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Electron Microscopic Grid를 이용한 초급속동결이 소 난포란의 발달능에 미치는 영향 - I. 소 미성숙난자의 동결에 관한 연구
김은영, 김남형, 이봉경, 윤산현, 박세필, 정길생, 임진호, Kim. E.Y., Kim. N.H., Yi. B.K., Yoon. S.H., Park. S.P., Chung. K.S., Lim. J.H. 대한생식의학회 大韓不妊學會雜誌 6 Pages
대한생식의학회 大韓不妊學會雜誌 1998, Vol.25 No.1 71-76 (6 pages)
소 미성숙난자를 electron microscope (EM) grid와 동해제인 EFS30을 이용하여 초급속 동결하였을 때 정상적인 배 발달의 유도가능성 여부를 조사하고자 실시하였다. 동해제는 30% ethylene glycol, 18% ficoll, 0.5 M sucrose와 10% FBS 등이 PBS에 첨가되어 제작된 EFS30을 사용하였다. 난자 생존의 평가기준으로는 성숙, 수정 및 배발달을 조사하였다. 본 연구에서 얻어진 결과는 다음과 같다. 초급속동결-융해 후, 소 미성숙란의 생존율은 43.2%을 나타내었다. 동결-융해군의 체외 성숙 (84.1%)과 정상 자웅전핵 형성율 (57.5%)은... -
Helicobacter apodemus sp. nov., a new Helicobacter species identified from the gastrointestinal tract of striped field mice in Korea
Woo Jin Jeon, Hee-Jin Dong, Jae Hoon Shin, Il Yong Kim, Hungwui Ho, Seung Hyun Oh, Young Min Yoon, Yang-Kyu Choi, Jun Gyo Suh, K 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 11 475-481 (7 pages)
to cephalothin and nalidixic acid, and weakly positive for oxidase and catalase activity. Electron microscopy revealed that the bacterium has spirally curved rod morphology with singular bipolar nonsheathed flagella. Genotypically, the isolated bacterial strains (YMRC 000215, YMRC 000216, and YMRC 000419) were most closely related to a reference strain of Helicobacter mesocricetorum (97.25%, 97.32%, and 97.03% 16S rRNA sequence similarities, respectively). The 16S rRNA sequences of these strains... -
The expression of plasmid mediated afimbrial adhesin genes in an avian septicemic Escherichia coli strain
Eliana Guedes Stehling, *, Tatiana Amabile Campos, Marcelo Brocchi, Vasco Ariston de Carvalho Azevedo, Wanderley Dias da Silveira 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 10 75-83 (9 pages)
electron microscopy), and had five plasmids of 2.7, 4.7, 43, 56, and 88 MDa. Transposon mutagenesis of strain SEPT13, with transposon TnphoA, resulted in a mutant strain named ST16 that had a LD50 of 1.2 × 1012 CFU/ml. All other biological characteristics of strain ST16 were the same as those detected for strain SEPT13 except for the migration of an 88 MDa plasmid to the 93 MDa position indicating the insertion of the transposon into the 88 MDa plasmid. The 93 MDa plasmid of strain ST16 was... -
Escherichia coli O157:H7 adherence to HEp-2 cells is implicated with curli expression and outer membrane integrity
Sang-Hyun Kim, Yong-Hwan Kim 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 5 119-124 (6 pages)
C. A few CR-binding negative (CR-) colonies occurred spontaneously within the population of CR+ isolate. The CR+ EHEC showed massive aggregative adhesion pattern, whereas the spontaneous CR- strain showed typical localized adherence on HEp-2 cells. Electron microscopy confirmed highly curliated bacteria in the CR+ EHEC sample. Interestingly, the curliation disappeared in a msbB1 and msbB2 double mutant derived from the CR+ EHEC. These results suggest that the compromised outer membrane integrity... -
A study on the immunocytochemical localization of eurofascin in rat sciatic nerve
Byung-joon Chang, Ik-hyun Cho and Peter J. Brophy 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 2호 1 67-71 (5 pages)
We examined the localization of neurofascin (NF) in the sciatic nerve of rat. In the myelinated fibers, neurofascin localizes strongly in the nodal axolemma except the small central cleft and also expresses in the paranodes, and weakly in the Schmidt-Lanterman incisures. In the paranodes, NF localizes around the axolemma and it expresses in the apposing membrane of paranodal loops. Axoplasm, compact myelin and cytoplasm of Schwann cell do not express NF at all. In the Schmidt-Lanterman... -
Microbiology,Immunology : Characterization of Yersinia ruckeri isolated from the farm-cultured eel Anguilla japonica in Korea
Seong Joon Joh , Chang Hee Kweon , Min Jeong Kim , Min Su Kang , Hwan Jang , Jun Hun Kwon 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 7 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2010, 제 50권 제 1호 5 29-35 (7 pages)
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Immunocytochemical Study on the Translocation Mechanism of Glucose Transporters by Insulin
Hah, Jong-Sik, Kim, Ku-Ja 대한생리학회 대한생리학회지 16 Pages
대한생리학회 대한생리학회지 1993, 제 27권 제 2호 2 123-138 (16 pages)
The mechanism of insulin action to increase glucose transport is attributed to glucose transporter translocation from intracellular storage pools to the plasma membrane in insulin-sensitive cells. The present study was designed to visualize the redistribution of the glucose transporter by means of an immunogold labelling method. Our data clearly show that glucose transporter molecules were visible by this method. According to the method this distribution of glucose transporters between cell... -
Control of Basal Stem Rot Disease in Oil Palm by Supplementation of Calcium, Copper, and Salicylic Acid
한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 5호 3 396-406 (11 pages)
of Ca/Cu/SA was found to be the most effective in controlling the disease and the high performance liquid chromatography results showed the detection of ergosterol at very low concentration in the treated samples. Moreover, the transmission electron microscopy analysis results clearly indicated that T7 treatment was also enhancing lignification, which was responsible for the thickness of the secondary cell walls and middle lamella compared to untreated samples. It was therefore, concluded that... -
Evaluation of Resistance to Ralstonia solanacearum in Tomato Genetic Resources at Seedling Stage
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 1호 8 58-64 (7 pages)
Bacterial wilt of tomatoes caused by Ralstonia solanacearum is a devastating disease that limits the production of tomato in Korea. The best way to control this disease is using genetically resistant tomato plant. The resistance degree to R. solanacearum was evaluated for 285 tomato accessions conserved in the National Agrobiodiversity Center of Rural Development Administration. These accessions of tomato were originated from 23 countries. Disease severity of tomato accessions was investigated... -
Antimicrobial Effects of a Hexapetide KCM21 against Pseudomonas syringae pv. tomato DC3000 and Clavibacter michiganensis subsp. michiganensis
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2014, 30권 3호 2 245-253 (9 pages)
electron microscopy. The effects of KCM21 on Gram-negative strain, Pseudomonas syringae pv. tomato DC3000 and Gram-positive strain, Clavibacter michiganensis subsp. michiganensis were compared. Treatment with divalent cations such as Ca and Mg inhibited the bactericidal activities of KCM21 significantly against P. syringae pv. tomato DC3000. The bactericidal kinetic study showed that KCM21 killed both bacteria rapidly and the process was faster against C. michiganensis subsp. michiganensis. The... -
The Sensor Kinase GacS Negatively Regulates Flagellar Formation and Motility in a Biocontrol Bacterium, Pseudomonas chlororaphis O6
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2014, 30권 2호 14 215-219 (5 pages)
The GacS/GacA two component system regulates various traits related to the biocontrol potential of plant-associated pseudomonads. The role of the sensor kinase, GacS, differs between strains in regulation of motility. In this study, we determined how a gacS mutation changed cell morphology and motility in Pseudomonas chlororaphis O6. The gacS mutant cells were elongated in stationary-phase compared to the wild type and the complemented gacS mutant, but cells did not differ in length in... -
Open Access : Altered Gene Expression and Intracellular Changes of the Viable But Nonculturable State in Ralstonia solanacearum by Copper Treatment
한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2013, 29권 4호 3 374-385 (12 pages)
completely suppressed bacterial colony formation on culture media; however, LIVE/DEAD BacLight bacterial viability staining showed that the bacterial cells maintained viability, and that the viable cells contain higher level of DNA. Based on electron microscopic observations, the bacterial cells in the VBNC state were unchanged in size, but heavily aggregated and surrounded by an unknown extracellular material. Cellular ribosome contents, however, were less, resulting in a reduction of the total... -
Different Profiles of the Negatively Stained Citrus Canker Bacterium Xanthomonas citri pv. citri Depending on Culture Media and Heavy Metal Stains
한국식물병리학회 The Plant Pathology Journal 3 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 1호 13 90-92 (3 pages)
often showed relatively intact cell morphology and rather positively stained cells with a thin electron-dense stain depth around bacteria. The PTA-stained bacteria were characterized by the wrinkled cell surface where the stain was entrapped in grooves. In addition, distinct electron-dense stain depth was evident around the PTA-stained preparations. Numerous fimbriae could be mostly observed from the PTA-stained preparations of the two culture media, but not from the UA-stained preparations. -
Effects of Motor Learning on Motor Function and Synaptic Plasticity of Cerebellar Injuries
김기도 한국보건기초의학회 한국보건기초의학회지 6 Pages
한국보건기초의학회 한국보건기초의학회지 2020, Vol.13 No.2 8 106-111 (6 pages)
cerebellar cortex was lower in the experimental groups than group I. However group III’s expression of synaptophysin was higher than group II. In electron microscopy findings, the experimental groups showed degeneration of the Purkinje cell. These result suggest that improved motor function by motor learning, after harmaline induced cerebellar injuries, is associated with dynamically altered expression of synaptophysin in the cerebellar cortex and that it is related with synaptic plasticity. -
Effects of synthesis pressure conditions on the preparation of graphene-like carbonaceous materials from organic liquid precursors by a solvothermal method
Kwan-Woo Kim, Byoung Suhk Kim, Sang Won Lee, Dong Chul Chung, Byung-Joo Kim 한국탄소학회 Carbon Letters 5 Pages
한국탄소학회 Carbon Letters 2017, Vol.23 11 74-78 (5 pages)
Carbon materials with at least one dimension of 1?100 nm are called carbon nanomaterials. A significant body of previous research in physics, chemistry and biology has demonstrated that the properties of nano-composites are significantly different than those of the bulk materials [1]. Some unique phenomena, such as the quantum tunneling effect, and surface effects, have also been observed, and attributed to the large surface area and small size of the carbon nanomaterials. It has been reported... -
Synthesis and characterization of carbon doped TiO₂ photocatalysts supported on stainless steel mesh by sol-gel method
JO. Tijani, OO. Fatoba, TC. Totito, WD. Roos, LF. Petrik 한국탄소학회 Carbon Letters 12 Pages
한국탄소학회 Carbon Letters 2017, Vol.22 6 48-59 (12 pages)
several analytical methods: high resolution scanning electron microscopy (HRSEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), and X-ray photoelectron spectroscopy (XPS). The XRD patterns showed that the supported TiO₂ nanocrystals are typically anatase, polycrystalline and body-centered tetragonal in structure. The EDS and XPS results complemented one another and confirmed the presence of carbon species in or on the TiO₂ layer, and the XPS data... -
Synthesize multi-walled carbon nanotubes via catalytic chemical vapour deposition method on Fe-Ni bimetallic catalyst supported on kaolin
Aliyu A, Abdulkareem AS, Kovo AS, Abubakre OK, Tijani JO, Kariim I 한국탄소학회 Carbon Letters 18 Pages
한국탄소학회 Carbon Letters 2017, Vol.21 5 33-50 (18 pages)
catalyst is utilised to produce multi-walled carbon nanotubes (MWCNTs) using catalytic chemical vapour deposition (CCVD) method. The catalysts and MWCNTs prepared using the optimal conditions are characterized using high resolution transmission electron microscope (HRTEM), high-resolution scanning electron microscope (HRSEM), electron diffraction spectrometer (EDS), selected area electron diffraction (SAED), thermogravimetric analysis (TGA), Brunauer-Emmett-Teller (BET), and X-ray diffraction... -
Electrochemical synthesis of nanosized hydroxyapatite/graphene composite powder
Vesna Mi?kovi?-Stankovi?, Sanja Erakovi?, Ana Jankovi?, Maja Vuka?inovi?-Sekuli?, Miodrag Mitri?, Young Chan Jung, Soo Jin Park, Kyong 한국탄소학회 Carbon Letters 8 Pages
한국탄소학회 Carbon Letters 2015, Vol.16 No.4 2 233-240 (8 pages)
of 9.0. The field emission scanning electron microscopy and transmission electron microscopy observations of the composite HAP/Gr powder indicated that nanosized hydroxyapatite particles were uniformly placed in the graphene overlay. Raman spectroscopy, Fourier transform infrared spectroscopy and X-ray diffraction confirmed graphene incorporation in the HAP/Gr powder. The electrochemically prepared HAP/Gr composite powder exhibited slight antibacterial effect against the growth of the bacterial... -
Non-blinking dendritic crystals from C-dot solution
Ashmi Mewada, Ritesh Vishwakarma, Bhushan Patil, Chinmay Phadke 한국탄소학회 Carbon Letters 4 Pages
한국탄소학회 Carbon Letters 2015, Vol.16 No.3 8 211-214 (4 pages)
Bio-imaging and drug carriers for delivery have created a huge demand for crystals. Crystals are fascinating materials that have been grown for a long time but obtaining biocompatible fluorescent crystals is a challenging task. We report on the growth of fluorescent crystals using a carbon dot (C-dot) solution by a hydrothermal process. The crystallization pattern of these C-dots exhibited a unique dendritic structure having a feather-like morphology. The growth temperature and pressure were... -
Water and oxygen permeation through transparent ethylene vinyl alcohol/(graphene oxide) membranes
Hye Min Kim, Heon Sang Lee 한국탄소학회 Carbon Letters 7 Pages
한국탄소학회 Carbon Letters 2014, Vol.15 No.1 7 50-56 (7 pages)
We prepared ethylene vinyl alcohol (EVOH)/graphene oxide (GO) membranes by solution casting method. X-ray diffraction analysis showed that GOs were fully exfoliated in the EVOH/GO membrane. The glass transition temperatures of EVOH were increased by adding GOs into EVOH. The melting temperatures of EVOH/GO composites were decreased by adding GOs into EVOH, indicating that GOs may inhibit the crystallization of EVOH during non-isothermal crystallization. However, the equilibrium melting... -
Carbon nanofiber-reinforced polymeric nanocomposites
Changwoon Jang, John Hutchins, Jaesang Yu 한국탄소학회 Carbon Letters 9 Pages
한국탄소학회 Carbon Letters 2013, Vol.14 No.4 1 197-205 (9 pages)
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Graphene growth from polymers
Hong-Kyu Seo, Tae-Woo Lee 한국탄소학회 Carbon Letters 7 Pages
한국탄소학회 Carbon Letters 2013, Vol.14 No.3 2 145-151 (7 pages)
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솔더볼 조성에 의한 피로강도의 영향
김경수, 김진영 한국진공학회 韓國眞空學會誌 5 Pages
한국진공학회 韓國眞空學會誌 2004, Vol.13 No.3 127-131 (5 pages)
BGA(ball grid array) package에서 솔더볼의 피로강도에 대한 솔더 조성에 대한 영향을 조사하기 위하여 패키지 신뢰성 시험을 실시하였다. 공정조성 솔더 63Sn/37Pb, 62Sn/36Pb/2Ag, 63Sn/34.4Pb/2Ag/0.5Sb 솔더를 이용하여 제조된 시편을 MRT Lv3 (moisture resistance test level) 조건에서 전처리 후 T/C(temperature cycle test) 실험을 수행하였다. 각각의 신뢰성 시험에 대하여 전단강도를 측정하였으며, 미세 조직 사진을 얻었다. 또한, SEM (scanning electron microscope)과 EDX (energy dispersive X-ray)를 이용하여 파괴... -
고속전단 시험을 이용한 Sn-37Pb BGA solder joints의 기계적 신뢰성 특성 평가
장진규, 하상수, 하상옥, 이종근, 문정탁, 박재현, 서원찬, 정승부, Jang. Jin-Kyu, Ha. Sang-Su, Ha. Sang-Ok, Lee. Jong-Gun, Moon. Jung-Tak, Park. Jai-Hyun, Seo. Won-Chan, 한국마이크로전자및패키징학회 마이크로전자 및 패키징 학회지 6 Pages
한국마이크로전자및패키징학회 마이크로전자 및 패키징 학회지 2008, Vol.15 No.4 65-70 (6 pages)
연구에서는 BGA(Ball Grid Array) 솔더 접합부에 high impact가 가해졌을 경우 접합부의 기계적 특성에 대해서 연구하였다. 시편은 ENIG(Electroless Nickel Immersion Gold) 표면 처리된 FR-4 기판 위에 직경이 500 ${mu}m$인 Sn-37Pb 솔더볼을 BGA 방식으로 배열하고 리플로우(Reflow)를 통하여 제작하였다. HTS(High Temperature Storage) 테스트를 위해, 시편을 일정한 온도의 $120^{circ}C$에서 250시간 동안 시효처리(Aging)를 실시하였다. 시효처리 후, 각각의 시편은 고속 전단 시험기(Dage-4000HS)를 이용하여 속도 변수는 0.01,... -
마우스 성숙난자의 유리화 동결법에 따른 동결 융해 후의 염색체와 방추사의 분석
고정재, 박성은, 정형민, 신태은 한국동물번식학회 韓國家畜繁殖學會誌 6 Pages
한국동물번식학회 韓國家畜繁殖學會誌 2001, Vol.25 No.3 287-292 (6 pages)
노출 후 electron microscope grid에 부착시킨 후 액체질소에서 냉동 보존하였다. 동결 융해 후 생존한 난자들은 방추사의 이상성을 보기 위해 immunostaining 방법을, 염색체의 이상성을 보기 위해 karyotyping을 시행하였다. 실험결과 염색체 이상빈도는 대조군의 경우 19.6%, 동결 융해군은 32.8%로 관찰되었고, 방추사의 이상빈도는 대조군의 경우 20.2%, 동결 융해군의 경우 32.3%로 대조군에 비해 염색체와 방추사의 이상성이 증가되었다. 결론적으로 마우스의 성숙난자를 유리화 동결시킨 후 염색체와 방추사의 이상성이 증가됨을...
