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Detection of canine distemper virus (CDV) through one step RT-PCR combined with nested PCR
Yong-Hwan Kim, Kyu-Woan Cho, Hwa-Young Youn, Han Sang Yoo, Hong-Ryul Han 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 1호 10 59-63 (5 pages)
dogs. Of the 51 blood samples from dogs clinically suspected of CD, 45 samples were revealed as positive by one-step RT-PCR combined with nested PCR. However, only 15 samples were identified as positive with a single one step RT-PCR. Therefore approximately 60% increase in the efficiency of the diagnosis was observed by the combined method. These results suggested that one step RT-PCR combined with nested PCR could be a sensitive, specific, and practical method for diagnosis of CDV infection. -
Effects of forward & backward walking training with progressive body weight supported on stroke patients’ ambulatory ability
Kyung-Hoon Kim, Suk-Min Lee 물리치료재활과학회 Physical Therapy Rehabilitation Science 9 Pages
물리치료재활과학회 Physical Therapy Rehabilitation Science 2014, 제 3권 제 2호 1 77-85 (9 pages)
of the step length, total double support, cadence, gait were measured using optogait and the 10-m walk test (10MWT), 6 minutes walk test (6MWT). Results: In the within group comparisons, all the three groups showed significant differences between before and after the intervention (p<0.05). In the comparison of the three groups, there were significant differences among the three groups in stride length, double limb support stance, cadence, 10MWT, and 6MWT in the third week, and only in stride... -
Embodied Culture in Pottery: Developing Teacher’s Package for Understanding Other’s Culture and Own Culture
Kim Haikyung 경인교육대학교 교육연구원 The Journal of Education 23 Pages
경인교육대학교 교육연구원 The Journal of Education 2018, Vol.1 No.2 3 29-51 (23 pages)
There are various perspectives on the roles and functions of museums. In modern times, socio-educational functions of museums are the most emphasized. Most museums offer programs for schools, but teachers still prefer not to visit them. The reason why teachers feel pressured to visit a museum is that they lack knowledge about museum exhibits. Also, it takes too much time and energy for individual teachers to reconstruct the curriculum in conjunction with museum exhibits. The link with the school... -
Dembo polymerase chain reaction technique for detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs
Sayed Samim Rahpaya, Shinobu Tsuchiaka, Mai Kishimoto, Mami Oba, Yukie Katayama, Yuka Nunomura, Saki Kokawa, Takashi Kimura, Atsushi Kob 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 4 350-357 (8 pages)
Bovine abortion, diarrhea, and respiratory disease complexes, caused by infectious agents, result in high and significant economic losses for the cattle industry. These pathogens are likely transmitted by various vectors and reservoirs including insects, birds, and rodents. However, experimental data supporting this possibility are scarce. We collected 117 samples and screened them for 44 bovine abortive, diarrheal, and respiratory disease complex pathogens by using Dembo polymerase chain... -
Effects of induced endometritis on uterine blood flow in cows as evaluated by transrectal Doppler sonography
Letizia Debertolis, Gaetano Mari, Barbara Merlo, Sabine Merbach, Heinz-Adolf Schoon, Eleonora Iacono, Heinrich Bollwein 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 8 189-197 (9 pages)
This study was conducted to evaluate the effects of induced endometritis on uterine blood flow in cows. Transrectal Doppler sonography was performed on uterine arteries of six cyclic cows before and for 4 days after inducing acute endometritis by intrauterine infusion of 720 mg of policresulen, and for 4 days of the following estrous cycle. Time-averaged maximum velocity (TAMV) increased (p < 0.001) and pulsatility index (PI) decreased (p < 0.0001) within 1 h of policresulen... -
Quantitative real-time polymerase chain reaction for detecting Mycoplasma hyosynoviae and Mycoplasma hyorhinis in pen-based oral, tonsillar, and nasal fluids
João Carlos Gomes Neto, Leslie Bower, Barbara Z. Erickson, Chong Wang, Matthew Raymond, Erin L. Strait 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 9 195-201 (7 pages)
Mycoplasma (M.) hyorhinis and M. hyosynoviae are pathogens known to cause disease in pigs post-weaning. Due to their fastidious nature, there is increased need for culture-independent diagnostic platforms to detect these microorganisms. Therefore, this study was performed to develop and optimize quantitative real-time PCR (qPCR) assays to rapidly detect M. hyorhinis and M. hyosynoviae in pen-based oral fluids as well as nasal and tonsillar fluids as proxies for samples used in swine herd... -
Prostaglandin F receptor expression in intrauterine tissues of pregnant rats
Elvan Anadol*, Halit Kanca, Atiye Seda Yar, Fatma Helvacioğlu, Sevda Menevşe, Engin Ҫalgüne 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 14 125-131 (7 pages)
In this investigation, we studied the expression and localization of rat prostaglandin F (FP) receptor in uterine tissues of rats on gestational Days 10, 15, 18, 20, 21, 21.5 and postpartal Days 1 and 3 using Western blotting analysis, real-time PCR, and immunohistochemistry. A high level of immunoreactivity was observed on gestational Days 20, 21, and 21.5 with the most significant signals found on Day 20. FP receptor protein was expressed starting on gestational Day 15, and a fluctuating... -
Expression of verocytotoxic Escherichia coli antigens in tobacco seeds and evaluation of gut immunity after oral administration in mouse model
Luciana Rossi, Alessia Di Giancamillo, Serena Reggi, Cinzia Domeneghini, Antonella Baldi, Vittorio Sala, Vittorio Dell'Orto, Ann 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 5 263-270 (8 pages)
Verocytotoxic Escherichia (E.) coli strains are responsible for swine oedema disease, which is an enterotoxaemia that causes economic losses in the pig industry. The production of a vaccine for oral administration in transgenic seeds could be an efficient system to stimulate local immunity. This study was conducted to transform tobacco plants for the seed-specific expression of antigenic proteins from a porcine verocytotoxic E. coli strain. Parameters related to an immunological response and... -
Expression of KA1 kainate receptor subunit in the substantia gelatinosa of the trigeminal subnucleus caudalis in mice
Seon Ah Park, Soo Joung Park, Seong Kyu Han* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 4 299-304 (6 pages)
The KA1 kainate receptor (KAR) subunit in the substantia gelatinosa (SG) of the trigeminal subnucleus caudalis (Vc) has been implicated in the processing of nociceptive information from the orofacial region. This study compared the expression of the KA1 KAR subunit in the SG of the Vc in juvenile, prepubescent and adult mice. RT-PCR, Western blot and immunohistochemistry analyses were used to examine the expression level in SG area. The expression levels of the KA1 KAR subunit mRNA and protein... -
Genetic characterization of Barbari goats using microsatellite markers
J. Ramamoorthi, K. Thilagam, S. N. Sivaselvam*, S. M. K. Karthickeyan 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 11 73-76 (4 pages)
Genetic variation in Barbari goats, a highly prolific breed distributed widely in the northern part of India, known for better milk and meat quality, was studied as a part of genetic characterization and conservation. The genomic DNA from 50 unrelated Barbari goats were amplified via PCR with a panel of 21 microsatellite markers, and resolved through 6 per cent denaturing polyacrylamide gel electrophoresis followed by silver staining. The number of alleles ranged from 4 to 11, with allele sizes... -
Birth of puppies after intrauterine and intratubal insemination with frozen-thawed canine semen
Hye Jin Kim, Hyun Ju Oh, Goo Jang, Min Kyu Kim 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 11 75-80 (6 pages)
The present study was performed to assess the fertility of frozen-thawed dog semen prepared by freezing with 6% glycerol and thawing at 70ºC for 8 sec, and to evaluate the least number of post-thaw spermatozoa necessary to achieve pregnancy by intrauterine or intratubal artificial insemination. It was found that the pregnancy rate of intrauterine artificial insemination was 100% using 6% glycerol buffer and thawing at 70ºC for 8 sec with 5 × 107 spermatozoa. Even though the pregnancy rate... -
Detection of Bartonella species from ticks, mites and small mammals in Korea
Chul Min Kim, Ji Young Kim, Ying Hua Yi, Mi Jin Lee, Mae rim Cho, Devendra H. Shah, Terry A. Klein, Heung Chul Kim, Jin Won Song 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 4호 11 327-334 (8 pages)
We investigated the prevalence of Bartonella infections in ticks, mites and small mammals (rodents, insectivores and weasels) collected during 2001 through 2004, from various military installations and training sites in Korea, using PCR and sequence analysis of 16S rRNA, 23S rRNA and groEL heat shock protein genes. The prevalence of Bartonella spp. was 5.2% (n = 1, 305 sample pools) in ticks, 19.1% (n = 21) in mesostigmatid mites and 13.7% (n = 424 individuals) in small mammals. The prevalence... -
Laminitis in Przewalski horses kept in a semireserve
Klaus-Dieter Budras, Klaus Scheibe, Bianca Patan, Wolf J. Streich, Kabsu Kim 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 1호 1 1-7 (7 pages)
very deep horn rings on all hooves and a laminitic horn ring on the right front hoof of one mare. An adequate laminitic therapy was not possible under the conditions of a semireserve. The applied management aimed at two goals: 1. To reduce endotoxin production and acidosis in the horses by reducing the ingestion of car-bohydrate rich food. 2. To reduce the mares level of activ-ity and to prevent tearing of the suspensory apparatus of the coffin bone. To achieve these two goals it was decided to... -
한국인 2급 1류 부정교합자의 FHI(Facial Height Index)에 영향을 미치는 요소에 관한 두부장사선 계측학적 연구
박영일(Young-il Park), 이진우(Jin-Woo Lee), 차경석(Kyung-Suk Cha) 대한치과교정학회 The Korean Journal of Orthodontics 13 Pages
대한치과교정학회 The Korean Journal of Orthodontics 1996, 제 26권 제 4호 7 401-413 (13 pages)
안모의 수직적 부조화는 안면의 전방 고경 요소와 후방 고경 요소 사이의 상관 관계에 따라 결정되는 것이므로, 전하안면 고경에 대한 후하안면 고경의 비인 FHI에 따라 수직적 control이 치료 성공의 관건인 2급 1류 부정 교합을 분류하고 FHI에 영향을 미치는 요소를 파악하여 치료의 지침으로 삼고자 본 연구를 시행하였다. 단국대학교 치과대학 교정과에 내원한 혼합치열기의 2급 1류 부정 교합자를 대조군의 FHI(평균 0.6999)의 1 S.D(0.25)를 기준으로 수직적으로 분류하였다. 임상적으로 수직적 부조화에 영향을 미칠 것으로... -
보편적 접근성 확대를 위한 블렌디드 문화예술교육사 교육과정 개발 방향 탐색 - ‘문화예술교육현장의 이해와 실습’ 과목을 중심으로 -
최은아, 임은정, 양소영, 김지현, 박천욱 한국음악교육학회 음악교육연구 21 Pages
한국음악교육학회 음악교육연구 2022, 제 51권 제 1호 7 137-157 (21 pages)
이 연구는 뉴노멀 시대에 문화예술교육의 보편적 접근성을 확보하기 위하여 문화예술교 육사 2급 자격과정 중 ‘문화예술 현장의 이해와 실습 과목’의 블렌디드 교육과정을 개발하는데 목적이 있다. 연구 방법은 ‘문화예술교육사’ 자격 과정과 ‘문화예술 현장의 이해와 실습’ 과목에 관련된 문헌을 수집 및 분석하고 코로나 팬데믹 상황으로 인한 과목 운영 실태를 파악하여 문화 예술 현장의 이해와 실습 과목의 단계별 블렌디드 교육과정 운영 방법과 내용을 도출하였다. 이러한 블렌디드 교육과정의 개발은 앞으로 다양한 사회적... -
Tricyclic antidepressant amitriptyline inhibits 5-hydroxytryptamine 3 receptor currents in NCB-20 cells
Yong Soo Park, Seok Ho Myeong, In-Beom Kim, Ki-Wug Sung 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 11 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2018, Vol.22 No.5 12 585-595 (11 pages)
Amitriptyline, a tricyclic antidepressant, is commonly used to treat depression and neuropathic pain, but its mechanism is still unclear. We tested the effect of amitriptyline on 5-hydroxytryptamine 3 (5-HT3) receptor currents and studied its blocking mechanism because the clinical applications of amitriptyline overlapped with 5-HT3 receptor therapeutic potentials. Using a whole-cell voltage clamp method, we recorded the currents of the 5-HT3 receptor when 5-HT was applied alone or co-applied... -
Effects of acidic pH on voltage-gated ion channels in rat trigeminal mesencephalic nucleus neurons
Jin-EonHan, Jin-HwaCho, In-SunChoi, Do-YeonKim, Il-SungJang 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.2 9 215-223 (9 pages)
The effects of acidic pH on several voltage-dependent ion channels, such as voltage-dependent K+ and Ca2+ channels, and hyperpolarization-gated and cyclic nucleotide-activated cation (HCN) channels, were examined using a whole-cell patch clamp technique on mechanically isolated rat mesencephalic trigeminal nucleus neurons. The application of a pH 6.5 solution had no effect on the peak amplitude of voltage-dependent K+ currents. A pH 6.0 solution slightly, but significantly inhibited the peak... -
DAMGO modulates two-pore domain K+ channels in the substantia gelatinosa neurons of rat spinal cord
PyungSunCho, HanKyuLee, , SangHoonLee, JayZoonIm, SungJunJung 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.5 10 525-531 (7 pages)
The analgesic mechanism of opioids is known to decrease the excitability of substantia gelatinosa (SG) neurons receiving the synaptic inputs from primary nociceptive afferent fiber by increasing inwardly rectifying K+ current. In this study, we examined whether a μ-opioid agonist, [D-Ala2,N-Me-Phe4, Gly5-ol]-enkephalin (DAMGO), affects the two-pore domain K+ channel (K2P) current in rat SG neurons using a slice whole-cell patch clamp technique. Also we confirmed which subtypes of K2P channels... -
Neural circuit remodeling and structural plasticity in the cortex during chronic pain
WoojinKim, SunKwangKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.1 1 1-8 (8 pages)
Damage in the periphery or spinal cord induces maladaptive plastic changes along the somatosensory nervous system from the periphery to the cortex, often leading to chronic pain. Although the role of neural circuit remodeling and structural synaptic plasticity in the ‘pain matrix’ cortices in chronic pain has been thought as a secondary epiphenomenon to altered nociceptive signaling in the spinal cord, progress in whole brain imaging studies on human patients and animal models has suggested a... -
Activation of Lysophosphatidic Acid Receptor Is Coupled to Enhancement of Ca2＋-Activated Potassium Channel Currents
Sun-HyeChoi, Byung-HwanLee, Hyeon-JoongKim, Sung-HeeHwang, Sang-MokLee, Seung-YeolNah 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2013, Vol.17 No.3 7 223-228 (6 pages)
The calcium-activated K＋ (BKCa) channel is one of the potassium-selective ion channels that are present in the nervous and vascular systems. Ca2＋ is the main regulator of BKCa channel activation. The BKCa channel contains two high affinity Ca2＋ binding sites, namely, regulators of K＋ conductance, RCK1 and the Ca2＋ bowl. Lysophosphatidic acid (LPA, 1-radyl-2-hydroxy-sn-glycero-3-phosphate) is one of the neurolipids. LPA affects diverse cellular functions on many cell types through G protein-... -
Chronic Opium Treatment Can Differentially Induce Brain and Liver Cells Apoptosis in Diabetic and Non-diabetic Male and Female Rats
MajidAsiabanha, GholamrezaAsadikaram, AmirRahnema, MehdiMahmoodi, GholamhoseinHasanshahi, MohammadHashemi6, MohammadKhaksari 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2011, Vol.15 No.6 2 327-332 (6 pages)
It has been shown that some opium derivatives promote cell death via apoptosis. This study was designed to examine the influence of opium addiction on brain and liver cells apoptosis in male and female diabetic and non-diabetic Wistar rats. This experimental study was performed on normal, opium-addicted, diabetic and diabetic opium-addicted male and female rats. Apoptosis was evaluated by TUNEL and DNA fragmentation assays. Results of this study showed that apoptosis in opium-addicted and... -
Matrix Metalloproteinase Inhibitors Attenuate Neuroinflammation Following Focal Cerebral Ischemia in Mice
CheolHongPark, TaeKyeongShin, HoYounLee, SoJungKim, WonSukLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2011, Vol.15 No.2 7 115-121 (7 pages)
The aim of this study was to investigate whether matrix metalloproteinase (MMP) inhibitors attenuate neuroinflammation in an ischemic brain following photothrombotic cortical ischemia in mice. Male C57BL/6 mice were anesthetized, and Rose Bengal was systemically administered. Permanent focal ischemia was induced in the medial frontal and somatosensory cortices by irradiating the skull with cold white light. MMP inhibitors, such as doxycycline, minocycline, and batimastat, significantly reduced... -
R-type Calcium Channel Isoform in Rat Dorsal Root Ganglion Neurons
ZhiFang, JaeHongHwang, JoongSooKim, SungJunJung, SeogBaeOh 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 5 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2010, Vol.14 No.1 7 45-49 (5 pages)
R-type Cav2.3 high voltage-activated Ca2+ channels in peripheral sensory neurons contribute to pain transmission. Recently we have demonstrated that, among the six Cav2.3 isoforms (Cav2.3a∼Cav2.3e), the Cav2.3e isoform is primarily expressed in trigeminal ganglion (TG) nociceptive neurons. In the present study, we further investigated expression patterns of Cav2.3 isoforms in the dorsal root ganglion (DRG) neurons. As in TG neurons, whole tissue RT-PCR analyses revealed the presence of two... -
Single-Channel Recording of TASK-3-like K+ Channel and Up- Regulation of TASK-3 mRNA Expression after Spinal Cord Injury in Rat Dorsal Root Ganglion Neurons
InseokJang, Jun-HoLa, Gyu-TaeKim, Jeong-SoonLee, Eun-JinKim, Eun-ShinLee, Su-JeongKim, Jeong-MinSeo, Sang-HoAhn, Jae-YongPark, Seong-GeunH 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2008, Vol.12 No.5 5 245-252 (8 pages)
Single-channel recordings of TASK-1 and TASK-3, members of two-pore domain K+ channel family, have not yet been reported in dorsal root ganglion (DRG) neurons, even though their mRNA and activity in whole-cell currents have been detected in these neurons. Here, we report single-channel kinetics of the TASK-3-like K+ channel in DRG neurons and up-regulation of TASK-3 mRNA expression in tissues isolated from animals with spinal cord injury (SCI). In DRG neurons, the single-channel conductance of... -
한국형 주정중독 선별검사 마련을 위한 예비적 연구( I )
김자성, 이철원, 김용식 대한신경정신의학회 신경정신의학 8 Pages
대한신경정신의학회 신경정신의학 1989, 제 28권 제 3호 14 500-507 (8 pages)
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랫드 척수후각 단일세포 분리 및 특성에 관한 연구
한성규(Seong Kyu Han), 류판동(Pan Dong Ryu) 대한약리학회 대한약리학잡지 10 Pages
대한약리학회 대한약리학잡지 1996, 제 32권 제 2호 15 283-292 (10 pages)
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Effects of Morphine on Somatosyrnpathetic Reflex and Arterial Blood Pressure Response Evoked by Stimulation of Peripheral Nerves
Huh, Min-Gang, Yan, Hai-Dun, Lim, Won-Il, Kim, Jun 대한생리학회 대한생리학회지 13 Pages
대한생리학회 대한생리학회지 1995, 제 29권 제 2호 13 309-321 (13 pages)
In the present study, the relationship between the somatosympathetic reflexes and arterial blood pressure responses to electrical stimulation of the peripheral nerve was investigated in cats anesthetized with α-chloralose. Single sympathetic postganglionic fiber activities were recorded from the hindlimb muscle and skin nerves and also from the cervical and abdominal sympathetic chains. Effects of the morphine on responses of the sympathetic nerve and arterial blood pressure to activation of... -
The Electrophysiological Characteristics of Medullospinal Tract Cells in Cat Ventrolateral Medulla
Lee, Woo-Yong, Kim, Sang-Jung, Kim, Jun 대한생리학회 대한생리학회지 11 Pages
대한생리학회 대한생리학회지 1991, 제 25권 제 2호 11 211-221 (11 pages)
the medullospinal tract cells in rostral and caudal medulla of cats .which branch to the lateral horn of the upper thoracic spinal cord. Cats were anesthetized with α-chloralose. The upper thoracic spinal cord and floor of the IVth ventricle were exposed. Medullospinal tract cells in rostral and caudal medulla were identified by anti-dromic stimulation of the intermediolateral nucleus in the upper thoracic cord and then the location and physiological characteristics of these cells were studied.... -
고양이 척수 전근내 감각신경 자극으로 유발된 승압반응의 생리학적 특성
김전(Kim, Jun), 서상아(Seoh, Sang-Ah), 성호경(Sung, Ho-Kyung) 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1989, 제 23권 제 1호 13 129-138 (10 pages)
척수 전근내 구심신경의 특성을 알아보기 위하여 고양이에서 L7 척수 전근을 전기자극하여 유발되는 동맥혈압의 변동을 관찰하였다. morphine을 정맥내 혹은 직접 척수에 투여하여 그 효과를 관찰하였으며 척수에 부분적인 손상을 가하여 척수 전근의 구심정보가 척수의 어느 부위를 통하여 중추로 올라가 승압반응을 유발하는지를 결정하여 다음과 같은 결과를 얻었다. 1. 척수전근을 C-강도, 높은 빈도로 자극하면 현저한 승압반응을 유발하였으며 낮은 빈도로 자극할 때에는 피부 혹은 근육감각신경을 자극할 때 보이는 감압반응이... -
Selection of Reference Genes for Real-time Quantitative PCR Normalization in the Process of Gaeumannomyces graminis var. tritici Infecting Wheat
Li-hua Xie, Xin Quan, Jie Zhang, Yan-yan Yang, Run-hong Sun, Ming-cong Xia, Bao-guo Xue, Chao Wu, Xiao-yun Han, Ya-nan Xue, Li-rong Yang 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 1호 2 11-18 (8 pages)
Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However, this method requires suitable reference genes for transcript normalization. In this study, nine candidate reference genes were chosen, and the specificity of the primers were investigated by melting curves of PCR products. The expression... -
Computational Identification and Comparative Analysis of Secreted and Transmembrane Proteins in Six Burkholderia Species
한국식물병리학회 The Plant Pathology Journal 15 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 2호 7 148-162 (15 pages)
step towards discovering novel pathogenesis-related proteins, we performed a genome scale computational identification and characterization of secreted and transmembrane (TM) proteins, which are mainly responsible for bacteria-host interactions and interactions with other bacteria, in the genomes of six representative Burkholderia species. The species comprised plant pathogens (B. glumae BGR1, B. gladioli BSR3), human pathogens (B. pseudomallei K96243, B. cepacia LO6), and plant-growth promoting... -
Development of a Rapid Detection Method for Potato virus X by Reverse Transcription Loop-Mediated Isothermal Amplification
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 3호 3 219-225 (7 pages)
The primary step for efficient control of viral diseases is the development of simple, rapid, and sensitive virus detection. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been used to detect viral RNA molecules because of its simplicity and high sensitivity for a number of viruses. RT-LAMP for the detection of Potato virus X (PVX) was developed and compared with conventional reverse transcription polymerase chain reaction (RT-PCR) to demonstrate its advantages over... -
Genetic Diversity and Differentiation of Colletotrichum spp. Isolates Associated with Leguminosae Using Multigene Loci, RAPD and ISSR
한국식물병리학회 The Plant Pathology Journal 15 Pages
한국식물병리학회 The Plant Pathology Journal 2014, 30권 1호 2 10-24 (15 pages)
Genetic diversity and differentiation of 50 Colletotrichum spp. isolates from legume crops studied through multigene loci, RAPD and ISSR analysis. DNA sequence comparisons by six genes (ITS, ACT, Tub2, CHS-1, GAPDH, and HIS3) verified species identity of C. truncatum, C. dematium and C. gloeosporiodes and identity C. capsici as a synonym of C. truncatum. Based on the matrix distance analysis of multigene sequences, the Colletotrichum species showed diverse degrees of intera and interspecific... -
A Review of Detection Methods for the Plant Viruses
한국식물병리학회 식물병연구 2014, 20권 3호 1 173-181 (9 pages)
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An Expected Crisis : A Case Study of Volkswagen Diesel Crisis
Sang Yeal Lee, Hong min Ahn, Yong Suk Cho 한국광고PR실학회 광고PR실학연구 21 Pages
한국광고PR실학회 광고PR실학연구 2017, 제 10권 1호 10 244-264 (21 pages)
This exploratory case study analyzes the Volkswagen diesel crisis from a crisis communication perspective. The Volkswagen diesel case provides a unique opportunity to examine how a Fortune 500 company managed (or mismanaged) an intentional corporate misdeed, and to understand the shortcomings of existing crisis communication theories. In analyzing the case, this study adopted the theoretical frameworks from the two dominant crisis communication theories, namely Image Repair Theory and... -
An Analysis of Attitudes, Perceptions, and Practices of Shared Reading among Preschool Teachers in Malaysia
Affizal Ahmad;Wan Yi Low;Hui Min Low 환태평양유아교육연구학회 Asia-Pacific journal of research in early childhood education 22 Pages
환태평양유아교육연구학회 Asia-Pacific journal of research in early childhood education 2018, Vol.12 No.3 2 23-44 (22 pages)
Shared reading is a common instructional activity in preschools. However, not all preschool teachers receive training in shared reading. This research investigated factors that influenced the attitudes, perceptions, and practices of shared reading among an urban sample of preschool teachers in Malaysia. The findings revealed that prior training experience in shared reading courses or seminars significantly influenced preschool teachers’ attitudes toward shared reading. Those who reportedly had... -
How Do Children Learn? Beliefs and Practices Reported by Kindergarten Teachers in Singapore
Ching Ting Tan, Nirmala Rao 환태평양유아교육연구학회 Asia-Pacific journal of research in early childhood education 32 Pages
환태평양유아교육연구학회 Asia-Pacific journal of research in early childhood education 2017, Vol.11 No.3 5 81-112 (32 pages)
This study explored the curriculum-related beliefs and practices reported by Singapore kindergarten teachers at a time when concerted efforts are being made to promote children’s holistic development through a child-centered curriculum that emphasizes active learning through play and interaction. A questionnaire was administered to a sample of 167 teachers of 4- to 5-year-olds. Factor analyses revealed three reliable factors for teachers’ beliefs and four factors for their classroom practices.... -
앞으로 걷기와 뒤로 걷기 시 에너지 소비량과 피로도 비교
김민정, 신윤아, 박광동, 장석암, 옥정석, 석민화, 임강일, 류현승 한국웰니스학회 한국웰니스학회지 8 Pages
한국웰니스학회 한국웰니스학회지 2010, 제 5권 2호 6 67-74 (8 pages)
걷기운동은 신체 대근육군의 율동적이고 역동적인 움직임을 통해 이루어지는 운동으로 신체에 부작용을 거의 주지 않고 쉽게 반복할 수 있어 신체활동량을 증가시킬 수 있는 활용도가 높은 운동방법이다(김영혜와 양영옥, 2005; Morris & Hardman, 1997). 또한 걷기는 특별한 도구나 장비 없이도 할 수 있는 유산소운동으로서, 다른 운동에 비해 지속율이 높고(정정욱과 김훈, 2004), 몸에 무리를 주지 않으면서 건강관련 위험요인을 개선시키는 효과적이고 안전한 운동으로 권장되고 있다(남상남, 2005; Tudor-Locke & Bassett, 2001).... -
Graphene: an emerging material for biological tissue engineering
SangKyu Lee, Hyun Kim, BongSup Shim 한국탄소학회 Carbon Letters 13 Pages
한국탄소학회 Carbon Letters 2013, Vol.14 No.2 1 63-75 (13 pages)
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Identifying and quantitating defects on chemical vapor de-position grown graphene layers by selected electrochemical deposition of Au nanoparticles
Hye-Mi So, Jeong Hun Mun, Gyeong Sook Bang, Taek Yong Kim, Byung Jin Cho, Chi Won Ahn 한국탄소학회 Carbon Letters 4 Pages
한국탄소학회 Carbon Letters 2012, Vol.13 No.1 10 56-59 (4 pages)
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Polyphenism by the level of predation risk in larval salamander, Hynobius leechii
Jihee Hwnag, Eun-ji Kim, Ho-Jin Kim, Hoon Chung 한국습지학회 한국습지학회지 7 Pages
한국습지학회 한국습지학회지 2013, Vol.15 No.4 7 485-491 (7 pages)
This study examined the cannibalistic polyphenism of larval salamander Hynobius leechii by the level of predation risk. Salamander eggs were collected from three regions (Mountain Inwang, Mountain Surak and Gwangju). Eggs were treated by three different risk conditions: (1) high risk, predation risk three times a day; (2) medium risk, predation risk once a day; and (3) low risk, no predation risk. Predation risk was conducted using a chemical cue from Chinese minnows. The chemical cue treatment... -
자기주도적 교수 역량 강화를 위한 PDS 모형 개발에 관한 연구
구원회, 박영희, 나귀수, 황연주, 하정미 이화여자대학교 교과교육연구소 교과교육학연구 21 Pages
이화여자대학교 교과교육연구소 교과교육학연구 2010, 제 14권 3호 7 579-599 (21 pages)
이 논문의 목적은 교사 전문성 발달의 새로운 접근으로 주목받고 있는 PDS(Professional Development System)의 관점에서 한국의 교사들이 자기주도적인 교수 역량을 강화할 수 있는 PDS 모형을 개발하고, 개발한 PDS 모형의 현실 적합성을 검토하는 것이다. 연구진은 국내외 관련 연구 에 대한 검토, 설문조사, 학술대회 등를 통해 PDS 모형을 탐색하였고, PDS의 다섯 가지 기준을 협동, 학습공동체, 구조와 자원, 자기 주도성, 평가로 설정하고, 교사공동체 중심, 연수 중심, 단 위학교 중심의 PDS 모형을 개발하였다. 이러한 PDS... -
An Approach to Increase Intrinsic Motivation in Physical Activity
유생열 경인교육대학교 교육연구원 교육논총 20 Pages
경인교육대학교 교육연구원 교육논총 2006, 제 26권 제 1호 2 19-38 (20 pages)
교육과 스포츠 및 운동 심리학 분야에서 내적동기와 관련된 많은 연구는 개인이 내 적으로나 외적으로 특정한 성취활동에 동기가 유발되는 요인을 규명하는데 노력해왔 다. 여기서 내적동기 성향이란 어떤 성취활동에 참가한 개인이 내적인 이유, 예를 들 어 즐거움, 재미, 그리고 개인의 숙달과 같은 활동에 참여하는 것을 말한다. 반면에 외적 동기 성향이란 개인이 외적인 이유, 예를 들어 사회적인 승인, 사회적 지위, 물 질의 보상과 같은 이유 때문에 성취활동에 참여하는 개인을 말하고 있다. Deci와 Ryan (1985)의 인지평가... -
초음파 용접용 실린더 혼의 설계
김선락, 이재학, 유중돈, Kim. Sun-Rak, Lee. Jae-Hak, Yoo. Choong-D. 대한용접접합학회 大韓溶接·接合學會誌 7 Pages
대한용접접합학회 大韓溶接·接合學會誌 2009, Vol.27 No.4 60-66 (7 pages)
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Dehydroevodiamine·HCl enhances cognitive function in memory-impaired rat models
KiYoungShin, KaYoungKim, Yoo-HunSuh 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.1 7 55-64 (10 pages)
Progressive memory impairment such as that associated with depression, stroke, and Alzheimer’s disease (AD) can interfere with daily life. In particular, AD, which is a progressive neurodegenerative disorder, prominently features a memory and learning impairment that is related to changes in acetylcholine and abnormal β-amyloid (Aβ) deposition in the brain. In the present study, we investigated the effects of dehydroevodiamine·HCl (DHED) on cognitive improvement and the related mechanism in... -
Purification and Characterization of a Major Extracellular Chitinase from a Biocontrol Bacterium, Paenibacillus elgii HOA73
한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 3호 10 318-328 (11 pages)
Chitinase-producing Paenibacillus elgii strain HOA73 has been used to control plant diseases. However, the antimicrobial activity of its extracellular chitinase has not been fully elucidated. The major extracellular chitinase gene (PeChi68) from strain HOA73 was cloned and expressed in Escherichia coli in this study. This gene had an open reading frame of 2,028 bp, encoding a protein of 675 amino acid residues containing a secretion signal peptide, a chitin-binding domain, two fibronectin type... -
Efficacy of Tissue Culture in Virus Elimination from Caprifig and Female Fig Varieties (Ficus carica L.)
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 3호 7 288-295 (8 pages)
Fig mosaic disease (FMD) is a viral disease that spreads in all Tunisian fig (Ficus carica L.) orchards. RT-PCR technique was applied to leaf samples of 29 fig accessions of 15 fig varieties from the fig germplasm collection of High Agronomic Institute (I.S.A) of Chatt-Mariem, to detect viruses associated to FMD. Analysis results show that 65.5% of the accessions (19/29) and 80.0% (12/15) of the fig varieties are infected by FMD-associated viruses. From all fig accessions, 41.4% of them are with... -
Preschool Inclusion Placement in Taiwan
Shu-hsien Tseng 환태평양유아교육연구학회 Asia-Pacific journal of research in early childhood education 23 Pages
환태평양유아교육연구학회 Asia-Pacific journal of research in early childhood education 2018, Vol.12 No.3 4 69-91 (23 pages)
Despite the steady decline in birth rate in Taiwan, the number of special education children continues to increase, especially after the implementation of the Early Childhood Education and Care Act in 2012. The most significant increase was seen with placing special education preschoolers in inclusion classrooms. In particular, the number of students receiving itinerant services has grown the most dramatically, accompanied by a decrease in the number of students receiving special education...
