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전체 선택해제
총 167건 검색
본문검색 결과만 보기
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An Effective Data Analysis System for Improving Throughput of Shotgun Proteomic Data based on Machine Learning
나승진, 백은옥, Na. Seung-Jin, Paek. Eun-Ok 한국정보과학회 정보과학회논문지. Journal of KIISE. 소프트웨어 및 응용 11 Pages
한국정보과학회 정보과학회논문지. Journal of KIISE. 소프트웨어 및 응용 2007, Vol.34 No.10 889-899 (11 pages)
최근 프로테오믹스 분야에서 단백질의 추출, 분리기술의 발전과 고성능 질량분석 장비로 인하여 대량으로, 또 빠르게 샘플을 분석하는 것이 가능해짐에 따라서, 한번의 실험으로부터 얻어지는 실험데이타의 양이 대폭 늘어나게 되었다. 따라서 대량의 데이타를 어떻게 처리하여 필요한 정보만을 얻어내는가가 큰 이슈가 되고 있다. 하지만 기존의 데이타 해석과정은 불필요하게 계산자원을 낭비하는 요소를 상당 부분을 포함하고 있고, 이로 인해 데이타 해석 시간이 증가함은 물론, 종종 옳지 않은 해석 결과를 생성함으로써 결과에 대한... -
HMM-based Adaptive Frequency-Hopping Cognitive Radio System to Reduce Interference Time and to Improve Throughput
Sohn. Sung-Hwan, Jang. Sung-Jeen, Kim. Jae-Moung 한국인터넷정보학회 KSII Transactions on internet and information systems : TIIS 16 Pages
한국인터넷정보학회 KSII Transactions on internet and information systems : TIIS 2010, Vol.4 No.4 475-490 (16 pages)
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Erratum to "Microfluidic System Based High Throughput Drug Screening System for Curcumin/TRAIL Combinational Chemotherapy in Human Prostate Cancer PC3 Cells" [Biomol. Ther. 22 (2014) 355-362]
An. Dami, Kim. Kwangmi, Kim. Jeongyun 한국응용약물학회 Biomolecules & therapeutics 1 Pages
한국응용약물학회 Biomolecules & therapeutics 2014, Vol.22 No.5 475-475 (1 pages)
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Microfluidic System Based High Throughput Drug Screening System for Curcumin/TRAIL Combinational Chemotherapy in Human Prostate Cancer PC3 Cells
An. Dami, Kim. Kwangmi, Kim. Jeongyun 한국응용약물학회 Biomolecules & therapeutics 8 Pages
한국응용약물학회 Biomolecules & therapeutics 2014, Vol.22 No.4 355-362 (8 pages)
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Development of a FRET-based High-Throughput Screening System for the Discovery of Hsp90 Inhibitors
Oh. Sang-Mi, Ko. Yeon-Jin, Lee. Han-Jae, Kim. Jong-Hoon, Chung. Young-Sun, Park. Seung-Bum 대한화학회 Bulletin of the Korean Chemical Society 4 Pages
대한화학회 Bulletin of the Korean Chemical Society 2011, Vol.32 No.9 3229-3232 (4 pages)
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Throughput of V-BLAST System using Hybrid ARQ in Correlated Fading Channels
김봉회, 황승훈, Kim. Bong-Hoe, Hwang. Seung-Hoon 대한전자공학회 電子工學會論文誌. Journal of the Institute of Electronics Engineers of Korea. TC, 통신 6 Pages
대한전자공학회 電子工學會論文誌. Journal of the Institute of Electronics Engineers of Korea. TC, 통신 2008, Vol.45 No.1 66-71 (6 pages)
본 논문에서는 H-ARQ를 위한 NACK 신호를 이용한 안테나 스위칭 방식을 제안하고 V-BLAST 시스템에서 분석하였다. 여기서 전송 안테나는 재전송시 번갈아 스위칭 되어 동작한다. 상관된 페이딩 채널에서 제안된 방식의 채널 용량을 해석적으로 분석함으로써, 채널 상관성의 악영향이 안테나 스위칭 방식으로 줄어듦을 보인다. 시뮬레이션 결과를 통해 상관된 페이딩 채널에서 평균 수율은 큰 복잡도의 증가 없이 개선되어질 수 있음을 보인다. -
Establishment of High Throughput Screening System Using Human Umbilical Cord-derived Mesenchymal Stem Cells
Park. Eu-Gene, Cho. Tae-Jun, Oh. Keun-Hee, Kwon. Soon-Keun, Lee. Dong-Sup, Park. Seung-Bum, Cho. Jae-Jin 대한구강생물학회 International journal of oral biology : official journal of the Korean Academy of Oral Biology and t 8 Pages
대한구강생물학회 International journal of oral biology : official journal of the Korean Academy of Oral Biology and t 2012, Vol.37 No.2 43-50 (8 pages)
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The Use of a Tobacco mosaic virus-Based Expression Vector System in Chrysanthemum
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 4호 9 429-433 (5 pages)
Chrysanthemums (Chrysanthemum morifolium) are susceptible to tobacco mosaic virus (TMV). TMV-based expression vectors have been used in high-throughput experiments for production of foreign protein in plants and also expressing green fluorescent protein (GFP) to allow visualization of TMV movement. Here, we used TMV expressing the GFP to examine the infection of chrysanthemum by a TMV-based expression vector. Viral replication, movement and GFP expression by TMV-GFP were verified in upper leaves... -
Melting Characteristics of Simulated Non-combustible Radioactive Wastes in a Plasma Torch Melter System
Seung-Chul Park, Hyun-Je Cho, Cheon-Woo Kim 한국열환경공학회 열환경공학 7 Pages
한국열환경공학회 열환경공학 2011, 제 8권 3호 7 53-59 (7 pages)
with the invention of the KHNP-CRI patented power supply system. Among a series of dual mode performance tests, a 24-hour melting test for typical surrogate non-combustible waste is introduced in this paper. Electrical currents and voltages of the system was well confined under the operation limits at the test. And the slag melt was well stabilized without severe abnormal behavior as well. But for safe and long-run operation, the advanced slag tapping equipment and slag level measurement device... -
High Throughput Screening of Antifungal Metabolites Against Colletotrichum gloeosporioides
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2008, 24권 1호 4 24-30 (7 pages)
Inhibition of appressorium formation by culture filtrate of A5005 was partially restored by the exogenous calcium. This results suggests that chemicals within A5005 extents its biological activity through disturbance of intracellular Ca2+ regulation during prepenetration morphogenesis by C. gloeosporioides. Together, cell-based and target-oriented screening system used in this study should be applicable for other plant pathogenic fungi prerequisite appressorium formation to infect their hosts. -
Evolved Packet System의 3G LTE에서 패킷별 지연허용시간을 고려한 AMC 선택 기법
전경구, Jun. Kyung-Koo 한국통신학회 한국통신학회논문지. The journal of Korea Information and Communications Society. 무선통신 7 Pages
한국통신학회 한국통신학회논문지. The journal of Korea Information and Communications Society. 무선통신 2008, Vol.33 787-793 (7 pages)
요소로 보고, 패킷별로 다른 최대 패킷전송 오류 확률 $P_{max}$를 적용하여 AMC 전송 모드를 선정하는 기준으로 삼는다. 이러한 $P_{max}$를 결정할 때는 채널 상황은 물론 패킷별 QoS 요구상황, 그리고 NACK-to-ACK 에러 확률을 고려하여 결정한다. 이러한 방식의 목적은 QoS를 만족시키면서 동시에 spectral efficiency를 최대로 하는 것이다. 시뮬레이션을 통해 제안한 방법과 기존 방법을 비교한 결과, 지연허용 시간 위반과 재전송과 관련깊은 status report 발생은 10% 감소하고, throughput이 12% 증가했음을 확인할 수 있었다. -
Analysis of Interference Impact of UWB System on WiBro System
윤영근, 홍헌진, 이일규, 최익권, 곽경섭, Yoon. Young-Keun, Hong. Heon-Jin, Lee. Il-Kyoo, Choi. Ik-Guen, Kwak. Kyung-Sub 한국통신학회 한국통신학회논문지. The journal of Korea Information and Communications Society. 무선통신 6 Pages
한국통신학회 한국통신학회논문지. The journal of Korea Information and Communications Society. 무선통신 2006, Vol.31 814-819 (6 pages)
상황을 고려하여 단일 UWB 간섭의 경우는 최소결합손실(MCL) 방법으로 간섭분석을 수행하였고, 다중 UWB 간섭인 경우는 실제 상황을 고려한 간섭 시나리오를 바탕으로 몬테칼로(MC) 방법으로 간섭 시뮬레이션을 수행하였다. 분석 및 시뮬레이션 결과 두 시스템의 양립성을 보장하기 위해 단일 간섭 원 과 희생(victim) 수신기 사이의 이격 거리는 34.4m 이상이 요구되었다. 또한, 다중 UWB 간섭 원 인 경우에 WiBro 단말기의 최소 throughput 손실을 보장하기 위해 허용할 수 있는 UWB 송신전력 레벨은 약 - 81 dBm / MHz 이하로... -
Optimization of Expression Conditions for Soluble Protein by Using a Robotic System of Multi-culture Vessels
Ahn. Woo-Sung, Ahn. Ji-Young, Jung. Chan-Hun, Hwang. Kwang-Yeon, Kim. Eunice Eun-Kyeong, Kim. Joon, Im. Ha-Na, Kim. Jin-Oh, Yu. Myeong-H 한국미생물생명공학회 Journal of microbiology and biotechnology 7 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2007, Vol.17 No.11 1868-1874 (7 pages)
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Identification of Inhibitors Against BAK Pore Formation using an Improved in vitro Assay System
Song. Seong-Soo, Lee. Won-Kyu, Aluvila. Sreevidya, Oh. Kyoung Joon, Yu. Yeon Gyu 대한화학회 Bulletin of the Korean Chemical Society 6 Pages
대한화학회 Bulletin of the Korean Chemical Society 2014, Vol.35 No.2 419-424 (6 pages)
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Genome-Wide Analysis of Type VI System Clusters and Effectors in Burkholderia Species
한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 1호 2 11-22 (12 pages)
secretion system (T6SS) has been discovered in a variety of gram-negative bacteria as a versatile weapon to stimulate the killing of eukaryotic cells or prokaryotic competitors. Type VI secretion effectors (T6SEs) are well known as key virulence factors for important pathogenic bacteria. In many Burkholderia species, T6SS has evolved as the most complicated secretion pathway with distinguished types to translocate diverse T6SEs, suggesting their essential roles in this genus. Here we attempted... -
Construction of a System for the Strawberry Nursery Production towards Elimination of Latent Infection of Anthracnose Fungi by a Combination of PCR and Microtube Hybridization
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 1호 9 80-86 (7 pages)
from fungal hyphae. For specific detection of the fungi, PCR primers were designed to amplify the fungal MAT1-2 gene. The subsequent MTH step using the PCR products as probes can replace the laborious electrophoresis step providing us sequence information and high-throughput screening. Using this method, we have conducted a survey for a few thousands nursery plants every year for three consecutive years, and finally succeeded in eliminating latent infection in the third year of challenge. -
Research Notes : Estrogenic Compounds Compatible with a Conditional Gene Expression System for the Phytopathogenic Fungus Fusarium graminearum
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 4호 7 349-353 (5 pages)
in this system. Among them, β-estradiol was able to successfully induce the expression of a gene controlled by Pzear, while estrone was only able to partially induce its expression but the other two compounds were not effective. In combination, these results demonstrate that β-estradiol can replace ZEA in this conditional gene expression system, thereby eliminating the need to use the more expensive reagent, ZEA, and facilitating high-throughput functional analyses of F. graminearum in future... -
Generation of Expression Vectors for High-Throughput Functional Analysis of Target Genes in Schizosaccharomyces pombe
Ahn. Ji-Won, Choi. Chung-Hae, Kang. Chang-Mo, Kim. Chun-Ho, Park. Hee-Moon, Song. Kyung-Bin, Hoe. Kwang-Lae, Won. Mi-Sun, Chung. Kyung-S 한국미생물학회 The journal of microbiology 7 Pages
한국미생물학회 The journal of microbiology 2009, Vol.47 No.6 789-795 (7 pages)
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Molecular-level evaluation of selected periodontal pathogens from subgingival regions in canines and humans with periodontal disease
Magdalena Goły?ska, Izabela Polkowska, Małgorzata Bartoszcze-Tomaszewska, Aleksandra Sobczy?ska-Rak, Łukasz Matuszewski 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 1호 7 51-58 (8 pages)
Dogs commonly serve as a model for various human conditions, including periodontal diseases. The aim of this study was to identify the anaerobic bacteria that colonize the subgingival areas in dogs and humans by using rapid real-time polymerase chain reaction (RT-PCR)-based tests and to compare the results obtained in each species. Bacterial microflora evaluations, both quantitative and qualitative, were performed by applying ready-made tests on twelve dogs and twelve humans. Five samples were... -
Quantitative real-time polymerase chain reaction for detecting Mycoplasma hyosynoviae and Mycoplasma hyorhinis in pen-based oral, tonsillar, and nasal fluids
João Carlos Gomes Neto, Leslie Bower, Barbara Z. Erickson, Chong Wang, Matthew Raymond, Erin L. Strait 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 9 195-201 (7 pages)
Mycoplasma (M.) hyorhinis and M. hyosynoviae are pathogens known to cause disease in pigs post-weaning. Due to their fastidious nature, there is increased need for culture-independent diagnostic platforms to detect these microorganisms. Therefore, this study was performed to develop and optimize quantitative real-time PCR (qPCR) assays to rapidly detect M. hyorhinis and M. hyosynoviae in pen-based oral fluids as well as nasal and tonsillar fluids as proxies for samples used in swine herd... -
An overview of calf diarrhea - infectious etiology, diagnosis, and intervention
Yong-il Cho, Kyoung-Jin Yoon* 대한수의학회 Journal of Veterinary Science 17 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 1 1-17 (17 pages)
of the 2007 National Animal Health Monitoring System for U.S. dairy, half of the deaths among unweaned calves was attributed to diarrhea. Multiple pathogens are known or postulated to cause or contribute to calf diarrhea development. Other factors including both the environment and management practices influence disease severity or outcomes. The multifactorial nature of calf diarrhea makes this disease hard to control effectively in modern cow-calf operations. The purpose of this review is to... -
Sporozoite proteome analysis of Cryptosporidium parvum by one-dimensional SDS-PAGE and liquid chromatography tandem mass spectrometry
AMAM Zonaed Siddiki* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 1 107-114 (8 pages)
Despite the development of new technologies, new challenges still remain for large scale proteomic profiling when dealing with complex biological mixtures. Fractionation prior to liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis is usually the preferred method to reduce the complexity of any biological sample. In this study, a gel LC-MS/MS approach was used to explore the stage specific proteome of Cryptosporidium (C.) parvum. To accomplish this, the sporozoite protein of C.... -
Plasma haptoglobin and immunoglobulins as diagnostic indicators of deoxynivalenol intoxication
Eun-Joo Kim, Sang-Hee Jeong, Joon-Hyoung Cho, Hyun-Ok Ku, Hyun-Mi Pyo, Hwan-Goo Kang, Kyoung-Ho Choi 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 3호 5 257-266 (10 pages)
This study aimed to discover potential biomarkers for dioxynivalenol (DON) intoxication. B6C3F1 male mice were orally exposed to 0.83, 2.5 and 7.5 mg/kg body weight (bw) DON for 8 days and the differential protein expressions in their blood plasma were determined by SELDI - Time-of-Flight/ Mass Spectrometry (TOF/MS) and the immunoglobulins (Igs) G, A, M and E in the serum were investigated. 11.7 kDa protein was significantly highly expressed according to DON administration and this protein was... -
Validation of an electrometric blood cholinesterase measurement in goats
M. M.H. Al-Jobory, F. K. Mohammad 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 4호 7 299-303 (5 pages)
A modified electrometric method was described and validated for measurement of plasma and erythrocyte cholinesterase activities in 6~18 months old goats. The enzymatic reaction mixture contained 3 ml distilled water, 3 ml barbital-phosphate buffer (pH 8.1), 0.2 ml plasma or erythrocytes and 0.1 ml acetylthiocholine iodide (7.5%) as a substrate. The mixture was incubated at 37 degrees C for 40 minutes. The pH of the reaction mixture was determined by a pH meter before and after the incubation.... -
Development of a Lightcycler-based reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus
Jae Ku Oem, Soo Jeong Kye, Kwang Nyeong Lee, Yong Joo Kim, Jee Yong Park, Jong Hyeon Park, Yi Seok Joo, Hee Jong Song 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 3호 6 207-212 (6 pages)
One step TaqMan real-time reverse transcription polymerase chain reaction (R/T RT-PCR)using a set of primers/probes was developed for the detection of foot-and-mouth disease (FMD)virus. The gene-specific probes labeled fluorogen for the internal ribosomal entry site, Leader sequence and 2B regions were used to detect FMD virus (FMDV). This assay specifically detected FMDV both in cell culture preparations and clinical samples, and was capable of distinguishing FMD from other viral diseases... -
Application of biotechnological tools for coccidia vaccine development
Wongi Min, Rami A. Dalloul, Hyun S. Lillehoj 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 1 279-288 (10 pages)
Coccidiosis is a ubiquitous intestinal protozoan infection of poultry seriously impairing the growth and feed utilization of infected animals. Conventional disease control strategies have relied on prophylactic medication. Due to the continual emergence of drug resistant parasites in the field and increasing incidence of broiler condemnations due to coccidia, novel approaches are urgently needed to reduce economic losses. Understanding the basic biology of host-parasite interactions and... -
Bacterial diversity and its relationship to growth performance of broilers
Yeonji Bae , Bonsang Koo , Seungbaek Lee , Jongsuk Mo , Kwanghyun Oh , In Pil Mo 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 9 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2017, 제 57권 제 3호 3 159-167 (9 pages)
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유아교사의 국악교육에 대한 인식과 지도능력 및 현황에 관한 실태조사
송주승(JuSeung Song), 정혜명(HyeMyoung Choung) 한국음악교육공학회 음악교육공학 15 Pages
한국음악교육공학회 음악교육공학 2014, 제 18호 7 119-133 (15 pages)
본 연구는 유아교육기관에 근무하고 있는 교사들의 국악교육에 대한 인식과 지도능력, 그리고 적용현황을 알아보는데 목적을 두었다. 인천광역시에 소재한 공. 사립유치원과 어린이집에 재직하고 있는 교사들 90명에게 설문지를 배부하였다. 회수된 73명 중에 응답이 완성되지 않았거나 잘못 표기된 13부는 제외하고 60부를 최종 분석 하였다. 연구 분석결과에 의하면 교사들이 유아국악교육의 필요함을 인식하고 있으나 교사들의 기초적인 실기능력과 지식 등의 지도능력이 부족하여 활동이 원활하지 않거나 부담을 느끼는 교사들이... -
High fat diet confers vascular hyper-contractility against angiotensin II through upregulation of MLCK and CPI-17
JeeInKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.1 12 99-106 (8 pages)
Obesity is a critical risk factor for the hypertension. Although angiotensin II (Ang II) in obese individuals is known to be upregulated in obesity-induced hypertension, direct evidence that explains the underlying mechanism for increased vascular tone and consequent increase in blood pressure (BP) is largely unknown. The purpose of this study is to investigate the novel mechanism underlying Ang II-induced hyper-contractility and hypertension in obese rats. Eight-week old male Sprague-Dawley... -
Cryptotanshinone inhibits TNF-α-induced LOX-1 expression by suppressing reactive oxygen species (ROS) formation in endothelial cells
XiaoliRan, WenwenZhao, WenpingLi, JingshanShi, XiupingChen 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.4 4 347-355 (9 pages)
Cryptotanshinone (CPT) is a natural compound isolated from traditional Chinese medicine Salvia miltiorrhiza Bunge. In the present study, the regulatory effect and potential mechanisms of CPT on tumor necrosis factor alpha (TNF-α) induced lectin-like receptor for oxidized low density lipoprotein (LOX-1) were investigated. Human umbilical vein endothelial cells (HUVECs) were cultured and the effect of TNF-α on LOX-1 expression at mRNA and protein levels was determined by Real-time PCR and... -
Stem Cells in Drug Screening for Neurodegenerative Disease
Hyun-JungKim, ChangYunJin 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2012, Vol.16 No.1 1 1-9 (9 pages)
Because the average human life span has recently increased, the number of patients who are diagnosed with neurodegenerative diseases has escalated. Recent advances in stem cell research have given us access to unlimited numbers of multi-potent or pluripotent cells for screening for new drugs for neurodegenerative diseases. Neural stem cells (NSCs) are a good model with which to screen effective drugs that increase neurogenesis. Recent technologies for human embryonic stem cells (ESCs) or induced... -
Modern Methods for Analysis of Antiepileptic Drugs in the Biological Fluids for Pharmacokinetics, Bioequivalence and Therapeutic Drug Monitoring
JuseopKang, Yoo-SinPark, Shin-HeeKim, Sang-HyunKim, Min-YoungJun 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 16 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2011, Vol.15 No.2 1 67-82 (16 pages)
Epilepsy is a chronic disease occurring in approximately 1.0% of the world's population. About 30% of the epileptic patients treated with availably antiepileptic drugs (AEDs) continue to have seizures and are considered therapy-resistant or refractory patients. The ultimate goal for the use of AEDs is complete cessation of seizures without side effects. Because of a narrow therapeutic index of AEDs, a complete understanding of its clinical pharmacokinetics is essential for understanding of the... -
Effect of Bacillus mesonae H20-5 Treatment on Rhizospheric Bacterial Community of Tomato Plants under Salinity Stress
Shin Ae Lee, Hyeon Su Kim, Mee Kyung Sang, Jaekyeong Song, Hang-Yeon Weon 한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 6호 15 662-671 (10 pages)
Plant growth-promoting bacteria improve plant growth under abiotic stress conditions. However, their effects on microbial succession in the rhizosphere are poorly understood. In this study, the inoculants of Bacillus mesonae strain H20-5 were administered to tomato plants grown in soils with different salinity levels (EC of 2, 4, and 6 dS/m). The bacterial communities in the bulk and rhizosphere soils were examined 14 days after H20-5 treatment using Illumina MiSeq sequencing of the bacterial... -
Whole-Genome Characterization of Alfalfa Mosaic Virus Obtained from Metagenomic Analysis of Vinca minor and Wisteria sinensis in Iran: with Implications for the Genetic Structure of the Virus
Zohreh Moradi, Mohsen Mehrvar 한국식물병리학회 The Plant Pathology Journal 13 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 6호 11 619-631 (13 pages)
Alfalfa mosaic virus (AMV), an economically important pathogen, is present worldwide with a very wide host range. This work reports for the first time the infection of Vinca minor and Wisteria sinensis with AMV using RNA sequencing and reverse transcription polymerase chain reaction confirmation. De novo assembly and annotating of contigs revealed that RNA1, RNA2, and RNA3 genomic fragments consist of 3,690, 2,636, and 2,057 nucleotides (nt) for IR-VM and 3,690, 2,594, and 2,057 nt for IR-WS.... -
Detection of Blackleg Resistance Gene Rlm1 in Double-Low Rapeseed Accessions from Sichuan Province, by Kompetitive Allele-Specific PCR
Liang Chai, Jinfang Zhang, Wannakuwattewaduge Gerard Dilantha Fernando, Haojie Li, Xiaoqin Huang, Cheng Cui, Jun Jiang, Benchuan Zheng, Yong Liu, Liangcai Jiang 한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 2호 10 194-199 (6 pages)
Blackleg is a serious disease in Brassica plants, causing moderate to severe yield losses in rapeseed worldwide. Although China has not suffered from this disease yet (more aggressive Leptosphaeria maculans is not present yet), it is crucial to take provisions in breeding for dis- ease resistance to have excellent blackleg-resistant cul- tivars already in the fields or in the breeding pipeline. The most efficient strategy for controlling this disease is breeding plants with identified resistance... -
Cucumber Mosaic Virus 1a Protein Interacts with the Tobacco SHE1 Transcription Factor and Partitions between the Nucleus and the Tonoplast Membrane
Ju-Yeon Yoon, Peter Palukaitis 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 2호 9 182-193 (12 pages)
the yeast two-hybrid system, by a pull-down assay, and via bimolecular fluorescent complementation. Using fluorescent-tagged proteins and confocal microscopy, the CMV 1a protein itself was found distributed predominantly between the nu- cleus and the tonoplast membrane, although it was also found in speckles in the cytoplasm. The SHE1 protein was localized in the nucleus, but in the presence of the CMV 1a protein was partitioned between the nucleus and the tonoplast membrane. SHE1 expression was... -
Necrotrophic Fungus Pyrenophora tritici-repentis Triggers Expression of Multiple Resistance Components in Resistant and Susceptible Wheat Cultivars
Ethan J. Andersen, Madhav P. Nepal, Shaukat Ali 한국식물병리학회 The Plant Pathology Journal 16 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 2호 2 99-114 (16 pages)
Tan spot of wheat, caused by Pyrenophora tritici-re- pentis (Ptr), results in a yield loss through chlorosis and necrosis of healthy leaf tissue. The major objective of this study was to compare gene expression in resistant and susceptible wheat cultivars after infection with Ptr ToxA-producing race 2 and direct infiltration with Ptr ToxA proteins. Greenhouse experiments included ex- posure of the wheat cultivars to pathogen inoculum or direct infiltration of leaf tissue with Ptr-ToxA protein... -
Antiserum Preparation of Recombinant Sweet Potato Latent Virus-Lotus (SPLV-Lotus) Coat Protein and Application for Virus-Infected Lotus Plant Detection
Zhen He, Tingting Dong, Wen Chen, Tielin Wang, Haifeng Gan, LiangJun Li 한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 6호 13 651-657 (7 pages)
in lotus planting area were developed. The established ELISA and dot blot diagnostic methods can be used to detect SPLVlotus from samples successfully. And our results also showed that the SPLV-lotus and sweet potato isolates appeared clearly distinction in serology. Our study provides a high-throughput, sensitive, and rapid diagnostic method based on serology that can detect SPLV on lotus, which is suggested to be included in viral disease management approach due to its good detection level. -
Characterization of Hibiscus Latent Fort Pierce Virus-Derived siRNAs in Infected Hibiscus rosa-sinensis in China
Han-hong Lan, Luan-mei Lu 한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 6호 9 618-627 (10 pages)
Although limited progress have been made about pathogen system of Hibiscus rosa-sinensis and Hibiscus latent Fort Pierce virus (HLFPV), interaction between plant host and pathogen remain largely unknown, which led to deficiency of effective measures to control disease of hibiscus plants caused by HLFPV. In this study, infection of HLFPV in Hibiscus rosa-sinensis was firstly confirmed for the first time by traditional electron microscopy, modern reverse transcription polymerase chain reaction and... -
Assessment of the Contribution of Antagonistic Secondary Metabolites to the Antifungal and Biocontrol Activities of Pseudomonas fluorescens NBC275
Swarnalee Dutta, Sang-Mi Yu, Yong Hoon Lee 한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 5호 11 491-496 (6 pages)
An understanding of the contribution of secondary metabolites (SMs) to the antagonistic and biocontrol activities of bacterial biocontrol agents serves to improve biocontrol potential of the strain. In this study, to evaluate the contribution of each SM produced by Pseudomonas fluorescens NBC275 (Pf275) to its antifungal and biocontrol activity, we combined in silico analysis of the genome with our previous study of transposon (Tn) mutants. Thirteen Tn mutants, which belonged to 6 biosynthetic... -
Rapid and Efficient Detection of 16SrI Group Areca Palm Yellow Leaf Phytoplasma in China by Loop-Mediated Isothermal Amplification
Shao-shuai Yu, Hai-yan Che, Sheng-jie Wang, Cai-li Lin, Ming-xing Lin, Wei-wei Song, Qing-hua Tang, Wei Yan, Wei-quan Qin 한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 5호 8 459-467 (9 pages)
Areca palm yellow leaf (AYL) disease caused by the 16SrI group phytoplasma is a serious threat to the development of the Areca palm industry in China. The 16S rRNA gene sequence was utilized to establish a rapid and efficient detection system efficient for the 16SrI-B subgroup AYL phytoplasma in China by loopmediated isothermal amplification (LAMP). The results showed that two sets of LAMP detection primers, 16SrDNA-2 and 16SrDNA-3, were efficient for 16SrIB subgroup AYL phytoplasma in China,... -
Wisteria Vein Mosaic Virus Detected for the First Time in Iran from an Unknown Host by Analysis of Aphid Vectors
Hajar Valouzi, Seyedeh-Shahrzad Hashemi, Stephen J. Wylie, Ali Ahadiyat, Alireza Golnaraghi 한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 1호 8 87-97 (11 pages)
The development of reverse transcription–polymerase chain reaction using degenerate primers against conserved regions of most potyviral genomes enabled sampling of the potyvirome. However, these assays usually involve sampling potential host plants, but identifying infected plants when they are asymptomatic is challenging, and many plants, especially wild ones, contain inhibitors to DNA amplification. We used an alternative approach which utilized aphid vectors and indicator plants to... -
siRNAs Derived from Cymbidium Mosaic Virus and Odontoglossum Ringspot Virus Down-modulated the Expression Levels of Endogenous Genes in Phalaenopsis equestris
Han-hong Lan, Cui-mei Wang, Shuang-shuang Chen, Jian-ying Zheng 한국식물병리학회 The Plant Pathology Journal 13 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 5호 11 508-520 (13 pages)
Interplay between Cymbidium mosaic virus (CymMV)/ Odontoglossum ringspot virus (ORSV) and its host plant Phalaenopsis equestris remain largely unknown, which led to deficiency of effective measures to control disease of P. equestris caused by infecting viruses. In this study, for the first time, we characterized viral small interfering RNAs (vsiRNAs) profiles in P. equestris co-infected with CymMV and ORSV through small RNA sequencing technology. CymMV and ORSV small interfering RNAs (siRNAs)...
