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전체 선택해제
총 488건 검색
본문검색 결과만 보기
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Flow Cytometric Analysis of Nuclear DNA Content, Mitotic Chromosome Number and Protein Separation by SDS-PAGE in Three Accessions of African Locust Bean (Parkia biglobosa Benth.)
Uyoh. Edak Aniedi, Urua. Ikootobong Sunday, Ntui. Valentine Otang, Okpako. Elza Cletus 한국작물학회 Journal of crop science and biotechnology 6 Pages
한국작물학회 Journal of crop science and biotechnology 2011, Vol.14 No.4 227-232 (6 pages)
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Effects of parental folate deficiency on the folate content, global DNA methylation, and expressions of FR${alpha}$, IGF-2 and IGF-1R in the postnatal rat liver
Mejos. Karen Kay, Kim. Hye Won, Lim. Eun Mi, Chang. Namsoo 한국영양학회 Nutrition research and practice 6 Pages
한국영양학회 Nutrition research and practice 2013, Vol.7 No.4 281-286 (6 pages)
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Histopathological and DNA Content Analysis of a Dermal Sarcoma in the Soft-shelled Turtle Pelodiscus sinensis
Syasina. Iraida Germogenovna, Hur. Jun-Wook, Kim. Eun-Mi, Park. In-Seok 한국수산과학회 Journal of fisheries science and technology 8 Pages
한국수산과학회 Journal of fisheries science and technology 2006, Vol.9 No.3 107-114 (8 pages)
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Mitochondrial and DNA damage in bovine somatic cell nuclear transfer embryos
In-Sun Hwang, Hyo-Kyung Bae, Hee-Tae Cheong 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 1 235-240 (6 pages)
reactive oxygen species (ROS) and subsequent mitochondrial and DNA damage in bovine somatic cell nuclear transfer (SCNT) embryos were examined. Bovine enucleated oocytes were electrofused with donor cells and then activated by a combination of Ca-ionophore and 6-dimethylaminopurine culture. The H2O2 and ·OH radical levels, mitochondrial morphology and membrane potential (ΔΨ), and DNA fragmentation of SCNT and in vitro fertilized (IVF) embryos at the zygote stage were analyzed. The H2O2 (35.6... -
Random amplified polymorphic DNA (RAPD) analysis of Mycobacterium tuberculosis strains in India
J. P. N. Singh, Rishendra Verma, P. Chaudhuri 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 15 181-187 (7 pages)
The usefulness of random amplification of polymorphic DNA (RAPD) analysis for typing Indian strains of M. tuberculosis was investigated. M. tuberculosis H37Rv, M. tuberculosis DT and 42 clinical isolates of M. tuberculosis were subjected to RAPD-PCR using 7 random decamer primers. All 7 primers were found to be differentiated and produced specific RAPD profiles. The polymorphic amplicons served as RAPD markers for M. tuberculosis. The dendrograms, obtained by different primers, showed the... -
Flow cytometric evaluation on the age-dependent changes of testicular DNA contents in rats
Chang Yong Yoon, Choong Man Hong, Yong-Yeon Cho, Ji Young Song, I Jin Hong, Dae Hyun Cho, Beom Jun Lee, Hee Jong Song, Cheol Kyu 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 1호 7 43-46 (4 pages)
An age-dependent cellular change of DNA contents in the testis of Sprague-Dawley rats was investigated by flow-cytometric method. Testicular cell suspensions at the age of 4, 5, 6, 7, 8, 10, 12, 16 and 26 weeks were prepared and stained with propidium iodide. The relative proportions in the number of mature and immature haploid (1n), diploid (2n), S-phase and tetraploid (4n) cells were calculated. The proportion in the number of mature haploid cells was sharply increased to the age of 10 weeks... -
성견의 실험적 치아이동시 치주조직내 DNA 합성에 관한 면역조직화학적 연구
김성진(Seong-Jin Kim), 임나원(Na-Won Lim), 김상철(Sang-Cheol Kim) 대한치과교정학회 The Korean Journal of Orthodontics 13 Pages
대한치과교정학회 The Korean Journal of Orthodontics 1996, 제 26권 제 4호 4 359-371 (13 pages)
교정력에 의한 성견의 치아이동시 발생하는 조직변화 중 치주인대 내에서의 세포활성도의 변화를 교정력의 크기와 기간에 따라 알아 보고자 하였다. 생후 1년 6개월된 성견 6마리를 5마리의 실험군과 1마리의 대조군으로 나누었고 실험군에서 하악 좌측에는 강한 힘(250-300g)을, 하악 우측에는 약한 힘(50-75g)을 제1소구치와 제2소구치 사이에 open coil spring으로써 적용하였다. 실험군의 성견을 각각 12시간, 24시간, 3일, 1주, 2주에 Bromodeoxyuridine(BrdU)을 주입하려고 희생시켰다. 하악 제1,2소구치 부위의 조직을 채득하여... -
Long-term Activation of c-Jun N-terminal Kinase through Receptor Interacting Protein is Associated with DNA Damage-induced Cell Death
JeongHoSeok, KyeongAhPark, HeeSunByun, MinhoWon, SangheeShin, Byung-LyulChoi, HyunjiLee, Young-RaeKim, JangHeeHong, JongsunPark, GangMinHu 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2008, Vol.12 No.4 9 185-192 (8 pages)
a member of the mitogen-activated protein kinase family, is an important cellular response that modulates the outcome of the cells which are exposed to the tumor necrosis factor (TNF) or the genotoxic stress including DNA damaging agents. Although it is known that JNK is activated in response to genotoxic stress, neither the pathways to transduce signals to activate JNK nor the primary sensors of the cells that trigger the stress response have been identified. Here, we report that the receptor... -
Reduction of TNFα-induced Oxidative DNA Damage Product, 8-Hydroxy-2′-Deoxyguanosine, in L929 Cells Stably Transfected with Small Heat Shock Protein
Young-MeePark, Eun-MiChoi 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 11 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1997, Vol.1 No.2 11 209-219 (11 pages)
which has been increasingly recognized as one of the most sensitive markers of oxidative DNA damage. Our results clearly demonstrated that the level of 8 ohdG increased in L929 cells in a TNFα dose-dependent manner. Subsequently, we asked whether small HSP has a protective effect on TNFα-induced oxidative DNA damage. To accomplish this goal, we have stably transfected L929 cells with mouse small hsp cDNA (hsp25) since these cells are devoid of endogenous small hsps. We found that TNFα-induced... -
Microbial Community Dysbiosis and Functional Gene Content Changes in Apple Flowers due to Fire Blight
Hyun Gi Kong, Hyeonheui Ham, Mi-Hyun Lee, Dong Suk Park, Yong Hwan Lee 한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 4호 10 404-412 (9 pages)
Despite the plant microbiota plays an important role in plant health, little is known about the potential interactions of the flower microbiota with pathogens. In this study, we investigated the microbial community of apple blossoms when infected with Erwinia amylovora. The long-read sequencing technology, which significantly increased the genome sequence resolution, thus enabling the characterization of fire blight- induced changes in the flower microbial community. Each sample showed a unique... -
Taxonomic Position of Korean Isolates of Rhizoctonia solani Based on RAPD and ITS Sequencing of Ribosomal DNA
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 1호 12 83-89 (7 pages)
by randomly amplified polymorphic DNA (RAPD) and sequence analyses of the internal transcribed spacer (ITS) regions of ribosomal DNA. All the isolates within each group showed highly similar band patterns in RAPD. The ITS regions of the isolates within the same groups showed a high level of sequence similarity above 96.0% whereas similarities among different groups were below 94.4%. When compared with several reference strains of R. solani from foreign countries, all the Korean isolates were... -
연초에서 발생하는 복숭아혹진딧물(Myzus persicae)형태형 2종의 Random Amplified Polymorphic DNA(RAPD)을 이용한 유전적 유연관계 분석
채순용, 이기원, 김상석, 장영덕 한국응용곤충학회 한국응용곤충학회지 7 Pages
한국응용곤충학회 한국응용곤충학회지 1998, Vol.37 No.1 31-37 (7 pages)
GC content가 70, 80, 90%인 primer에서 각각 26.9%, 50.5% 및 66.^%로 GC content가 높아질수록 PCR결과 band의 양상이 좋게 나타났다. simple matching coefficient를 구하여 matrix를 작성해 본 결과 유사계수(similarity coefficient)의 범위는 0.414~0.808사이이었다. 복숭아혹진딧물 clone 간에 유사계수가 가장 높은 것은 PG2와 PG3 클론으로 0.808로 나타났으며, DBR클론을 기준으로 하여 볼 때 PG2 클론간의 유사계수를 0.414로 유사도가 가장 낮았다. 유사계수를 이용하여 8가지 클론의 진딧물들에 대한 유전적 근연관RP를... -
Antioxidant Activity and Protection from DNA Damage by Water Extract from Pine (Pinus densiflora) Bark
Jiang. Yunyao, Han. Woong, Shen. Ting, Wang. Myeong-Hyeon 한국식품영양과학회 Preventive nutrition and food science 6 Pages
한국식품영양과학회 Preventive nutrition and food science 2012, Vol.17 No.2 116-121 (6 pages)
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초위성체 DNA를 이용한 제주마 집단의 품종특성 및 개체식별 체계설정
조병욱, 정지혜, 김상욱, 김희수, 이학교, 조길재, 송기덕, Cho. Byung-Wook, Jung. Ji-Hye, Kim. Sang-Wook, Kim. Heui-Soo, Lee. Hak-Kyo, Cho. Gil-Jae, Song. Ki-Duk 한국생명과학회 생명과학회지 6 Pages
한국생명과학회 생명과학회지 2007, Vol.17 No.10 1441-1446 (6 pages)
본 연구는 서로 다른 상염색체에 위치하고 있는 초위성체유전 표지를 활용하여 제주마 집단의 타 품종과의 차별적 유전특성분석과 제주마 집단에서 활용할 수 있는 효율적인 개체 식별 시스템 설정을 위해 수행되었다. 공시재료로서는 5품종에서 총 191두가 사용되었으며 13종의 좌위에 대한 개체별 유전자형을 분석하였다. 이들 13종에서 출현된 총 대립 유전자수는 제주마의 경우 155종이 나타났다. 한국 제주마 집단에서 나타난 평균 이형접합도는 0.317-0.902였으며 marker 다형성 정보량은 0.498-0.799로 나타났다. 제주마 집단에서... -
Hanwoo individual identification with DNA marker information
Lee. Jea-Young, Choi. Yu-Mi 한국데이터정보과학회 한국데이터정보과학회지 10 Pages
한국데이터정보과학회 한국데이터정보과학회지 2007, Vol.18 No.3 599-608 (10 pages)
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방사선에 의한 염색체이상과 DNA 함량과의 관계
정해원, 김수영, 하성환, 김태환, 조철구, Chung. Hai-Won, Kim. Su-Young, Ha. Sung-Whan, Kim. Tae-Hwan, Cho. Chul-Koo 대한방사선방어학회 방사선방어학회지 11 Pages
대한방사선방어학회 방사선방어학회지 2001, Vol.26 No.2 101-111 (11 pages)
DNA 양을 감안한 기대치와 관찰치의 차이를 비교함으로서 각 염색체의 방사선에 대한 감수성을 평가하여 궁극적으로 방사선 피폭시 생물학적 선량계로서 FISH기법의 타당성을 평가하고자 하였다. 1번 및 4번 염색체의 경우 상호전좌와 이동원 염색체의 관찰치가 기대치보다 더 높게 나타났으며 이와 반대로 2, 7, 8 및 9번 염색체의 경우 상호전좌와 이동원염색체의 관찰치 모두 기대치보다 낮게 나타났다. 2번 및 4번 염색체의 경우 1번 염색체보다 더 많은 acentric fragment의 빈도를 나타내었다. 1, 2, 및 4번 염색체 3종을 조합했을... -
The use of laser Doppler blood flow to assess the effect of acute administration of vitamin D on micro vascular endothelial function in people with diabetes
Jerrold Petrofsky, Faris Alshammari, Iman Akef Khowailed, Riya Lodha, Pooja Deshpande, Praveen Rajaram, Mahendra Gaikwad, Vidhi Vadera 물리치료재활과학회 Physical Therapy Rehabilitation Science 7 Pages
물리치료재활과학회 Physical Therapy Rehabilitation Science 2013, 제 2권 제 2호 1 63-69 (7 pages)
Objective: To assess the effect of vitamin D administration on the skin blood flow response to occlusion and heat. Design: Cross-sectional study. Methods: Twenty age matched subjects; 10 who had diabetes and 10 who were controls were administered 4,000 IU of vitamin D3 for 3 weeks at breakfast. The function of the endothelial cells was evaluated in 2 ways; first, the response to 4 minutes of vascular occlusion of the skin was measured with a laser Doppler flow meter. Second, the skin blood flow... -
Prevention and Intervention of Adolescents’ Maladaptive Internet use
Oh Yeon Ju 경인교육대학교 교육연구원 The Journal of Education 15 Pages
경인교육대학교 교육연구원 The Journal of Education 2019, Vol.2 No.2 1 1-15 (15 pages)
This article presents the characteristics of addiction, cognitive behavior, and social cognitive perspectives, which are the mainstream views of maladaptive Internet use. As the academic branch dealing with mental health, such as psychology and psychiatry, first raised the issue of maladaptive Internet use and continued research, views in the context of mental health, such as addiction and cognitive behavior perspectives, have become mainstream. The mental health perspective, however, shows... -
The efficacy of gaseous chlorine dioxide as a fumigant at livestock farm
Yang ho Jang, Kwang jick Lee, Mi An, Jae young Song 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 122 396-396 (1 pages)
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Detection of Lawsonia intracellularis in Horses using Serology and PCR in the Republic of Korea
Mukter Hossain, Sung Hyun Moon, Byoung Joo Seo, Won Il Kim, Byeong Yeal Jung, Se Ji Cho, Eu Tteum Song, Jae Won Byun, Ho Seong Cho 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 89 184-185 (2 pages)
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Transforming growth factor-beta induced epithelial- mesenchymal transition abolished without TGF-beta condition: Cancer stem cell properties and cisplatin resistance in lungcancer cell lines
Seong Kwan Kim, Jin A Park, Sang Hyun Cho, Weijia Zheing, Jeong Min Choi, Ho Chul Shin 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 232 336-337 (2 pages)
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Promoting Stem Cell Proliferation by Vegetable Resources
Mi Jeong Park, Da Un Jeong, Na Yeon Gu, Jeong Su Byeon, In Soo Cho, Sang Ho Cha, Jienny Lee 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 230 335-336 (2 pages)
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Porcine intestinal lymphoid tissues synthesize estradiol
Chan Jin Park, Heehyen Kim, Jooyoung Jin, Radwa Barakat, Po-Ching Lin, Jeong Moon Choi, CheMyong Jay Ko 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 4호 1 477-482 (6 pages)
Estradiol (17-estradiol) is synthesized primarily in the gonads of both sexes and regulates the development and function of reproductive organs. Recently, we reported that intestinal lymphocyte homeostasis is regulated by estradiol synthesized de novo in the endothelial cells of the high endothelial venules (HEVs) of mesenteric lymph nodes and Peyer’s patches in mice. This observation prompted us to hypothesize that HEVs of intestinal lymphoid tissues are sites of estradiol synthesis... -
Rapid visual detection of Mycobacterium avium subsp. paratuberculosis by recombinase polymerase amplification combined with a lateral flow dipstick
Guimin Zhao, Hongmei Wang, Peili Hou, Chengqiang He, Hongbin He 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 10 242-250 (9 pages)
testing is infrequent due to the lack of a robust method. The isothermal recombinase polymerase amplification (RPA) technique has applied for rapid diagnosis. Herein, RPA combined with a lateral flow dipstick (LFD) assay was developed to estimate DNA from Mycobacterium avium subsp. paratuberculosis. First, analytical specificity and sensitivity of the RPA-nfo primer and probe sets were assessed. The assay successfully detected M. paratuberculosis DNA in 30 min at 39°C with a detection limit of... -
Analysis of structure-function relationship in porcine rotavirus A enterotoxin gene
Sharad Saurabh, Shubhankar Sircar, Jobin J. Kattoor, Souvik Ghosh, Nobumichi Kobayashi, Krisztian Banyai, Obli R. VinodhKumar, U 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 6 35-43 (9 pages)
Rotavirus (RV)-infected piglets are presumed to be latent sources of heterologous RV infection in humans and other animals. In RVs, non-structural protein 4 (NSP4) is the major virulence factor with pleiotropic properties. In this study, we analyzed the nsp4 gene from porcine RVs isolated from diarrheic and non-diarrheic cases at different levels of protein folding to explore correlations to diarrhea-inducing capabilities and evolution of nsp4 in the porcine population. Full-length nsp4 genes... -
Fluoroscopic characteristics of tracheal collapse and cervical lung herniation in dogs: 222 cases (2012?2015)
Jeosoon Lee, Sookyung Yun, Inhyung Lee, Mincheol Choi, Junghee Yoon 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 4호 15 499-505 (7 pages)
This retrospective study included 222 dogs suspected to have tracheal collapse (TC) and examined between 1 January 2012 and 31 December 2015. Fluoroscopic and radiographic images and medical records were evaluated to investigate the fluoroscopic characteristics of TC and cervical lung herniation (CLH), and to determine their relationships to various influencing. Grade of TC, existence of tracheal kinking, location of CLH (right or left side), and grade of CLH were evaluated fluoroscopically. One... -
Infections by pathogens with different transmission modes in feral cats from urban and rural areas of Korea
Jusun Hwang, Nicole Gottdenker, Dae-Hyun Oh, Hang Lee, Myung-Sun Chun 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 4호 14 541-545 (5 pages)
In this study, we examine prevalences of three infectious pathogens with different transmission modes (Bartonella henselae, hemoplasma, and Toxoplasma gondii) in feral cats from urban and rural habitats. Infection status of the three pathogens in blood samples (n = 117) was determined through molecular or serological diagnostic methods. Overall prevalence of hemoplasma, Toxoplasma gondii, and Bartonella henselae was 47.9%, 50%, and 35.7%, respectively. Comparing the two habitats, only... -
Effect of phosphorus deficiency on erythrocytic morphology and function in cows
Ziwei Zhang, Mingyu Bi, Jie Yang, Haidong Yao, Zhonghua Liu, Shiwen Xu 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 10 333-340 (8 pages)
cows. Activities of superoxide dismutase and glutathione peroxidase in erythrocytes were lower, while the malondialdehyde content was greater, in P-deficient cows than in control diet-fed cows. Na/K-ATPase and Mg-ATPase activities were lower in P-deficient cows than in control diet-fed cows; however, Ca-ATPase activity was not significantly different. The phospholipid composition of the erythrocyte membrane changed and membrane fluidity rigidified in P-deficient cows. The results indicate that P... -
Alterations in antioxidant function and cell apoptosis in duck spleen exposed to molybdenum and/or cadmium
Mengmeng Zhang, Junrong Luo, Caiying Zhang, Huabin Cao, Bing Xia, Guoliang Hu 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 9 193-200 (8 pages)
To investigate the effects of molybdenum (Mo) and/or cadmium (Cd) on antioxidant function and the apoptosis-related genes in duck spleens. Sixty healthy 11-day-old ducks were randomly divided into six groups of 10 ducks (control, low Mo group, high Mo, Cd, low Mo + Cd, and high Mo + Cd groups). All were fed a basal diet containing low or high dietary doses of Mo and/or Cd. Relative spleen weight, antioxidant indices, apoptosis-related gene mRNA expression levels, and ultrastructural changes were...
