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전체 선택해제
총 787건 검색
본문검색 결과만 보기
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Pathology Parasitology : Production of Theileria sergenti recombinant protein by E coli expression system
ParkJinHo, ChaeJoonSeok, KimDaeHyuk, JangYongSuk, KwonOhDeog, LeeJooMook 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 11 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 1999, 제 39권 제 4호 17 786-796 (11 pages)
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Characterization of Carboxymethylcellulase(CMCase) Produced by Recombinant E. coli Containing CMCase Gene for Cellulomonas sp. YE-5
Park. Sung-Won, Her. Nam-Yun, Kim. Dong-Seob, Park. Sun-Jin, Lee. Han-Seung, Park. Hak-Jong, Yu. Ju-Hyun 한국식품영양과학회 Journal of food science and nutrition 6 Pages
한국식품영양과학회 Journal of food science and nutrition 1997, Vol.2 No.2 174-179 (6 pages)
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Comparison of Statistical Methods for Optimization of Salts in Medium for Production of Carboxymethylcellulase of Bacillus amyloliquefaciens DL-3 by a Recombinant E. coli JM109/DL-3
이유정, 김혜진, 고와, 정정한, 이진우, Lee. You-Jung, Kim. Hye-Jin, Gao. Wa, Chung. Chung-Han, Lee. Jin-Woo 한국생명과학회 생명과학회지 9 Pages
한국생명과학회 생명과학회지 2011, Vol.21 No.9 1205-1213 (9 pages)
재조합 균주인 E. coli JM109/DL-3를 사용하여 carboxymethylcellulase를 생산하기 위한 배지의 최적 염 농도를 orthogonal array method (OAM)과 response surface method (RSM) 등과 같은 통계학적인 방법으로 확립하고 그 결과를 비교하였다. OAM에 기초를 한 Qualitek-4 Software를 사용하여 실험을 계획하고, 그 결과를 분석한 결과는 K2HPO4가 균체의 생장 및 carboxymethylcellulase의 생산에 미치는 영향이 가장 크다는 사실을 확인하였다. 균체의 생육에 최적인 $K_2HPO_4$, NaCl, $MgSO_4{cdot}7H_2O$ 및 $(NH_4)_2SO_4$의... -
Expression of Recombinant Hybrid Peptide Gaegurin4 and LL37 using Fusion Protein in E. coli
바야르바트 이쉬반질, 이재학, 이순열, Bayarbat. Ishvaanjil, Lee. Jae-Hag, Lee. Soon-Youl 한국미생물생명공학회 한국미생물·생명공학회지 6 Pages
한국미생물생명공학회 한국미생물·생명공학회지 2012, Vol.40 No.2 92-97 (6 pages)
항균 펩타이드(Antimicrobial peptides(AMPs)는 그람 양성, 그람 음성 세균과 진균병원체에 대항하는 생명체에서 중요한 역할을 하는 물질이다. 인간의 Cathelicidin 항균 펩타이드는 임상학적으로 사용할 수 있는 여러 가지의 생물학적 활성을 가진다. 항균 펩타이드의 생산 비용은 재조합 방법으로 낮출 수가 있다. 대장균은 저렴하며 손쉬운 조작이 가능하기 때문에 다른 재조합 단백질처럼 항균 펩타이드의 발현에 훌륭한 숙주가 될 수 있다. 그러나 대장균에서의 항균 펩타이드의 과발현은 항균 펩타이드가 과발현 되었을 때... -
Comparative Study on Characterization of Recombinant B Subunit of E. coli Heat-Labile Enterotoxin (rLTB) Prepared from E. coli and P. pastoris
Ma. Xingyuan, Yao. Bi, Zheng. Wenyun, Li. Linfeng 한국미생물생명공학회 Journal of microbiology and biotechnology 8 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2010, Vol.20 No.3 550-557 (8 pages)
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Extracellular Overproduction of $eta$-Cyclodextrin Glucanotransferase in a Recombinant E. coli Using Secretive Expression System
Lee. Kwang-Woo, Shin. Hyun-Dong, Lee. Yong-Hyun 한국미생물생명공학회 Journal of microbiology and biotechnology 7 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2002, Vol.12 No.5 753-759 (7 pages)
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Expression of the Functional Recombinant Interleukin-16 in E. coli and Mammalian Cell Lines
Kim. Seon-Young, Lee. Chang-Hun, Kim. Kyung-Joo, Kim. Yeon-Soo 한국미생물생명공학회 Journal of microbiology and biotechnology 8 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2001, Vol.11 No.2 234-241 (8 pages)
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The Basis of Different Sensitivities of Ovarian Cancer Cells to the Recombinant Adenoviral Vector System Containing a Tumor-Specific L-plastin Promoter and E. coli Cytosine Deaminase Gene as a Transcription Unit
Chung. In-Jae 한국응용약물학회 Biomolecules & therapeutics 6 Pages
한국응용약물학회 Biomolecules & therapeutics 2009, Vol.17 No.2 138-143 (6 pages)
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High Cell Density Fed-batch Fermentation for the Production of Recombinant E. coli K-12 Ghost Vaccine against Streptococcal Disease
Park. Seung-Jun, Lee. Seok-Joo, Kim. Ki-Hong, Kim. Sung-Koo 한국생물공학회 Biotechnology and bioprocess engineering 6 Pages
한국생물공학회 Biotechnology and bioprocess engineering 2011, Vol.16 No.4 733-738 (6 pages)
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Optimization of Medium Components for Plasmid Production by Recombinant E. coli $DH5{alpha}$ $pUK21CMV{eta}1.2$
Zheng. Shuzhen, Friehs. Karl, He. Ning, Deng. Xu, Li. Qingbiao, He. Zheng, Xu. Chenyun, Lu. Yinghua 한국생물공학회 Biotechnology and bioprocess engineering 9 Pages
한국생물공학회 Biotechnology and bioprocess engineering 2007, Vol.12 No.3 213-221 (9 pages)
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N-Terminal pI Determines the Solubility of a Recombinant Protein Lacking an Internal Transmembrane-like Domain in E. coli
Lee. Sang-Jun, Han. Yun-Hee, Kim. Young-Ok, Nam. Bo-Hye, Kong. Hee-Jeong, Kim. Kyung-Kil 한국분자세포생물학회 Molecules and cells 9 Pages
한국분자세포생물학회 Molecules and cells 2010, Vol.30 No.2 127-135 (9 pages)
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Novel Modification of Growth Medium Enables Efficient E. coli Expression and Simple Purification of an Endotoxin-Free Recombinant Murine Hsp70 Protein
Zachova. Katerinat, Krupka. Michal, Chamrad. Ivo, Belakova. Jana, Horynova. Milada, Weigl. Evzen, Sebela. Marek, Raska. Milan 한국미생물생명공학회 Journal of microbiology and biotechnology 7 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2009, Vol.19 No.7 727-733 (7 pages)
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Production of Coenzyme $Q_{10}$ by Recombinant E. coli Harboring the Decaprenyl Diphosphate Synthase Gene from Sinorhizobium meliloti
Seo. Myung-Ji, Im. Eun-Mi, Hur. Jin-Haeng, Nam. Jung-Yeon, Hyun. Chang-Gu, Pyun. Yu-Ryang, Kim. Soon-Ok 한국미생물생명공학회 Journal of microbiology and biotechnology 6 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2006, Vol.16 No.6 933-938 (6 pages)
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In Vitro Formation of Protein Nanoparticle Using Recombinant Human Ferritin H and L Chains Produced from E. coli
RO. HYEON SU, PARK. HYUN KYU, KIM. MIN GON, CHUNG. BONG HYUN 한국미생물생명공학회 Journal of microbiology and biotechnology 5 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2005, Vol.15 No.2 254-258 (5 pages)
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Process Strategies to Enhance the Production of 5-Aminolevulinic Acid with Recombinant E. coli
LEE. DAE HEE, JUN. WOO JIN, YOON. JEONG WEON, CHO. HONG YON, HONG. BUM SIHK 한국미생물생명공학회 Journal of microbiology and biotechnology 8 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2004, Vol.14 No.6 1310-1317 (8 pages)
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Production of Poly(3-hydroxybutyrate) [P(3HB)] with High P(3HB) Content by Recombinant Escherichia coli Harboring the Alcaligenes latus P(3HB) Biosynthesis Genes and the E. coli ftsZ Gene
Choi. Jong-Il, Lee. Sang-Yup 한국미생물생명공학회 Journal of microbiology and biotechnology 4 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 1999, Vol.9 No.6 722-725 (4 pages)
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Purification and Characterisation of a Burkholderia pseudomallei Protease Expressed in Recombinant E. coli
Ling. Jessmi M.L., Nathan. Sheila, Hin. Lee Kok, Mohamed. Rahmah 생화학분자생물학회 Journal of biochemistry and molecular biology 8 Pages
생화학분자생물학회 Journal of biochemistry and molecular biology 2001, Vol.34 No.6 509-516 (8 pages)
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Construction and characterization of intergenotypic chimeric avian hepatitis E viruses (HEVs) with the genotype 2 avian HEV hypervariable region in the backbone of genotype 1 avian HEV
Hyun Woo Moon, Haan Woo Sung, Hyuk Moo Kwon 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 108 245-246 (2 pages)
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Immunogenicityand protective efficacy of recombinant Stx2e toxoids in mice
Dong-ju Shin, Nak-hyung Lee 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 269 355-356 (2 pages)
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A Salmonella Typhi ghost induced by the E gene of phage X174 stimulates dendritic cells and efficiently activates the adaptive immune response
Gayeon Won, Seong Kug Eo, Sang-Youel Park, Jin Hur, John Hwa Lee 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 4호 9 536-542 (7 pages)
Previously, we genetically engineered a Salmonella Typhi bacterial ghost (STG) as a novel inactivated vaccine candidate against typhoid fever. The underlying mechanism employed by the ghost in stimulating the adaptive immune response remains to be investigated. In this study, we aimed to evaluate the immunostimulatory effect of STG on mouse bone marrow-derived dendritic cells (BMDCs) and its activation of the adaptive immune response in vitro. Immature BMDCs were stimulated with STG, which... -
Recombinant-attenuated Salmonella Pullorum strain expressing the hemagglutinin-neuraminidase protein of Newcastle disease virus (NDV) protects chickens against NDV and Salmonella Pullorum challenge
Ke Ding, Ke Shang, Zu-Hua Yu, Chuan Yu, Yan-Yan Jia, Lei He, Cheng-Shui Liao, Jing Li, Chun-Jie Zhang, Yin-Ju Li, Ting-Cai Wu, X 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 9 232-241 (10 pages)
the chickens immunized with the LaSota live vaccine (p ＞ 0.05). Moreover, the novel strain provides 60% and 80% protective efficacy against the NDV virulent strain F48E9 and the S. Pullorum virulent strain C79-13. In summary, in this study, a recombinant-attenuated S. Pullorum strain secreting NDV HN protein was constructed. The generation of the S. Pullorum C79-13ΔcrpΔasd (pYA-HN) strain provides a foundation for the development of an effective living-vector double vaccine against ND and PD. -
Immunogenicity of recombinant Lactobacillus plantarum NC8 expressing goose parvovirus VP2 gene in BALB/c mice
Yu-Ying Liu, Wen-Tao Yang, Shao-Hua Shi, Ya-Jie Li, Liang Zhao, Chun-Wei Shi, Fang-Yu Zhou, Yan-Long Jiang, Jing-Tao Hu, Wei Gu, 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 5 159-167 (9 pages)
a vector to express the VP2 gene of GPV, and recombinant L. plantarum pSIP409-VP2/NC8 was successfully constructed. The molecular weight of the expressed recombinant protein was approximately 70 kDa. Mice were immunized with a 2 × 10 colony-forming unit/200 μL dose of the recombinant L. plantarum strain, and the ratios and numbers of CD11c, CD3CD4, CD3CD8, and interferon gamma- and tumor necrosis factor alpha-expressing spleen lymphocytes in the pSIP409-VP2/NC8 group were higher than those in... -
Improvement of pregnancy rate by intrauterine administration of dexamethasone and recombinant human leukemia inhibitory factor at the time of embryo transfer in cattle
Sangho Roh, Se-Woong Kim, Yeon-Gil Jung, Jong-Im Park 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 17 569-576 (8 pages)
Dex; 1,000 U/mL recombinant human leukemia inhibitory factor (rhLIF); or Dex+rhLIF. Although the rates to development to the blastocyst were not significantly different among groups, the hatching rate after additional culture with Dex +/or rhLIF was significantly higher in all supplemented groups than the control (p ＜ 0.05). In the presence of PGF2α, the hatching rate was significantly restored in all supplemented groups relative to the group treated with only PGF2α and the control... -
Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein
Alisha Wehdnesday Bernardo Reyes, Hannah Leah Tadeja Simborio, Huynh Tan Hop, Lauren Togonon Arayan, Suk Kim 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 16 119-122 (4 pages)
The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but not reactive in the middle or late stage, and was reactive to Brucella-positive mouse serum in the late stage, but not in the early or middle stage of infection. In addition, rMDH did not react with Brucella-negative bovine or mouse sera. These results suggest that rMDH has the potential for use... -
Hepatitis E virus as an emerging zoonotic pathogen
Woo-Jung Park, Byung-Joo Park, Hee-Seop Ahn, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, Han-Sang Yoo, In-Soo 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 1 1-11 (11 pages)
Hepatitis E outbreaks are a serious public health concern in developing countries. The disease causes acute infections, primarily in young adults. The mortality rate is approximately 2%; however, it can exceed 20% in pregnant women in some regions in India. The causative agent, hepatitis E virus (HEV), has been isolated from several animal species, including pigs. HEV genotypes 3 and 4 have been isolated from both humans and animals, and are recognized as zoonotic pathogens. Seroprevalence... -
Characterisation of genotype VII Newcastle disease virus (NDV) isolated from NDV vaccinated chickens, and the efficacy of LaSota and recombinant genotype VII vaccines against challenge with velogenic NDV
Kiarash Roohani, Sheau Wei Tan, Swee Keong Yeap, Aini Ideris, Mohd Hair Bejo, Abdul Rahman Omar 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 8 447-457 (11 pages)
and HN genes showed that both velogenic viruses used in this study, genotype VII NDV isolate IBS002 and genotype VIII NDV isolate AF2240-I, had high genetic variations with genotype II LaSota vaccine. In this study, the protection efficacy of the recombinant genotype VII NDV inactivated vaccine was also evaluated when added to an existing commercial vaccination program against challenge with velogenic NDV IBS002 and NDV AF2240-I in commercial broilers. The results indicated that both LaSota and... -
Enhancing mucosal immunity in mice by recombinant adenovirus expressing major epitopes of porcine circovirus-2 capsid protein delivered with cytosine-phosphate-guanosine oligodeoxynucleotides
Hong-tao Chang, Xiu-yuan He, Yu-Feng Liu, Lu Chen*, Quan-hai Guo, Qiu-ying Yu, Jun Zhao, Xin-wei Wang, Xia Yang, Chuan-qing Wang 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 8 399-407 (9 pages)
A recombinant replication-defective adenovirus expressing the major epitopes of porcine circovirus-2 (PCV-2) capsid protein (rAd/Cap/518) was previously constructed and shown to induce mucosal immunity in mice following intranasal delivery. In the present study, immune responses induced by intranasal immunization with a combination of rAd/Cap/518 and cytosine-phosphate-guanosine oligodeoxynucleotides (CpG ODN) were evaluated in mice. The levels of PCV-2-specific IgG in serum and IgA in saliva,...
