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Antagonistic Activities of Bacillus spp. Strains Isolated from Tidal Flat Sediment Towards Anthracnose Pathogens Colletotrichum acutatum and C. gloeosporioides in South Korea
한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 2호 8 165-175 (11 pages)
gloeosporioides. The three Bacillus atrophaeus strains, LB14, HM03, and HM17, produced large quantities of chitinase and protease enzymes, whereas the B. amyloliquefaciens strain LB01 produced protease and cellulase enzymes. Two important antagonistic traits, siderophore production and solubilization of insoluble phosphate, were observed in the three B. atrophaeus strains. Analyses of disease suppression revealed that LB14 was most effective for suppressing the incidence of anthracnose symptoms... -
Bacillus spp. 엽면살포에 의한 가로수 및 고추의 병 방제
정준휘, 류충민 한국식물병리학회 식물병연구 13 Pages
한국식물병리학회 식물병연구 2016, 22권 2호 3 81-93 (13 pages)
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Cellulolytic Enzymes Production from Submerged Fermentation of Different Substrates by Newly Isolated Bacillus spp. FME
Kumar. G. Satheesh, Chandra. M. Subhosh, Sumanth. M., Vishnupriya. A., Reddy. B. Rajasekhar, Choi. Yong-Lark 한국응용생명화학회 한국응용생명화학회지 5 Pages
한국응용생명화학회 한국응용생명화학회지 2009, Vol.52 No.1 17-21 (5 pages)
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Effect of Rice Bran and Soybean Fermented by Bacillus spp. on Lipid Profiles of Liver, Serum, and Feces in Rats Fed High Fat Diet
Lim. Seong-Il, Seong. Ki-Seung, Song. Sun-Mi, Hwang. Jin-Taek, Lee. Boo-Yong 한국응용생명화학회 Journal of the Korean Society for Applied Biological Chemistry 6 Pages
한국응용생명화학회 Journal of the Korean Society for Applied Biological Chemistry 2011, Vol.54 No.2 237-242 (6 pages)
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인삼 점무늬병균(Alternaria panax)과 탄저병균(Colletotrichum gloeosporioides)에 대한 길항미생물 Bacillus spp. 선발
이혜진, 박기춘, 이승호, 방경환, 박홍우, 현동윤, 강승원, 차선우, 정일민, Lee. Hye-Jin, Park. Kee-Choon, Lee. Seung-Ho, Bang. Kyong-Hwan, Park. Hong-Woo, Hyun. Dong-Yun, Ka 한국약용작물학회 韓國藥用作物學會誌 6 Pages
한국약용작물학회 韓國藥用作物學會誌 2012, Vol.20 No.5 339-344 (6 pages)
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혈전용해활성이 우수한 Bacillus spp. 스타터를 이용한 청국장의 발효특성
황종현, Hwang. Jong-Hyun 한국식품영양과학회 한국식품영양과학회지 7 Pages
한국식품영양과학회 한국식품영양과학회지 2010, Vol.39 No.12 1832-1838 (7 pages)
점질물 형성 효과를 갖는 7개의 Bacillus 균주를 선정하였다. 다음으로 청국장의 기능성을 증진시키기 위해 우수한 혈전용해활성을 갖는 균주를 starter로 이용하기 위해 fibrin plate법으로 활성을 검토한 결과, CJJN-4와 5가 tripticase soy broth에서 가장 높은 활성을 나타냄을 확인할 수 있었다. 한편, 혈전용해 능이 우수한 CJJN-4와 CJJN-5를 이용하여 $40^{circ}C$와 $45^{circ}C$에서 청국장을 발효한 후 활성을 측정한 결과에서도 2개의 균주 모두 우수한 활성을 확인할 수 있었다. 또한, 발효시간과 관계없이 $40^{circ}C$에서... -
청국장에서 분리한 Bacillus spp. 균주의 혈전용해능 및 면역증강활성
장진희, 심윤영, 김승호, 지규만, 차성관, Chang. Jin-Hee, Shim. Youn-Young, Kim. Seung-Ho, Chee. Kyoo-Man, Cha. Seong-Kwan 한국식품과학회 한국식품과학회지 6 Pages
한국식품과학회 한국식품과학회지 2005, Vol.37 No.2 255-260 (6 pages)
cytokine 생성능력 (NO 생성능, Interlukin-1${alpha},;TNF-{alpha}$) 검정에 의한 면역세포 활성능 측정실험을 수행하였는데, Bacillus 균주들을 TSB(Trypticase soy broth)에 키운 시료와 콩에 접종하여 제조한 청국장시료와 같은 두 가지 상이한 시료를 이용하여 혈전용해능과 면역세포 활성능을 측정한 결과, 혈전용해능과 면역세포 활성능이 가장 우수한 세 균주가 최종 선발되었다. 선발된 세균주의 동정결과 두 균주(CHKJ 1249, CHKJ 1326)는 Bacillus licheniformis로 그리고 CHKJ 1339 균주는 Bacillus subtilis로 동정되었다. -
Characterization of Two Urease-Producing and Calcifying Bacillus spp. Isolated from Cement
Achal. Varenyam, Mukherjee. Abhijit, Reddy. M. Sudhakara 한국미생물생명공학회 Journal of microbiology and biotechnology 6 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2010, Vol.20 No.11 1571-1576 (6 pages)
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Molecular Mechanism of Plant Growth Promotion and Induced Systemic Resistance to Tobacco Mosaic Virus by Bacillus spp.
Wang. Shuai, Wu. Huijun, Qiao. Junqing, Ma. Lingli, Liu. Jun, Xia. Yanfei, Gao. Xuewen 한국미생물생명공학회 Journal of microbiology and biotechnology 9 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2009, Vol.19 No.10 1250-1258 (9 pages)
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Molecular Differentiation of Bacillus spp. Antagonistic Against Phytopathogenic Fungi Causing Damping-off Disease
Cho. Min-Jeong, Kim. Young-Kwon, Ka. Jong-Ok 한국미생물생명공학회 Journal of microbiology and biotechnology 8 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2004, Vol.14 No.3 599-606 (8 pages)
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Bacillus spp.를 이용한 옥수수 밑둥썩음병의 생물학적 방제
한준희, 박기창, 김준오, 김경수 한국식물병리학회 식물병연구 10 Pages
한국식물병리학회 식물병연구 2015, 21권 4호 5 280-289 (10 pages)
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볏짚에서 분리한 청국장 starter용 Bacillus spp.의 생리적 특성
이신호, 백낙민, 박나영, Lee. Shin-Ho, Baek. Lag-Min, Park. La-Young 한국식품과학회 한국식품과학회지 6 Pages
한국식품과학회 한국식품과학회지 2008, Vol.40 No.5 562-567 (6 pages)
API 50CHB kit를 이용하여 동정한 결과 99.0%의 유사율로 Bacillus licheniformis로 간이 동정되었다. 분리균주인 B-59는 pH 2.5로 조정된 인공 위액에 초기 생균수 8.33 CFU/mL에서 $37^{circ}C$에서 3시간 배양 후의 6.91 CFU/mL로 높은 생존율을 나타내었으며, 인공담즙산에서 초기 균수 6.90 CFU/mL에서, 배양 24시간 후 8.24 CFU/mL로 증가하여 내성을 나타내었다. 분리균주인 B-59는 NaCl 0, 2, 4% 농도에서는 각각 7.63, 7.70, 7.67 CFU/mL로 성장에 영향을 미치지 않았으나, 32% 농도에서도 생존하여 NaCl에 대한 내성을... -
Bacillus spp.의 원형질체 형성 및 재생
최기춘, 김광현, 전우복 한국초지조사료학회 한국초지학회지 8 Pages
한국초지조사료학회 한국초지학회지 1997, Vol.17 No.1 11-18 (8 pages)
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Bacillus spp.를 이용한 Biosurfactant 생산공정
허성호, 양지석, 홍정화, Hur. Sung-Ho, Yang. Ji-Seok, Hong. Jeong-Hwa 한국식품영양과학회 한국식품영양과학회지 5 Pages
한국식품영양과학회 한국식품영양과학회지 2002, Vol.31 No.3 389-393 (5 pages)
청국장에서 분리한 Bacillus subtilis CH-1, Bacillus circul문 K-1과 Bacillus subtilis(natto) N-1 모두 biosurfactant를 생성하며 이 중 Bacillus subtilis CH-1가 가장 큰 생성력을 나타냈다. Biosurfactant를 대량 생산하기 위하여 AM, LM, NB과 TSB 배지중 AM을 기본배지로 선정하여 최적 탄소원과 질소원으로 glucose 2%, soy peptone 0.3%와 무기염을 포함하는 합성배지를 완성하였다. Biosurfactant의 생성은 96시간에 최대를 나타냈으며 이때 배지의 표면장력은 초기값의 약 43% 값을 나타냈다. 한편 배양온도 및 pH는... -
갯지렁이(Perinereis aibuhitensis)에서 분리한 Bacillus spp.의 생리생화학적 특성 분석
신세연, 이상석, 강경호, 강형일, Shin. Seyeon, Lee. Sang-Suk, Kang. Kyoung-Ho, Kahng. Hyung-Yeel 한국생명과학회 생명과학회지 11 Pages
한국생명과학회 생명과학회지 2013, Vol.23 No.3 415-425 (11 pages)
종류의 Bacillus 균주 CBW3, CBW4, CBW9, CBW14 그리고 EBW10를 선별하여 그 특성을 비교 분석하였다. 16S rRNA 염기서열에 기초하여 동정한 결과, CBW3과 CBW14는 B. nanhaiensis, B. arsenicus 그리고 B. barbaricus와 99.8% 이상의 높은 상동성을, CBW4, CBW9 그리고 EBW10 균주는 B. anthracis, B. algicoa 그리고 B. thuringiensis와 각각 92.7%, 99.8% 그리고 99.8%의 상동성을 보였다. 이들 대부분 균주들의 생장온도 범위는 $4-45^{circ}C$, 염도는 0-17%, pH는 5-9 범위로 매우 다양하게 나타났다. 모든 균주들이 casein, starch... -
Bacillus sp.유래 $eta$-Mannanase 정제 및 Guar Gum가수분해 올리고당의 Bifidobacterium spp.에 대한 증식활성
최준영, 박귀근 한국미생물생명공학회 한국미생물·생명공학회지 6 Pages
한국미생물생명공학회 한국미생물·생명공학회지 2004, Vol.32 No.2 117-122 (6 pages)
DEAE-sephadex ion exchange column chromatography에 의해 Bacillus sp. 유래 $eta$-mannanase 의정제를 수행하여 비활성 21.57 units/$mell$, 정제배율 95.33을 나타내었다. SDS-PAGE에 의한 단일밴드를 확인하였고, 분자량은 38.9 kDa으로 결정되었다. 정제효소에 의해 guar gum galactomannan을 가수분해하여 1차 activated carbon column chromatography 와 2차 Sephadex G-25 gel filtration에 의해 당가수분해물을 분리 회수하여 TLC 및 FACE 에 의해 주요 당가수분해물은 중합도 5와 7로 확인되었다. B. longum B. bifidum, B.... -
Biocontrol of Citrus Canker Disease Caused by Xanthomonas citri subsp. citri Using an Endophytic Bacillus thuringiensis
Nurul Islam, Sarafat Ali, Seong-Jin Choi, Jae-Wook Hyun, Kwang-Hyun Baek 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 5호 9 486-497 (12 pages)
bacteria were isolated from various gymnospermic and angiospermic plants. They were screened for their antagonistic activities against three wild-type and six streptomycin-resistant Xcc strains. TbL-22 and TbL-26, both later identified as Bacillus thuringiensis, inhibited all the wild and resistant Xcc strains. TbL-22 exerted the highest antagonistic activity against XccW3 and XccM6 with inhibition zones of 20.64 ± 0.69 and 19.91 ± 0.87 mm, respectively. Similarly ethyl acetate extract of... -
Effect of Bacillus aryabhattai H26-2 and B. siamensis H30-3 on Growth Promotion and Alleviation of Heat and Drought Stresses in Chinese Cabbage
Da Jeong Shin, Sung-Je Yoo, Jeum Kyu Hong, Hang-Yeon Weon, Jaekyeong Song, Mee Kyung Sang 한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 2호 10 178-187 (10 pages)
stresses in Chinese cabbages, however, the effects were different to cultivars of Chinese cabbages. In addition to bacterial activity to abiotic stresses, H30-3 could suppress incidence (%) of soft rot in ‘Buram-3-ho’. The tested strains were identified as Bacillus aryabhattai H26-2 and B. siamensis H30-3 based on 16S rRNA gene sequence analysis. Taken together, H26-2 and H30-3 could be candidates for both plant growth promotion and mitigation of heat and drought stresses in Chinese cabbage. -
Enhanced Tolerance of Chinese Cabbage Seedlings Mediated by Bacillus aryabhattai H26-2 and B. siamensis H30-3 against High Temperature Stress and Fungal Infections
Young Hee Lee, Su Jeong Jang, Joon-Hee Han, Jin Su Bae, Hyun-suk Shin, Hee Jin Park, Mee Kyung Sang, Song Hee Han, Kyoung Su Kim, Sang-W 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 10 555-566 (12 pages)
Two rhizobacteria Bacillus aryabhattai H26-2 and B. siamensis H30-3 were evaluated whether they are involved in stress tolerance against drought and high temperature as well as fungal infections in Chinese cabbage plants. Chinese cabbage seedlings cv. Ryeokgwang (spring cultivar) has shown better growth compared to cv. Buram-3-ho (autumn cultivar) under high temperature conditions in a greenhouse, whilst there was no difference in drought stress tolerance of the two cultivars. In vitro growth of... -
Activation of Pathogenesis-related Genes by the Rhizobacterium, Bacillus sp. JS, Which Induces Systemic Resistance in Tobacco Plants
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 2호 13 195-201 (7 pages)
Plant growth promoting rhizobacteria (PGPR) are known to confer disease resistance to plants. Bacillus sp. JS demonstrated antifungal activities against five fungal pathogens in in vitro assays. To verify whether the volatiles of Bacillus sp. JS confer disease resistance, tobacco leaves pre-treated with the volatiles were damaged by the fungal pathogen, Rhizoctonia solani and oomycete Phytophthora nicotianae. Pre-treated tobacco leaves had smaller lesion than the control plant leaves. In... -
Bacillus oryzicola sp. nov., an Endophytic Bacterium Isolated from the Roots of Rice with Antimicrobial, Plant Growth Promoting, and Systemic Resistance Inducing Activities in Rice
한국식물병리학회 The Plant Pathology Journal 13 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 2호 7 152-164 (13 pages)
to the closely related strains, Bacillus siamensis KACC 15859 (99.67%), Bacillus methylotrophicus KACC 13105 (99.65%), Bacillus amyloliquefaciens subsp. plantarum KACC 17177 (99.60%), and Bacillus tequilensis KACC 15944 (99.45%). The DNA-DNA relatedness value between strain YC7010 and the most closely related strain, B. siamensis KACC 15859 was 50.4±3.5%, but it was 91.5±11.0% between two strains YC7007 and YC7010, indicating the same species. The major fatty acids of two strains were... -
근권세균 Bacillus sp. CT16과 Neobacillus sp. JC05의 배양액 추출물에 의한 뿌리혹 선충의 알 부화 억제 효과
장화진, 김상태, 상미경 한국식물병리학회 식물병연구 5 Pages
한국식물병리학회 식물병연구 2021, 27권 2호 2 61-65 (5 pages)
균주는 각각 Bacillus sp. CT16과 Neobacillus sp. JC05로 동정하였으며, 두 균 주의 배양액을 용매로 추출 및 농축한 후 선충의 알 부화율을 평가한 결과, JC05의 n-butanol 추출물은 25 μg/ml이상의 농도 에서 알의 부화를 효과적으로 억제했으며, CT16의 n-hexane과 n-butanol 추출물에서는 각각 25 μg/ml, 10 μg/ml 이상의 농도 에서 알 부화를 효과적으로 억제하였다. 오이 유묘 식물에 검정 한 결과, 100 μg/ml의 CT16와 JC05의 n-butanol 추출물을 오 이 유묘에 처리했을 때, 뿌리에 감염된 난낭의 수가 감소하였다. 이를 통해... -
Bacillus sp.가 세포외로 생산하는 Inulinase의 정제 및 특성
김경남, 최용진 한국미생물생명공학회 산업미생물학회지 6 Pages
한국미생물생명공학회 산업미생물학회지 1990, Vol.18 No.5 490-495 (6 pages)
토양 분리균인 Bacillus spp.가 생산하는 inulinase를 ammonium sulfate 분획, 열처리, DEAE Sephadex Cl-6B ion exchange chromatography, Sephadex G-100 및 Sephadex G-150 gel 여과 등의 과정을 거쳐 단일 단백질로 분리 정제하였다. 정제 inulinase는 분자량이 약 56,000인 효소로서 pH6.0,$50^{circ}C$에서 최대 활성을 나타내었으며 $Co^{2+}$와 $Mn^{2+}$에 의해서 현저한 활성화 효과를 보였다. 또한 본 효소는 sucrose와 raffinose에 대해서도 높은 활성을 나타내므로 $eta$-D-fructofuranosidase(EC 3.2.1.26)로 분류되는... -
Development of a sandwich ELISA for the detection of Listeria spp. using specific flagella antibodies
Seong-Hee Kim, Min-Keun Park, Jin-Young Kim, Pham Duc Chuong, Yong-Soon Lee, Byoung-Su Yoon, Kyu-Kye Hwang, Yoon- Kyu Lim 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 6 41-46 (6 pages)
pair of MAbs (MAbs 2B1 and HRP labeled MAbs 7A3) detected up to 104cells/0.1 ml at 22℃ and 30℃. Detection limit of sandwich ELISA using IgY was 10 times lower than MAb pair. Using the developed ELISA, we could detect several Listeria contaminated in food samples after 48 h-culturing. In conclusion, both MAbs and IgY have been proved to be highly specific to detect Listeria flagella and the developed sandwich ELISA using these antibodies would be useful tool for screening Listeria spp. in food. -
Serovars distribution and antimicrobial resistance patterns of Salmonella spp. isolated from the swine farms and slaughter houses
Ho Kyoung Jung , Sung Seok Lee , Chi Young Kim , Sun Young Sunwoo , Young S. Lyoo 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 6 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2011, 제 51권 제 2호 6 123-128 (6 pages)
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Methylome Analysis of Two Xanthomonas spp. Using Single-Molecule Real-Time Sequencing
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 6호 2 500-507 (8 pages)
of Helicobacter pylori, Lactobacillus spp., and Escherichia coli have been determined, and previously unreported DNA methylation motifs have been identified. However, the methylomes of Xanthomonas species, which belong to the most important plant pathogenic bacterial genus, have not been documented. Here, we report the methylomes of Xanthomonas axonopodis pv. glycines (Xag) strain 8ra and X. campestris pv. vesicatoria (Xcv) strain 85-10. We identified N-methyladenine (6mA) and N-methylcytosine... -
무당벌레(Harmonia axyridis ) 장내세균의 특성 및 Staphylococcus spp. 장내세균이 무당벌레의 발육에 미치는 영향
문청원, 김기광, 황경숙, 서미자, 윤영남, 유용만, Moon. Chung-Woun, Kim. Ki-Kwang, Whang. Kyung-Sook, Seo. Mi-Ja, Youn. Young-Nam, Yu. Yong-Man 한국응용곤충학회 한국응용곤충학회지 9 Pages
한국응용곤충학회 한국응용곤충학회지 2011, Vol.50 No.2 157-165 (9 pages)
70%가 Bacillus속과 Staphylococcus속을 포함하는 계통군이었으며, 시험 무당벌레 소화기관으로부터 공통적으로 분리되는 특징을 보였다. 분리된 장내세균 중 대표세균 18균주를 대상으로 항생제에 대한 감수성 조사를 수행한 결과, ofloxacin과 penicillin이 장내세균의 모두에게 증식을 저해하는 항생제 내성을 나타내어 시험약제로 선택하였다. 무당벌레의 먹이인 복숭아혹진딧물(Myzus persicae)과 무테두리진딧물(Lipaphis eryimi)에 ofloxacin 과 penicillin을 직접 처리하여 무당벌레를 사육하면서 번데기무게, 유충기간, 성충의... -
Lactobacillus spp. 이용 발효 붉나무의 기능성물질 검색에 대한 연구
이동성, 강민수, 김윤철, 임남경, 김현수, 정길생, Lee. Dong-Sung, Kang. Min-Su, Kim. Youn-Chul, Im. Nam-Kyung, Kim. Hyun-Su, Jeong. Gil-Saeng 한국생명과학회 생명과학회지 11 Pages
한국생명과학회 생명과학회지 2013, Vol.23 No.1 44-54 (11 pages)
Lb. brevis KCTC 3498 균주로 각각 발효한 한약재는 발효 전보다 항산화 활성이 높아지거나 비슷한 수준으로 나타났다. Lb. plantarum subsp. plantarum KCTC 3108, Lb. casei KCTC 3109로 발효한 붉나무 발효액과 ethyl acetate 여액층은 Bacillus subtilis PCI 219, Escherichia coli KCTC 1682, Shigella flexneri KCTC 2517, Vibrio parahaemolyticus KCTC 7471, Pseudomonas aeruginosa KCTC 2004에 모두 항균활성을 보였다. 붉나무를 Lb. brevis KCTC 3498로 발효하여 ethyl acetate로 추출한 추출물은 B. subtilis PCI 219, E. col... -
Konjac Glucomannan 가수분해 올리고당의 중합도별 Bifidobacterium spp.에 대한 대사활성
최준영, 박귀근 한국식품영양과학회 한국식품영양과학회지 6 Pages
한국식품영양과학회 한국식품영양과학회지 2004, Vol.33 No.7 1186-1191 (6 pages)
exchange column chromatography에 의해 Bacillus sp. 유래 h-mannanase의 정제를 수행하여 비활성은 21.57 units/mg, 정제배율은 93.78배를 나타내었다. 최적 온도는 5$0^{circ}C$, 최적 pH는 6.0이며, 온도 안정성에서는 30∼5$0^{circ}C$에서는 90%이상의 잔존활성을 나타내었고,70∼8$0^{circ}C$에서는 30%이하의 잔존활성을 나타내었다. pH 안정성에서는 pH 5.5∼7.0에서 100%의 잔존활성을 나타낸 반면 pH 2.0∼4.0에서는 40%이하로 감소되 었다. 정제효소에 의해 konjac glucomannan을 가수분해하여 1차 activated carbon column... -
Identification, Characterization, and Efficacy Evaluation of Bacillus velezensis for Shot-Hole Disease Biocontrol in Flowering Cherry
Viet-Cuong Han, Nan Hee Yu, Hyeokjun Yoon, Neung-Ho Ahn, Youn Kyoung Son, Byoung-Hee Lee, Jin-Cheol Kim 한국식물병리학회 The Plant Pathology Journal 16 Pages
한국식물병리학회 The Plant Pathology Journal 2022, 38권 2호 5 115-130 (16 pages)
we investigated the inhibitory activities of antagonistic bacteria against SH pathogens both in vitro and in vivo as well as their biochemical characteristics and bioactive compounds. Two biosurfactant- producing bacterial antagonists, identified as Bacillus velezensis strains JCK-1618 and JCK-1696, exhibited the best effects against the growth of both bacterial and fungal SH pathogens in vitro through their cell-free culture filtrates (CFCFs). These two strains also strongly inhibited the growt... -
Pathogenicity of Bacillus Strains to Cotton Seedlings and Their Effects on Some Biochemical Components of the Infected Seedlings
Aly A. Aly, Omima M. El-Mahdy, Marian M. Habeb, Abeer Elhakem, Amal A. Asran, Maryan M. Youssef, Heba I. Mohamed, Rania S. Hanafy 한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2022, 38권 2호 3 91-101 (11 pages)
of the infected seedlings were also assayed. The biochemical components included total soluble sugars, total soluble proteins, total free amino acids, peroxidase, polyphenol oxidase, phenols, and lipid peroxidation. ANOVA showed that Bacillus strain (B) was a very highly significant source of variation in damping-off and dry weight. Cotton cultivar (V) was a nonsignificant source of variation in damping-off while it was a significant source of variation in dry weight. B × V interaction was a... -
Effect of Bacillus mesonae H20-5 Treatment on Rhizospheric Bacterial Community of Tomato Plants under Salinity Stress
Shin Ae Lee, Hyeon Su Kim, Mee Kyung Sang, Jaekyeong Song, Hang-Yeon Weon 한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 6호 15 662-671 (10 pages)
effects on microbial succession in the rhizosphere are poorly understood. In this study, the inoculants of Bacillus mesonae strain H20-5 were administered to tomato plants grown in soils with different salinity levels (EC of 2, 4, and 6 dS/m). The bacterial communities in the bulk and rhizosphere soils were examined 14 days after H20-5 treatment using Illumina MiSeq sequencing of the bacterial 16S rRNA gene. Although the abundance of H20-5 rapidly decreased in the bulk and rhizosphere soils, a... -
Control Efficacy of Bacillus velezensis AFB2-2 against Potato Late Blight Caused by Phytophthora infestans in Organic Potato Cultivation
Min Jeong Kim, Chang Ki Shim, Jong-Ho Park 한국식물병리학회 The Plant Pathology Journal 16 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 6호 8 580-595 (16 pages)
of AFB2-2, the disease incidence and severity of potato late blight at 107 cfu/ml was lowest at 0.07 and 6.7, respectively. The nucleotide sequences of AFB2-2 strain were searched in the NCBI GenBank; Bacillus siamensis strain KCTC 13613, Bacillus velezensis strain CR-502, and Bacillus amylo- liquefaciens strain DSM7 were found to have a genetic similarity of 99.7%, 99.7%, and 99.5%, respectively. The AFB2-2 strain was found to harbor the biosynthet- ic genes for bacillomycin D, iturin, and... -
Antagonistic and Plant Growth-Promoting Effects of Bacillus velezensis BS1 Isolated from Rhizosphere Soil in a Pepper Field
Jong-Hwan Shin, Byung-Seoung Park, Hee-Yeong Kim, Kwang-Ho Lee, Kyoung Su Kim 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 3호 10 307-314 (8 pages)
mycelial growth, appressorium formation, and disease development of C. scovillei. By combined sequence analysis using 16S rRNA and partial gyrA sequences, strain BS1 was identified as Bacillus velezen- sis, a member of the B. subtilis species complex. BS1 produced hydrolytic enzymes (cellulase and protease) and iron-chelating siderophores. It also promoted chili pepper (cv. Nockwang) seedling growth compared with untreated plants. The study concluded that B. velezensis BS1 has good potential as... -
Current Classification of the Bacillus pumilus Group Species, the Rubber- Pathogenic Bacteria Causing Trunk Bulges Disease in Malaysia as Assessed by MLSA and Multi rep-PCR Approaches
Ainur Ainiah Azman Husni, Siti Izera Ismail, Noraini Md. Jaafar, Dzarifah Zulperi 한국식물병리학회 The Plant Pathology Journal 15 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 3호 4 243-257 (15 pages)
Bacillus pumilus is the causal agent of trunk bulges dis- ease affecting rubber and rubberwood quality and yield production. In this study, B. pumilus and other closely related species were included in B. pumilus group, as they shared over 99.5% similarity from 16S rRNA analysis. Multilocus sequence analysis (MLSA) of five housekeeping genes and repetitive elements-based poly- merase chain reaction (rep-PCR) using REP, ERIC, and BOX primers conducted to analyze the diversity and systematic... -
Overcoming Encouragement of Dragon Fruit Plant (Hylocereus undatus) against Stem Brown Spot Disease Caused by Neoscytalidium dimidiatum Using Bacillus subtilis Combined with Sodium Bicarbonate
Sanan Ratanaprom, Korakot Nakkanong, Charassri Nualsri, Palakrit Jiwanit, Thanyakorn Rongsawat, Natthakorn Woraathakorn 한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 3호 1 205-214 (10 pages)
The use of the supernatant from a Bacillus subtilis culture mixed with sodium bicarbonate was explored as a means of controlling stem brown spot disease in dragon fruit plants. In in vitro experiments, the B. subtilis supernatant used with sodium bicarbonate showed a strong inhibition effect on the growth of the fungus, Neoscytalidium dimidiatum, the agent causing stem brown spot disease and was notably effective in preventing fungal invasion of dragon fruit plant. This combination not only... -
Chemical Fungicides and Bacillus siamensis H30-3 against Fungal and Oomycete Pathogens Causing Soil-Borne Strawberry Diseases
Bo Reen Park, Hyun Jin Son, Jong Hyeob Park, Eun Soo Kim, Seong Jin Heo, Hae Ree Youn, Young Mo Koo, A Yeong Heo, Hyong Woo Choi, Mee Kyung Sang, Sang-Woo Lee, Sung Hwan Choi, Jeum Kyu Hong 한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 1호 7 79-85 (7 pages)
or L. theobromae, respectively. Bacillus siamensis H30-3 was antagonistic against the three pathogens by diffusible as well as volatile molecules, and evidently reduced aerial mycelial formation of P. cactorum. B. siamensis H30-3 growth was declined by at least 0.025 mg/ml of pyraclostrobin. The two fungicides additively inhibited mycelial growths of C. fructicola, but not of P. cactorum and L. theobromae. B. siamensis H30-3 vola- tiles led to less growth of C. fructicola than one reduced by the... -
Screening and Application of Bacillus Strains Isolated from Nonrhizospheric Rice Soil for the Biocontrol of Rice Blast
Yuexia Sha, Qingchao Zeng, Shuting Sui 한국식물병리학회 The Plant Pathology Journal 13 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 3호 4 231-243 (13 pages)
analyses, S170 and S9 were identified as Bacillus amyloliquefaciens and B. pumilus, respectively. The research also demonstrated that B. amyloliquefaciens S170 and B. pumilus S9 could colonize rice plants to prevent pathogenic infection and evidently suppressed plant disease caused by 11 other plant pathogenic fungi. This is the first study to demonstrate that B. amyloliquefaciens and B. pumilus isolated from nonrhizospheric rice soil are capable of recolonizing internal rice stem tissues. -
Inhibitory Abilities of Bacillus Isolates and Their Culture Filtrates against the Gray Mold Caused by Botrytis cinerea on Postharvest Fruit
Xiaomeng Chen, Yajie Wang, Yu Gao, Tongguo Gao, Dongdong Zhang 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 5호 4 425-436 (12 pages)
has been reported to infect more than 200 crop species worldwide, and it causes massive losses in yield. The aim of this study was to evaluate the inhibitory abilities and effects of Bacillus amyloliquefaciens RS-25, Bacillus licheniformis MG-4, Bacillus subtilis Z-14, and Bacillus subtilis Pnf-4 and their culture filtrates and extracts against the gray mold caused by B. cinerea on postharvest tomato, strawberry, and grapefruit. The results revealed that the cells of Z-14, culture filtrate of... -
Characterization of Bacillus amyloliquefaciens DA12 Showing Potent Antifungal Activity against Mycotoxigenic Fusarium Species
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 5호 7 499-507 (9 pages)
isolated Bacillus amyloliquefaciens strain DA12 from soil and explored its antimicrobial activities. DA12 was active against the growth of mycotoxigenic F. asiaticum, F. graminearum, F. proliferatum, and F. verticillioides both in vitro and in planta (maize). Further screening using dual culture extended the activity range of strain DA12 against other fungal pathogens including Botrytis cinerea, Colletotrichum coccodes, Endothia parasitica, Fusarium oxysporum, Raffaelea quercus-mongolicae, and... -
Diffusible and Volatile Antifungal Compounds Produced by an Antagonistic Bacillus velezensis G341 against Various Phytopathogenic Fungi
한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 5호 6 488-498 (11 pages)
by Bacillus sp. G341 with strong antifungal activity against various phytopathogenic fungi. Strain G341 isolated from four-year-old roots of Korean ginseng with rot symptoms was identified as Bacillus velezensis based on 16S rDNA and gyrA sequences. Strain G341 inhibited mycelial growth of all phytopathogenic fungi tested. In vivo experiment results revealed that n-butanol extract of fermentation broth effectively controlled the development of rice sheath blight, tomato gray mold, tomato late... -
Cyclic Dipeptides from Bacillus vallismortis BS07 Require Key Components of Plant Immunity to Induce Disease Resistance in Arabidopsis against Pseudomonas Infection
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 4호 6 402-409 (8 pages)
promoting rhizobacteria (PGPRs) also produce CDPs that can induce disease resistance. Bacillus vallismortis strain BS07 producing various CDPs has been evaluated as a potential biocontrol agent against multiple plant pathogens in chili pepper. However, plant signal pathway triggered by CDPs has not been fully elucidated yet. Here we introduce four CDPs, cyclo(Gly-L-Pro) previously identified from Aspergillus sp., and cyclo(L-Ala-L-Ile), cyclo(L-Ala-L-Leu), and cyclo(LLeu-L-Pro) identified from... -
Biological Control Potential of Bacillus amyloliquefaciens KB3 Isolated from the Feces of Allomyrina dichotoma Larvae
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 3호 12 273-280 (8 pages)
Bacillus amyloliquefaciens KB3. The inhibitory activity of this strain correlated with lipopeptide productions, including iturin A and surfactin. Production of these surfactants in the KB3 isolate varied with the culture phase and growth medium used. In planta biocontrol activities of cell-free culture filtrates of KB3 were similar to those of the commercial biocontrol agent, B. subtilis QST-713. These results support the presence of microbes with the potential to inhibit fungal growth, such as...
