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A novel low-cost method for Mycobacterium avium subsp. paratuberculosis DNA extraction from an automated broth culture system for real-time PCR analysis
Miguel Salgado*, Cristobal Verdugo, Cord Heuer, Pedro Castillo, Patricia Zamorano 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 9 233-239 (7 pages)
simple, efficient, and low-cost method of harvesting DNA from Map cultured in liquid medium was developed. The proposed protocol (Universidad Austral de Chile [UACH]) was evaluated by comparing its performance to that of two traditional techniques (a QIAamp DNA Stool Mini Kit and cethyltrimethylammonium bromide [CTAB] method). The results were statistically assessed by agreement analysis for which differences in the number of cycles to positive (CP) were compared by Student’s t-test for paired... -
Cost Behavior During the Global Financial Crisis: The Case of Korean Commercial Banks
Seok-Young Lee 인문사회과학기술융합학회 예술인문사회융합멀티미디어논문지 8 Pages
인문사회과학기술융합학회 예술인문사회융합멀티미디어논문지 2015, 제 5권 제 2호 44 387-394 (8 pages)
In general, for the pooled sample, total operating costs and other operating costs show anti-cost stickiness, but interest costs indicate cost stickiness. Furthermore, the financial crisis of 2007-2008 attenuated the anti-stickiness of total operating costs and other operating costs and reinforced the stickiness of interest costs during this crisis period. However, the worldwide financial crisis of 2008-2009 triggered by the collapse of Bear Stearns and Lehman Brothers continued to attenuate the... -
A Review of Cost-Effectiveness Analysis and Model of Hearing Aid Fitting
Soo Hee Oh, Hyungi Chun, Kyoungwon Lee, Junghak Lee 한국청각언어재활학회 Audiology and Speech Research 6 Pages
한국청각언어재활학회 Audiology and Speech Research 2017, 제 13권 제 2호 1 95-100 (6 pages)
supported a considerable success with incremental cost-effectiveness of hearing aid intervention. A dynamic patient flow model is particularly useful to simulate ongoing decision problems in the use of hearing aids and to estimate cost-effectiveness of hearing aid intervention. Conclusion: The patient flow model proposed in this study involves various possible transitions of people with hearing complaints in South Korea and it applies to the measurement of cost benefits of hearing aid fitting. -
Do increased availability and reduced cost of early childhood care and education narrow social inequality gaps in utilization? Evidence from Norway
Erin Sibley, Eric Dearing, Claudio O Toppelberg, Arnstein Mykletun, Henrik Daae Zachrisson 육아정책연구소 International Journal of Child Care and Education Policy 20 Pages
육아정책연구소 International Journal of Child Care and Education Policy 2015, 제 9권 1 1-20 (20 pages)
other care arrangements. Progressive Norwegian child care policies were also expected to reduce the effect of child and family characteristics that might make a family less likely to enroll their child in center-based care. Using a large sample from the Norwegian Mother and Child Cohort Study (MoBa, n?=?60,270), this research examined selection factors predicting enrollment in center-based ECEC at age 18 months across cohorts from 2002 to 2006. While families with higher parental education and... -
Replacement Model Based on Cost and Downtime
Jung. Ki-Mun, Han. Sung-Sil, Lim. Jae-Hak 한국데이터정보과학회 한국데이터정보과학회지 13 Pages
한국데이터정보과학회 한국데이터정보과학회지 2003, Vol.14 No.4 889-901 (13 pages)
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A comparison of the aerobic cost and muscle use in aerobic dance to the energy costs and muscle use on treadmill, elliptical trainer and bicycle ergometry
Jerrold Petrofsky, M. Laymon, R. Mcgrew, D. Papa, R. Hahn, R. Kaethler, M. Johnson, B. Wernow, D. Poblete 물리치료재활과학회 Physical Therapy Rehabilitation Science 9 Pages
물리치료재활과학회 Physical Therapy Rehabilitation Science 2013, 제 2권 제 1호 3 12-20 (9 pages)
study. Methods: Fifteen female subjects were evaluated to assess the energy cost and muscle activity during a 20 minute dance video compared to treadmill, elliptical track and bicycle ergometry. The later 3 forms of exercise were accomplished in four, 5 minute bouts at different intensities of exercise. Subjects were in the age range of 22-24 years old, were free of cardiovascular disease and did not have any neurological injuries. They were not sedentary and exercised at least twice a week.... -
전통신앙에서 영혼의 천도를 위한 사령제의 목적은 살아있는 사람을 위한 인간 중심주의의 발현이었다. 왜냐하면 영혼이 현세에 계속 머물러 있으면 살아있는 사람에게 해를 주므로 망자는 이승에서의 한을 풀고 어서 저승으로 가기를 권하는 의식이었다. 유교적 전통과 기독교적 죽음관 속에서 그 유사성이 발견된다는 사실은 흥미로운 일이다. 구약성서에서 의인(義人)은 제명을 다하지 못하고 죽더라도 안식을 얻을 수 있다고 하였는데 이는 몸을 죽여서라도 인(仁)을 이루고자 하였던 유교적 선비정신과 일치하고 있다. 전통상례에서...
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Receiver operating characteristic curve analysis of the timed up and go test as a predictive tool for fall risk in persons with stroke
Seung-yeop Lim, Byung-jun Lee, Wan-hee Lee 물리치료재활과학회 Physical Therapy Rehabilitation Science 7 Pages
물리치료재활과학회 Physical Therapy Rehabilitation Science 2018, 제 7권 제 2호 2 54-60 (7 pages)
Objective: Persons with chronic stroke fall more often than healthy elderly individuals. The Timed Up and Go test (TUG) is used as a fall prediction tool, but only provides a result for the total measurement time. This study aimed to determine the optimal cut-off values for each of the 6 components of the TUG. Design: Retrospective study. Methods: Thirty persons with chronic stroke participated in the study. TUG evaluation was performed using a wearable miniaturized inertial sensor. Sensitivity,... -
Comparison of spatio-temporal gait parameters according to shoe types in chronic stroke survivors
Soung Kyun Hong, Su Ho Park, Sung Ri Shin, Dong Geon Lee, Seung Hoo Lee, Sun Hye Jung, Seung Hyeon Pyo, Kyeong-Bong Lee, Gyu Chang Lee 물리치료재활과학회 Physical Therapy Rehabilitation Science 6 Pages
물리치료재활과학회 Physical Therapy Rehabilitation Science 2018, 제 7권 제 1호 5 23-28 (6 pages)
Objective: The purpose of this study was to investigate the impact of wearing various types of shoes on gait ability in stroke survivors and in order to gain information in regards to shoes that could possibly replace ankle orthosis. Design: Cross-sectional study. Methods: Eight hemiplegic survivors diagnosed with stroke participated in the study. Gait was analyzed using the GAITRite Electronic Walkway (CIR System Inc., USA) when subjects walked with no showed, walked with non-ankle-covered... -
Effects of sit-to-stand imagery group training on balance performance in individuals with chronic hemiparetic stroke
Jeong-won Lee, Su-jin Hwang, Sin-ae Ahn 물리치료재활과학회 Physical Therapy Rehabilitation Science 7 Pages
물리치료재활과학회 Physical Therapy Rehabilitation Science 2016, 제 5권 제 2호 2 63-69 (7 pages)
Objective: To evaluate the effects of sit-to-stand (STS) imagery group training (IGT) on STS movement and balance performance for chronic hemiparetic stroke. Design: Randomized controlled trial. Methods: A total of 30 people with chronic hemiparetic stroke (15 for STS-IGT group and 15 for control) were recruited in this study. The STS-IGT group participated in a videotape-based STS-IGT for 30 minutes a day, five days a week for six weeks, while the control group watched a documentary on... -
Comparison of multifidus and external oblique abdominis activity in standing position according to the contraction patterns of the gluteus maximus
Hyuk-Soon Choi, Su-Young Lee 물리치료재활과학회 Physical Therapy Rehabilitation Science 7 Pages
물리치료재활과학회 Physical Therapy Rehabilitation Science 2016, 제 5권 제 1호 7 40-46 (7 pages)
Objective: The purpose of this study was to examine the effect on multifidus and external oblique abdominis muscle activation during hip contraction of three types (concentric, isometric, eccentric) in standing position. Design: Cross-sectional study. Methods: Twenty healthy adult men volunteered to participate in this study. Muscle activation was recorded from gluteus maximus, both multifidus, and both external oblique abdominis by surface electromyography (EMG) while holding position in the... -
Effects of mobile texting and gaming on gait with obstructions under different illumination levels
Jae-yun Cha, Hyun-jin Kim, Jae-myoung Park, Chang-ho Song 물리치료재활과학회 Physical Therapy Rehabilitation Science 6 Pages
물리치료재활과학회 Physical Therapy Rehabilitation Science 2015, 제 4권 제 1호 6 32-37 (6 pages)
Objective: This study was conducted to test the effects of mobile texting and gaming on gait with obstructions under different illumination levels. Design: Cross-sectional study. Methods: Twelve healthy adults aged 20 to 36 years (mean 23.5 years) were tested under six different conditions. All participants used touchscreen smartphones. Testing conditions included: 1) Walking with an obstruction under a bright illumination level; 2) walking with an obstruction with a low level of illumination;... -
Changes of spatio-temporal gait parameters according to experience falls in post-stroke patients
Ki Hun Cho, Wan Hee Lee 물리치료재활과학회 Physical Therapy Rehabilitation Science 6 Pages
물리치료재활과학회 Physical Therapy Rehabilitation Science 2012, 제 1권 제 1호 5 22-27 (6 pages)
Results: The spatial gait parameters and the temporal gait parameters were significantly different between the stroke with falls group and the stroke without falls group (p＜0.05). Furthermore, step length was the only significantly different among symmetry ratio (p＜0.05). Conclusions: Experience of falls can lead to impairment of gait ability in stroke patients. This result is expected to be used as a basic data for rehabilitation program development to prevent a fall of post-stroke patients. -
Prevalence, Antibiotic Resistance, and Virulence Features of Shiga Toxin-producing Escherichia coli Isolates from the Retail Meats and Meat by-products in Korea
June Bong Lee, Dalmuri Han, Jeong Hoon Park, Hyung Tae Lee, Hyun Seok Joh, Yeon Soo Chung, Suk Shin, Yong Ho Park , Chong Hae Hong, Jang 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 81 180-181 (2 pages)
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An enhanced immunochromatographic strip test using colloidal gold nanoparticle-labeled dual-type N proteins for detection of antibodies to PRRS virus
Ji Eun Yu, In-Ohk Ouh, Hyeonjeong Kang, Hye-young Lee, Kwang-Myun Cheong, In-Soo Cho, Sang-Ho Cha 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 4호 7 519-527 (9 pages)
Porcine reproductive and respiratory syndrome (PRRS) is recognized as one of the most important infectious diseases causing serious economic loss in the swine industry worldwide. Due to its increasing genetic diversity, a rapid and accurate diagnosis is critical for PRRS control. The immunochromatographic strip test (ICST) is a rapid and convenient type of immunoassay. In this study, an on-site immunochromatographic assay-based diagnostic method was developed for detection of PRRS virus... -
Molecular cloning and characterization of porcine ribosomal protein L21
Wu-Sheng Sun, Ju-Lan Chun, Dong-Hwan Kim, Jin-Seop Ahn, Min-Kyu Kim, In-Sul Hwang, Dae-Jin Kwon, Seongsoo Hwang, Jeong-Woong Lee 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 4호 18 531-540 (10 pages)
Ribosomal protein L21 (RPL21) is a structural component of the 60S subunit of the eukaryotic ribosome. This protein has an important role in protein synthesis and the occurrence of hereditary diseases. Pig is a common laboratory model, however, to the best of our knowledge, its RPL21 gene has not been cloned to date. In this study, we cloned and identified the full-length sequence of the pig RPL21 gene for the first time. In addition, we examined its expression pattern and function by using... -
Transgenesis for pig models
Soo-Young Yum, Ki-Young Yoon, Choong-Il Lee, Byeong-Chun Lee, Goo Jang 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 3호 1 261-268 (8 pages)
Animal models, particularly pigs, have come to play an important role in translational biomedical research. There have been many pig models with genetically modifications via somatic cell nuclear transfer (SCNT). However, because most transgenic pigs have been produced by random integration to date, the necessity for more exact gene-mutated models using recombinase based conditional gene expression like mice has been raised. Currently, advanced genome-editing technologies enable us to generate... -
Production of α 1,3-galactosyltransferase targeted pigs using transcription activator-like effector nuclease-mediated genome editing technology
Jung-Taek Kang, Dae-Kee Kwon, A-Rum Park, Eun-Jin Lee, Yun-Jin Yun, Dal-Young Ji, Kiho Lee, Kwang-Wook Park 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 11 89-96 (8 pages)
Recent developments in genome editing technology using meganucleases demonstrate an efficient method of producing gene edited pigs. In this study, we examined the effectiveness of the transcription activator-like effector nuclease (TALEN) system in generating specific mutations on the pig genome. Specific TALEN was designed to induce a double-strand break on exon 9 of the porcine α1,3-galactosyltransferase (GGTA1) gene as it is the main cause of hyperacute rejection after xenotransplantation.... -
Retrospective surveillance of metabolic parameters affecting reproductive performance of Japanese Black breeding cows
Urara Watanabe, Mitsuhiro Takagi*, Osamu Yamato, Takeshige Otoi, Koji Okamoto 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 15 283-288 (6 pages)
This retrospective study was conducted to confirm the relationship between pre- and postpartum metabolic parameters and postpartum reproductive performance and to clarify seasonal characteristics of the metabolic parameters by using our metabolic profile test (MPT) database of Japanese Black breeding herds. In evaluation 1, MPT databases of blood samples from multiparous cows collected prepartum and postpartum were divided into two groups according to calving interval, and each MPT parameter was... -
Development and clinical evaluation of a rapid diagnostic kit for feline leukemia virus infection
Won-Shik Kim, Chom-Kyu Chong, Hak-Yong Kim, Gyu-Cheol Lee, Wooseog Jeong, Dong-Jun An, Hye-Young Jeoung, Jae-In Lee, Young-Ki Le 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 10 91-97 (7 pages)
Feline leukemia virus (FeLV) causes a range of neoplastic and degenerative diseases in cats. To obtain a more sensitive and convenient diagnosis of the disease, we prepared monoclonal antibodies specific for the FeLV p27 to develop a rapid diagnostic test with enhanced sensitivity and specificity. Among these antibodies, we identified two clones (hybridomas 8F8B5 and 8G7D1) that specifically bound to FeLV and were very suitable for a diagnostic kit. The affinity constants for 8F8B5 and 8G7D1... -
Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis
Alfredo García, Remigio Martínez, José Manuel Benitez-Medina, David Risco, Waldo Luis García, Joaqu& 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 17 491-494 (4 pages)
Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus(D.)congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used...
