal of microbiology 2008, Vol.46 No.2 233-233 (1 pages)
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Defining the N-Linked Glycosylation Site of Hantaan Virus Envelope Glycoproteins Essential for Cell Fusion
Zheng. Feng, Ma. Lixian, Shao. Lihua, Wang. Gang, Chen. Fengzhe, Zhang. Ying, Yang. Song 한국미생물학회 The journal of microbiology 7 Pages
한국미생물학회 The journal of microbiology 2007, Vol.45 No.1 41-47 (7 pages)
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Production of Recombinant Polyhedra Containing Cry1Ac Fusion Protein in Insect Cell Lines
Kim. Jae-Su, Choi. Jae-Young, Roh. Jong-Yul, Lee. Han-Young, Jang. Seung-Sik, Je. Yeon-Ho 한국미생물생명공학회 Journal of microbiology and biotechnology 6 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2007, Vol.17 No.5 739-744 (6 pages)
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Identification of a Novel Fusion Gene (HLA-E and HLA-B) by RNA-seq Analysis in Esophageal Squamous Cell Carcinoma
Jiang. Yu-Zhang, Li. Qian-Hui, Zhao. Jian-Qiang, Lv. Jun-Ji 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP 4 Pages
아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP 2014, Vol.15 No.5 2309-2312 (4 pages)
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Asparagus Racemosus Leaf Extract Inhibits Growth of UOK 146 Renal Cell Carcinoma Cell Line: Simultaneous Oncogenic PRCCTFE3 Fusion Transcript Inhibition and Apoptosis Independent Cell Death
Verma. Shiv Prakash, Tripathi. Vikash Chandra, Das. Parimal 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP 5 Pages
아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP 2014, Vol.15 No.5 1937-1941 (5 pages)
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Cytotoxic T Lymphocytes Elicited by Dendritic Cell-Targeted Delivery of Human Papillomavirus Type-16 E6/E7 Fusion Gene Exert Lethal Effects on CaSki Cells
Wu. Xiang-Mei, Liu. Xing, Jiao. Qing-Fang, Fu. Shao-Yue, Bu. You-Quan, Song. Fang-Zhou, Yi. Fa-Ping 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP 5 Pages
아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP 2014, Vol.15 No.6 2447-2451 (5 pages)
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Interactions among Measles Virus Hemagglutinin, Fusion Protein and Cell Receptor Signaling Lymphocyte Activation Molecule (SLAM) Indicating a New Fusion-trimer Model
Zhang. Peng, Li. Lingyun, Hu. Chunlin, Xu. Qin, Liu. Xin, Qi. Yipeng 생화학분자생물학회 Journal of biochemistry and molecular biology 8 Pages
생화학분자생물학회 Journal of biochemistry and molecular biology 2005, Vol.38 No.4 373-380 (8 pages)
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Detection of the cell wall-affecting antibiotics at sublethal concentrations using a reporter Staphylococcus aureus harboring drp35 promoter - lacZ transcriptional fusion
Mondal. Rajkrishna, Chanda. Palas K., Bandhu. Amitava, Jana. Biswanath, Lee. Chia-Y., Sau. Subrata 생화학분자생물학회 BMB reports 6 Pages
생화학분자생물학회 BMB reports 2010, Vol.43 No.7 468-473 (6 pages)
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Imaging Findings of Renal Cell Carcinoma Associated with Xp11.2 Translocation/TFE3 Gene Fusion in a 4-Year-Old Male: Case Report and Review of Literature
김현지, 이미정, 이사라, 김명준, 홍창희, Kim. Hyun Gi, Lee. Mi-Jung, Lee. Sarah, Kim. Myung-Joon, Hong. Chang Hee 대한자기공명의과학회 대한자기공명의과학회지 6 Pages
대한자기공명의과학회 대한자기공명의과학회지 2013, Vol.17 No.1 41-46 (6 pages)
신세포암의 여러 종류 중 Xp11.2 염색체 재배열/TFE3 유전자 융합 연관 신세포암은 드물며 소아나 젊은 성인에서 더 흔한 것으로 알려져 있다. 이 증례보고는 병리학적으로 확인된 4세 남자 환자의 Xp11.2 염색체 재배열/TFE3 유전자 융합 연관 신세포암에 대한 것이다. 본 증례에서 종양은 컴퓨터 단층 촬영에서 우측 신장에 2.5 cm 크기의 경계가 불명확한 종괴로 보였으며, 초음파상 고에코 병변으로 보였다. 자기공명영상에서는 종괴 캡슐의 조영증강과 함께 종괴의 확산 제한이 보였다. 저자들은 이 드문 신세포암의 영상 소견에... -
Vaccination with an Ovalbumin/Interleukin-4 Fusion DNA Efficiently Induces Th2 Cell-Mediated Immune Responses in an Ovalbumin-Specific Manner
Lim. Young-Shin, Kang. Bok-Yun, Kim. Eui-Jin, Kim. Seung-Hyun, Hwang. Seung-Yong, Kim. Kyeong-Man, Kim. Tae-Sung 대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 6 Pages
대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 1998, Vol.21 No.5 537-542 (6 pages)
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Sequential Changes of Plasma C-Reactive Protein, Erythrocyte Sedimentation Rate and White Blood Cell Count in Spine Surgery : Comparison between Lumbar Open Discectomy and Posterior Lumbar Interbody Fusion
Choi. Man Kyu, Kim. Sung Bum, Kim. Kee D., Ament. Jared D. 대한신경외과학회 Journal of Korean neurosurgical society 6 Pages
대한신경외과학회 Journal of Korean neurosurgical society 2014, Vol.56 No.3 218-223 (6 pages)
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Enhanced Induction of T Cell Immunity Using Dendritic Cells Pulsed with HIV Tat and HCMV-pp65 Fusion Protein In Vitro
Park. Jung-Sun, Park. Soo-Young, Cho. Hyun-Il, Sohn. Hyun-Jung, Kim. Tai-Gyu 대한면역학회 Immune network : official journal of the Korean association of immunobiologists 8 Pages
대한면역학회 Immune network : official journal of the Korean association of immunobiologists 2011, Vol.11 No.3 182-189 (8 pages)
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Virus-cell Fusion Inhibitory Activity for the Polysaccharides from Various Korean Edible Clams
Woo. Eun-Rhan, Kim. Wan-Seok, Kim. Yeong-Shik 대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 4 Pages
대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 2001, Vol.24 No.6 514-517 (4 pages)
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저근백피의 Virus-Cell Fusion 저해활성 성분
장영수, 문영희, 우은란, Chang. Young-Su, Moon. Young-Hee, Woo. Eun-Rhan 한국생약학회 생약학회지 5 Pages
한국생약학회 생약학회지 2003, Vol.34 No.1 28-32 (5 pages)
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Establishment and identification of cell lines from type O blood Korean native pigs and their efficiency in supporting embryonic development via somatic cell nuclear transfer
Anukul Taweechaipaisankul, Geon A Kim, Jun-Xue Jin, Su Cheong Yeom, Byeong Chun Lee 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 4호 3 492-499 (8 pages)
of blood type O, genomic DNA, fucosyltransferase (FUT) expression, and immunofluorescence staining were examined. Additionally, fluorescence-activated cell sorting and somatic cell nuclear transfer were performed to investigate the normality of the cell lines and to evaluate their effectiveness in embryo development. We found no significant bands corresponding to specific blood group A, and no increase in FUT expression in cell lines derived from piglets No. 1, No. 4, No. 5, No. 8, and the adult... -
Improved development of somatic cell cloned bovine embryos by a mammary gland epithelia cells in vitro model
Xiao-ying He, Li-bing Ma, Xiao-ning He, Wan-tong Si, Yue-Mao Zheng 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 3 145-152 (8 pages)
cells in vitro model by the adenovirus-mediated telomerase (hTERT-bMGEs). The present study was conducted to confirm whether hTERT-bMGEs were effective target cells to improve the efficiency of transgenic expression and somatic cell nuclear transfer (SCNT). To accomplish this, a mammary-specific vector encoding human lysozyme and green fluorescent protein was used to verify the transgenic efficiency of hTERT-bMGEs, and untreated bovine mammary gland epithelial cells (bMGEs) were used as a... -
Improved immunogenicity of Newcastle disease virus inactivated vaccine following DNA vaccination using Newcastle disease virus hemagglutinin-neuraminidase and fusion protein genes
Masoumeh Firouzamandi, Hassan Moeini, Davood Hosseini, Mohd Hair Bejo, Abdul Rahman Omar, Parvaneh Mehrbod, Aini Ideris 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 3 21-26 (6 pages)
The present study describes the development of DNA vaccines using the hemagglutinin-neuraminidase (HN) and fusion (F) genes from AF2240 Newcastle disease virus strain, namely pIRES/HN, pIRES/F and pIRES-F/HN. Transient expression analysis of the constructs in Vero cells revealed the successful expression of gene inserts in vitro. Moreover, in vivo experiments showed that single vaccination with the constructed plasmid DNA (pDNA) followed by a boost with inactivated vaccine induced a significant... -
Rapamycin treatment during in vitro maturation of oocytes improves embryonic development after parthenogenesis and somatic cell nuclear transfer in pigs
Joohyeong Lee, Jong-Im Park, Jung Im Yun, Yongjin Lee, Hwanyul Yong, Seung Tae Lee, Choon-Keun Park, Sang-Hwan Hyun, Geun-Shik L 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 16 373-380 (8 pages)
This study was conducted to investigate the effects of rapamycin treatment during in vitro maturation (IVM) on oocyte maturation and embryonic development after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) in pigs. Morphologically good (MGCOCs) and poor oocytes (MPCOCs) were untreated or treated with 1 nM rapamycin during 0–22 h, 22–42 h, or 0–42 h of IVM. Rapamycin had no significant effects on nuclear maturation and blastocyst formation... -
In vitro development of canine somatic cell nuclear transfer embryos in different culture media
Dong-Hoon Kim, Jin-Gu No, Mi-Kyung Choi, Dong-Hyeon Yeom, Dong-Kyo Kim, Byoung-Chul Yang, Jae Gyu Yoo, Min Kyu Kim, Hong-Tea Kim 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 14 233-235 (3 pages)
of canine somatic cell nuclear transfer (SCNT) embryos. Canine cloned embryos were cultured in modified synthetic oviductal fluid (mSOF), porcine zygote medium-3 (PZM-3), or G1/G2 sequential media. Our results showed that the G1/G2 media yielded significantly higher morula and blastocyst development in canine SCNT embryos (26.1% and 7.8%, respectively) compared to PZM-3 (8.5% and 0%) or mSOF (2.3% and 0%) media. In conclusion, this study suggests that blastocysts can be produced more efficiently... -
Identification of abnormal gene expression in bovine transgenic somatic cell nuclear transfer embryos
Jongki Cho, Sungkeun Kang, Byeong Chun Lee* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 8 225-231 (7 pages)
This study was conducted to investigate the expression of three genes related to early embryonic development in bovine transgenic cloned embryos. To accomplish this, development of bovine transgenic somatic cell nuclear transfer (SCNT) embryos was compared with non-transgenic embryos. Next, mRNA transcription of three specific genes (DNMT1, Hsp 70.1, and Mash2) related to early embryo development in transgenic SCNT embryos was compared between transgenic and non-transgenic SCNTs, parthenogenetic... -
Mitochondrial and DNA damage in bovine somatic cell nuclear transfer embryos
In-Sun Hwang, Hyo-Kyung Bae, Hee-Tae Cheong 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 1 235-240 (6 pages)
bovine somatic cell nuclear transfer (SCNT) embryos were examined. Bovine enucleated oocytes were electrofused with donor cells and then activated by a combination of Ca-ionophore and 6-dimethylaminopurine culture. The H2O2 and ·OH radical levels, mitochondrial morphology and membrane potential (ΔΨ), and DNA fragmentation of SCNT and in vitro fertilized (IVF) embryos at the zygote stage were analyzed. The H2O2 (35.6 ± 1.1 pixels/embryo) and ·OH radical levels (44.6 ± 1.2 pixels/embryo) of... -
Aerosol delivery of kinase-deficient Akt1 attenuates Clara cell injury induced by naphthalene in the lungs of dual luciferase mice
Arash Minai-Tehrani, Young-Chan Park, Soon-Kyung Hwang, Jung-Taek Kwon, Seung-Hee Chang, Sung-Jin Park, Kyeong-Nam Yu, Ji-Eun Ki 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 2 309-317 (9 pages)
KD did not. Our results showed that naphthalene affected extracellular signal-regulated kinase (ERK) protein levels, ERK-related signaling, and induced Clara cell injury. However, Clara cell injury induced by naphthalene was considerably attenuated in mice exposed to Akt1 KD. Furthermore, a dual luciferase activity assay showed that aerosol delivery of Akt1 WT and naphthalene treatment enhanced cap-dependent protein translation, while reduced cap-dependent protein translation was observed after... -
Cloned calves derived from somatic cell nuclear transfer embryos cultured in chemically defined medium or modified synthetic oviduct fluid
Goo Jang*, So Gun Hong, Byeong Chun Lee* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 12 83-89 (7 pages)
Somatic cell nuclear transfer (SCNT) is considered to be a critical tool for propagating valuable animals. To determine the productivity calves resulting from embryos derived with different culture media, enucleated oocytes matured in vitro were reconstructed with fetal fibroblasts, fused, and activated. The cloned embryos were cultured in modified synthetic oviduct fluid (mSOF) or a chemically defined medium (CDM) and developmental competence was monitored. After 7 days of culturing, the... -
Antigenic and immunogenic investigation of the virulence motif of the Newcastle disease virus fusion protein
Kang-Seuk Choi*, Eun-Kyoung Lee, Woo-Jin Jeon, Jun-Hun Kwon 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 5 205-211 (7 pages)
have a multibasic amino acid sequence (virulence motif) such as RRQKRF at the cleavage site of the precusor fusion (F0) protein. The antigenic and immunogenic characteristics of the virulence motif RRQKRF in the F0 protein of virulent NDVs were investigated. Epitope mapping analysis revealed that a RRQKRF-specific monoclonal antibody 4G2 recognized the KRF section of the motif. A synthetic peptide bearing the RRQKRF motif reacted strongly with sera from virulent NDV (with RRQKRF motif)-infected...
