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Roles of Putative Sodium-Hydrogen Antiporter (SHA) Genes in S. coelicolor A3(2) Culture with pH Variation
Kim. Yoon-Jung, Moon. Myung-Hee, Lee. Jae-Sun, Hong. Soon-Kwang, Chang. Yong-Keun 한국미생물생명공학회 Journal of microbiology and biotechnology 9 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2011, Vol.21 No.9 979-987 (9 pages)
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Morphological Variation of Enterobacter sp. BL-2 in Acetate-mediated pH Environment for Excretive Production of Cationic Microbial Polyglucosamine Biopolymer
Son. Mi-Kyung, Hong. Soo-Jung, SaGong. Kuk-Hwa, Lee. Yong-Hyun 한국미생물생명공학회 Journal of microbiology and biotechnology 3 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2008, Vol.18 No.1 104-106 (3 pages)
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Variation in the molecular weight of Photobacterium damselae subsp. piscicida antigens when cultured under different conditions in vitro
Tae S. Jung, Kim D. Thompson, Donatella Volpatti, Marco Galeotti, A. Adams 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 3호 9 255-261 (7 pages)
using sera obtained from sea bass (Dicentrarchus labrax) raised against live or heat-killed Ph. d. subsp. piscicida. The antigenic expression of Ph. d. subsp. piscicida was found to differ depending on the culture medium used. A significantly higher antibody response was obtained with iron-depleted bacteria by ELISA compared with non-iron depleted bacteria obtained from the sera of sea bass raised against live Ph. d. subsp. piscicida. The sera from sea bass raised against live bacteria showed a... -
Effect of pH on the Vascular Tone and 45Ca Uptake in the Aorta of Spontaneously Hypertensive Rats
Chang, Seok-Jong, Jeon, Byeong-Hwa, Kim, Se-Hoon, Kim, Hoe-Suk, Park, Hae-Kun 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 2호 7 169-180 (12 pages)
of extracellular pH (pHo) in the range of 6.5 ~ 8.3 was estimated by comparison with the level of vascular tone at pH 7.4. Contraction was induced below pHo 6.5 in WKY, pHo 7.1 in SHR, and over pHo 8.0 on both strains. The amplitude of contraction induced by high pHo (over pHo 7.7) was similar in SHR and WKY, but that induced by low pHo (below pHo 7.1) in SHR was greater than that in WKY. Either high pHo- or low pHo-induced contractions in WKY and SHR were not induced in the Ca-free Tyrode s... -
Effects of pH, PCO2, and Adenosine on the Contractility of Pig Coronary Artery
Chang, Seok-Jong, Kim, Il-Seon, Jeon, Byeong-Hwa, Kim, Se-Hoon 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1992, 제 26권 제 1호 5 45-54 (10 pages)
Effects of pH, PCO2, and adenosine on the vascular contractility were investigated in the pig coronary arteries. The helical strips of isolated coronary arteries were immersed in the HEPES or HCO3-/CO2-buffered Tyrode s solution equilibrated with 100% O2 or 95% O2-5% CO2 at 35℃. The contraction was recorded isometrically using a force transducer. The amplitudes of contraction induced by ACh, high K+, and electrical Held stimulation (EFS) were decreased by elevating extracellular pH (pHo) and... -
자라에서 본 관류액(灌流液) pH와 심장박출량(心臟搏出量)
양일석(Yang, Il-Suk) 대한생리학회 대한생리학회지 8 Pages
대한생리학회 대한생리학회지 1975, 제 9권 제 1호 9 69-76 (8 pages)
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Genetic Variation and Biological Control of Fusarium graminearum Isolated from Wheat in Assiut-Egypt
한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 2호 8 145-156 (12 pages)
FHB severity. The obtained results indicated that T. harzianum and B. subtilis are very effective biocontrol agents that offer potential benefit in FHB and should be harnessed for further biocontrol applications. The accurate analysis of genetic variation and studies of population structures have significant implications for understanding the genetic traits and disease control programs in wheat. This is the first known report of the distribution and genetic variation of F. graminearum on wheat... -
도림천 상류의 pH, NO3 -, PO4 3-와 식물성 플랑크톤 분포와의 관계
김재근, 김태현, 유지민, 이주연 한국습지학회 한국습지학회지 9 Pages
한국습지학회 한국습지학회지 2012, Vol.14 No.2 10 255-263 (9 pages)
농도 그리고 식물성 플랑크톤을 조사하였다. pH는 5.05 ~ 7.56의 범위에 있었으며, NO3 -와 PO4 3-의 농도는 0.4 ~ 4.9ppm와 0.02 ~ 0.99ppm의 범위에 있었다. 서울대학 교로부터 PO4 3-이 유입되는 것으로 확인되었지만 그 농도는 그리 높지 않았으며, 400m 정도 지나면 거의 모두 정화되었다. NO3 -/PO4 3- 비율은 유입되는 PO4 3-의 영향을 받는 곳을 제외하면 28 ~ 152의 범위로 나타났다. pH, NO3 -, PO4 3-의 농도는 하류로 갈수록 증가하였으며, 이는 한강 본류에서 끌어온 물의 유입뿐만 아니라 비점 오염원으로부터의 유입과... -
몇 종류의 물에서 pH 변화에 의한 유기인계 살충제의 분해과정에 관한 고찰
김종향, 하대식 한국식품위생안전성학회 한국식품위생안전성학회지 5 Pages
한국식품위생안전성학회 한국식품위생안전성학회지 1994, Vol.9 No.4 185-189 (5 pages)
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Differences in Optimal pH and Temperature for Cell Growth and Antibody Production Between Two Chinese Hamster Ovary Clones Derived from the Same Parental Clone
Kim. Sung-Hyun, Lee. Gyun-Min 한국미생물생명공학회 Journal of microbiology and biotechnology 9 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2007, Vol.17 No.5 712-720 (9 pages)
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pH 변화에 따른 N-Nitrosodimethylamine (NDMA) 생성에 대한 고찰
김종오, Kim. Jong-O 대한환경공학회 대한환경공학회지 4 Pages
대한환경공학회 대한환경공학회지 2005, Vol.27 No.4 390-393 (4 pages)
(NDMA)가 미국 및 캐나다 상수도 공급시설에서 발견되어지고 있다. 본 연구에서는, pH가 6, 7, 8인 조건에서 디메칠아민 농도를 0.05 mM로 고정시키고 클로라민 농도(0.001-0.1 mM) 변화에 따른 NDMA 생성 농도를 측정하였다. 디메칠아민 농도에서 NDMA 최대 생성율은 pH 7과 8에서 각각 2.4% 및 1.5%로 나타났으며, 주입 클로라민 농도가 증가할수록 생성율은 크게 증가하는 경향을 보인 것으로 조사되었다. 그리고, pH가 7, 8인 조건에서 NDMA 생성농도와 클로라민/디메칠아민 비율 사이에 높은 연관성을 얻었다($r^2>0.99$). NDMA... -
자연 및 인위적 처리방법 변화에 따른 순환잔골재의 pH저감
한천구, 한민철, 한상윤, Han. Cheon-Goo, Han. Min-Cheol, Han. Sang-Yoon 한국건설순환자원학회 한국건설순환자원학회논문집 8 Pages
한국건설순환자원학회 한국건설순환자원학회논문집 2011, Vol.6 No.1 103-110 (8 pages)
경우 미수화 시멘트의 수화반응을 촉진시켜 수산화칼슘을 용해시키고, 건조과정중 대기중 $CO_2$에 의한 중성화로 pH를 크게 저감시키는 것으로 나타났으며 침수처리에서는 pH 저감 효과가 미흡하였다. $CO_2$촉진의 경우 순환잔골재의 pH저감 효과가 가장 우수한 것으로 나타났는데, 이는 원활한 $CO_2$공급이 가능하기 때문인 것으로 분석된다. 이상의 실험 결과를 종합하면, pH 저감성능, 경제성 및 작업성 측면을 고려할 경우 순환잔골재 질량비 1: 0.5 비율로 살수한 다음, 강제적인 $CO_2$가스의 처리로 건습을 반복한다면... -
Can prosthetic limbs made too quickly cause kidney damage?
Jerrold S. Petrofsky, Mary Browne, Mahyar Jamshidi, Anthony Libo-on, Ha-neul Lee 물리치료재활과학회 Physical Therapy Rehabilitation Science 6 Pages
물리치료재활과학회 Physical Therapy Rehabilitation Science 2014, 제 3권 제 2호 7 119-124 (6 pages)
Objective: The use of chemicals for building prosthetic sockets present the possibility of being hazardous and unsafe due to off-gassing. The purpose of the present study was to investigate if freshly made materials used in prosthetic sockets causes off-gassing that would penetrate the skin and cause damage to the kidneys or blood. Design: Cross-sectional study. Methods: In this research, the off-gassing effects during the initial curing process of styrene monomer, vinyl ester resin, epoxy... -
Variations in lateral abdominal muscle thickness during abdominal drawing-in maneuver in three positions in a young healthy population
Young Jun Ko, Hyun Geun Ha, Ju ri Jeong, Wan Hee Lee 물리치료재활과학회 Physical Therapy Rehabilitation Science 6 Pages
물리치료재활과학회 Physical Therapy Rehabilitation Science 2014, 제 3권 제 2호 4 101-106 (6 pages)
Objective: To investigate the appropriate position for abdominal drawing-in maneuver (ADIM) exercise by rehabilitative ultrasound image. Design: Cross-sectional study. Methods: Twenty-eight young adults with no history of low back pain participated in the study. Three positions compared were crook lying position with hip 60o flexion, standing position with the feet hip width apart and knees straight, and saddle standing positionunsupported with the knees 20° flexed. Once in the appropriate... -
The Current Status of Swine Respiratory Diseases in South Korea: Porcine Respiratory and Reproductive Viruses (PRRSVs) and Porcine Circovirus Type 2 (PCV)
Ga Eun Lee, Jung Ah Kim, Chan Hee Lee, Hee Chun Chung, Hye Jung Yang, Bong Kyun Park 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 53 111-112 (2 pages)
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An enhanced immunochromatographic strip test using colloidal gold nanoparticle-labeled dual-type N proteins for detection of antibodies to PRRS virus
Ji Eun Yu, In-Ohk Ouh, Hyeonjeong Kang, Hye-young Lee, Kwang-Myun Cheong, In-Soo Cho, Sang-Ho Cha 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 4호 7 519-527 (9 pages)
Porcine reproductive and respiratory syndrome (PRRS) is recognized as one of the most important infectious diseases causing serious economic loss in the swine industry worldwide. Due to its increasing genetic diversity, a rapid and accurate diagnosis is critical for PRRS control. The immunochromatographic strip test (ICST) is a rapid and convenient type of immunoassay. In this study, an on-site immunochromatographic assay-based diagnostic method was developed for detection of PRRS virus... -
Comparison of electrophysiological properties of two types of pre-sympathetic neurons intermingled in the hypothalamic paraventricular nucleus
Yiming Shen, Seong Kyu Han, Pan Dong Ryu 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 4호 2 483-491 (9 pages)
The hypothalamic paraventricular nucleus (PVN) contains two types of neurons projecting to either the rostral ventrolateral medulla (PVNRVLM) or the intermediolateral horn (IML) of the spinal cord (PVNIML). These two neuron groups are intermingled in the same subdivisions of the PVN and differentially regulate sympathetic outflow. However, electrophysiological evidence supporting such functional differences is largely lacking. Herein, we compared the electrophysiological properties of these... -
Genomic characterization and pathogenic study of two porcine reproductive and respiratory syndrome viruses with different virulence in Fujian, China
Xiukun Sui, Ting Xin, Xiaoyu Guo, Hong Jia, Ming Li, Xintao Gao, Jing Wu, Yitong Jiang, L.Willems, Hongfei Zhu, Shaohua Hou 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 3 339-349 (11 pages)
Two strains of porcine reproductive and respiratory syndrome virus (PRRSV) were isolated in 2006 and 2016 and designated as FZ06A and FZ16A, respectively. Inoculation experiments showed that FZ06A caused 100% morbidity and 60% mortality, while FZ16A caused 100% morbidity without death. By using genomic sequence and phylogenetic analyses, close relationships between a Chinese highly pathogenic PRRSV strain and the FZ06A and FZ16A strains were observed. Based on the achieved results, multiple... -
Comparative aspects of microRNA expression in canine and human cancers
Kabiru Sahabi, Gayathri T. Selvarajah, Rasedee Abdullah, Yoke Kqueen Cheah, Geok Chin Tan 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 2 162-171 (10 pages)
MicroRNAs (miRNAs) have important roles in all biological pathways in multicellular organisms. Over 1,400 human miRNAs have been identified, and many are conserved among vertebrates and invertebrates. Regulation of miRNA is the most common mode of post-transcriptional gene regulation. The miRNAs that are involved in the initiation and progression of cancers are termed oncomiRs and several of them have been identified in canine and human cancers. Similarly, several miRNAs have been reported to be... -
Polymorphism in the serotonin transporter protein gene in Maltese dogs with degenerative mitral valve disease
Chang-Min Lee, Jae-Ik Han, Min-Hee Kang, Seung-Gon Kim, Hee-Myung Park 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 16 129-135 (7 pages)
Degenerative mitral valve disease (DMVD) is the most commonly acquired cardiac disease in dogs. This study evaluated the relationship between genetic variations in the serotonin transporter (SERT) gene of Maltese dogs and DMVD. Genomic DNA was extracted from blood samples collected from 20 client-owned DMVD Maltese dogs and 10 healthy control dogs, and each exon of the SERT gene was amplified via polymerase chain reaction. The resulting genetic sequences were aligned and analyzed for variations... -
Pharmacokinetics of tilmicosin in healthy pigs and in pigs experimentally infected with Haemophilus parasuis
Ling Zhang, Li Zhao, Yonghong Liu, Junfeng Liu, Xianqiang Li 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 4호 2 431-437 (7 pages)
A comparative in vivo pharmacokinetic (PK) study of tilmicosin (TIL) was conducted in 6 crossbred healthy pigs and 6 crossbred pigs infected with Haemophilus (H.) parasuis following oral administration of a single 40 mg/kg dose. The infected model was established by intranasal inoculation and confirmed by clinical signs, blood biochemistry, and microscopic examinations. Plasma TIL concentrations were determined by a validated high-performance liquid chromatography method with ultraviolet... -
Development of an immunochromatographic strip for detection of antibodies against porcine reproductive and respiratory syndrome virus
Huawei Li, Jifei Yang, Dengke Bao, Jie Hou, Yubao Zhi, Yanyan Yang, Pengchao Ji, Enmin Zhou, Songlin Qiao, Gaiping Zhang 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 7 307-316 (10 pages)
A simple and rapid immunochromatographic test strip incorporating a colloidal gold-labeled recombinant Nsp7 antigen probe was successfully developed for the detection of anti-porcine reproductive and respiratory syndrome virus (PRRSV) antibodies in swine. Recombinant Nsp7 protein of PRRSV labeled with colloidal gold was dispensed on a conjugate pad for use as the detector. Staphylococcal protein A and purified porcine anti-Nsp7 antibodies were blotted on a nitrocellulose membrane to form test... -
Efficacy of horse chestnut leaf extract ALH-L1005 as a matrix metalloproteinase inhibitor in ligature-induced periodontitis in canine model
Se Eun Kim, Tae Hyun Kim, Shin Ae Park, Won Tae Kim, Young Woo Park, Jae Sang Ahn, Manbok Jeong, Min-Young Kim, Kangmoon Seo 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 15 245-251 (7 pages)
Matrix metalloproteinases (MMPs) are the main proteinases associated with periodontal tissue destruction and remodeling. Therefore, inhibition of host-derived MMPs has a key role in the prevention and reduction of periodontitis progression. Horse chestnut (Aesculus hippocastanum L.) extracts have been used as treatments for inflammatory disease, traditionally. This study assessed the clinical effect as a MMP inhibitor of horse chestnut leaf extract ALH-L1005 on periodontitis. ALH-L1005 was... -
Tissue distribution of marbofloxacin in pigs after a single intramuscular injection
Fan Yang, Yiming Liu, Zhili Li, Yuqin Wang, Baobao Liu, Zhensheng Zhao, Bianhua Zhou, Guoyong Wang 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 6 169-173 (5 pages)
Tissue distribution of marbofloxacin was studied in pigs after a single intramuscular injection at 2.5 mg/kg body weight. Samples of plasma, muscle, liver, kidney, heart, lung, and muscle at the injection site were randomly collected from five pigs at 2, 6, 10, 24, 48, 72, and 96 h after administration. Marbofloxacin concentrations were determined by using high-performance liquid chromatography with ultraviolet detection and were subjected to non-compartmental analysis to obtain kinetic... -
Alginate encapsulation preserves the quality and fertilizing ability of Mediterranean Italian water buffalo (Bubalus bubalis) and Holstein Friesian (Bos taurus) spermatozoa after cryopreservation
Sara Perteghella, Alessandro Gaviraghi, Silvia Cenadelli, Valeria Bornaghi, Andrea Galli, Barbara Crivelli, Barbara Vigani, Dani 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 1호 11 81-88 (8 pages)
The use of artificial insemination (AI) in buffalo (Bubalus bubalis) is limited by poor ovarian activity during the hot season, seasonal qualitative patterns in semen, low resistance of sperm cells in the female tract, difficulties in estrus detection, and variable estrus duration. Although AI procedures are commonly used in bovine, use of AI has been limited in buffalo. In the zootechnical field, different studies have been conducted to develop techniques for improvement of fertilizing ability... -
Inflammation affects the viability and plasticity of equine mesenchymal stem cells: possible implications in intra-articular treatments
Laura Barrachina, Ana Rosa Remacha, Antonio Romero, Francisco Jos? V?zquez, Jorge Albareda, Marta Prades, Beatriz Ranera, Pilar 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 1호 6 39-49 (11 pages)
Mesenchymal stem cells (MSCs) are gaining relevance for treating equine joint injuries because of their ability to limit inflammation and stimulate regeneration. Because inflammation activates MSC immunoregulatory function, proinflammatory priming could improve MSC efficacy. However, inflammatory molecules present in synovial fluid or added to the culture medium might have deleterious effects on MSCs. Therefore, this study was conducted to investigate the effects of inflammatory synovial fluid... -
Development and evaluation of an immunochromatographic assay using a gp51 monoclonal antibody for the detection of antibodies against the bovine leukemia virus
Eun-Ju Kim, Kwang-Myun Cheong, Ha-Kyung Joung, Bo-Hye Kim, Jae-Young Song, In-Soo Cho, Kyoung-Ki Lee, Yeun-Kyung Shin 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 6 479-487 (9 pages)
Infection of cattle with bovine leukemia virus (BLV) has been observed and reported worldwide, including in Korea. The onsite identification of infected cattle would help decreasing and eradicating BLV infections on farms. Here, we present a new immunochromatographic assay that employs monoclonal antibodies (MAbs) for the detection of antibodies against BLV in the field. BLV envelope glycoprotein (gp)51 was expressed in E. coli, and MAbs against recombinant BLV gp51 were generated for the... -
Toxicity of crude and detoxified Tityus serrulatus venom in anti-venom-producing sheep
Marina G. Ferreira, Clara G. Duarte, Maira S. Oliveira, Karen L. P. Castro, Ma?lson S. Teixeira, L?lian P. G. Reis, Jos? A. Zamb 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 5 467-477 (11 pages)
Specific anti-venom used to treat scorpion envenomation is usually obtained from horses after hyperimmunization with crude scorpion venom. However, immunized animals often become ill because of the toxic effects of the immunogens used. This study was conducted to evaluate the toxic and immunogenic activities of crude and detoxified Tityus serrulatus (Ts) venom in sheep during the production of anti-scorpionic anti-venom. Sheep were categorized into three groups: G1, control, immunized with... -
Physical-chemical and biological characterization of different preparations of equine chorionic gonadotropin
Rafael Herrera Alvarez, Fabio Luis Nogueira Natal, Maria Teresa Carvalho Pinto Ribela, Beatriz Elane de Almeida, Jo?o Ezequiel d 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 4 459-465 (7 pages)
Ovarian stimulation with commercial preparations of equine chorionic gonadotropin (eCG) produces extremely variable responses in domestic animals, ranging from excessive stimulation to practically no stimulation, when applied on the basis of their declared unitage. This study was conducted to analyze four commercial preparations from different manufacturers via reversed-phase HPLC (RP-HPLC) in comparison with a reference preparation and an official International Standard from the World Health... -
Evaluation of effects of olfactory and auditory stimulation on separation anxiety by salivary cortisol measurement in dogs
Yoon-Joo Shin, Nam-Shik Shin* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 4 153-158 (6 pages)
Separation anxiety (SA) is a serious behavioral problem in dogs. In this study, salivary cortisol was studied to determine if the owner’s odor or voice could reduce SA in dogs. Twenty-eight dogs with SA were divided into three groups: group 1 (control), group 2 (with owner’s clothes during the separation period; SP) and group 3 (a recording of the owner’s voice was played during SP). The dog’s saliva was collected after the owner and their dog were in the experimental room for 5 min (PRE).... -
The association of Hsp90 expression induced by aspirin with anti-stress damage in chicken myocardial cells
Xiao-hui Zhang, Huai-sen Zhu, Zhuang Qian, Shu Tang, Di Wu, Nicole Kemper, Joerg Hartung, En-dong Bao 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 5 35-44 (10 pages)
The protective effect of aspirin during exposure to heat stress in broiler chickens was investigated. We assayed pathological damage, expression and distribution of Hsp90 protein and hsp90 mRNA expression in chicken heart tissues after oral administration of aspirin following exposure to high temperature for varying times. Heat stress induced increases in plasma aspartate aminotransferase, creatine kinase and lactate dehydrogenase activities while causing severe heart damage, which was... -
Magnetic nanoparticle based purification and enzyme-linked immunosorbent assay using monoclonal antibody against enrofloxacin
Nam-Gun Kim, Myeong-Ae Kim, Young-Il Park, Tae-Sung Jung, Seong-Wan Son, ByungJae So, Hwan-Goo Kang 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 6 431-437 (7 pages)
coefficient of variation (CV) of 2.42 to 10.11%. The applicability of the mAb-MNP system was verified by testing the recoveries for ENR residue in three different matrices. Recoveries for ENR ranged from 75.16 to 86.36%, while the CV ranged from 5.08 to 11.53%. Overall, ENR-specific monoclonal antibody was prepared and developed for use in competitive to ELISAs for the detection of ENR in animal meat samples. Furthermore, we suggest that a purification system for ENR using mAb-coupled MNPs could... -
Serum IGFBP4 concentration decreased in dairy heifers towards day 18 of pregnancy
Marie M Meyerholz, Kirsten Mense, Michael Lietzau, Ana Kassens, Matthias Linden, Hendrike Knaack, Elisa Wirthgen, Andreas Hoefli 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 4 413-421 (9 pages)
This study was conducted to determine if the main components of the somatotropic axis change during the early phase of pregnancy in the maternal blood system and whether differences exist on day 18 after pregnancy recognition by the maternal organism. Blood samples of pregnant heifers (Holstein Friesian; n = 10 after embryo transfer) were obtained on the day of ovulation (day 0), as well as on days 7, 14, 16 and 18 and during pregnant, non-pregnant and negative control cycles. The concentrations... -
Preparation and evaluation of enrofloxacin microspheres and tissue distribution in rats
Fan Yang, Jijun Kang, Fang Yang, Zhensheng Zhao, Tao Kong, Zhenling Zeng 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 4 157-164 (8 pages)
New enrofloxacin microspheres were formulated, and their physical properties, lung-targeting ability, and tissue distribution in rats were examined. The microspheres had a regular and round shape. The mean diameter was 10.06 μm, and the diameter of 89.93% of all microspheres ranged from 7.0 μm to 30.0 μm. Tissue distribution of the microspheres was evaluated along with a conventional enrofloxacin preparation after a single intravenous injection (7.5 mg of enrofloxacin/kg bw). The results... -
Concentrations of strontium, barium, cadmium, copper, zinc, manganese, chromium, antimony, selenium, and lead in the liver and kidneys of dogs according to age, gender, and the occurrence of chronic kidney disease
Nadine Paßlack, Barbara Mainzer, Monika Lahrssen-Wiederholt, Helmut Schafft, Richard Palavinskas, Angele Breithaupt, Ju 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 1호 8 57-66 (10 pages)
This study was conducted to measure the concentrations of strontium (Sr), barium (Ba), cadmium (Cd), copper (Cu), zinc (Zn), manganese (Mn), chromium (Cr), antimony (Sb), selenium (Se), and lead (Pb) in canine liver, renal cortex, and renal medulla, and the association of these concentrations with age, gender, and occurrence of chronic kidney disease (CKD). Tissues from 50 dogs were analyzed using inductively coupled plasma mass spectrometry. Cu, Zn, and Mn levels were... -
Evaluation of endoscopically obtained duodenal biopsy samples from cats and dogs in an adapter-modified Ussing chamber
Isabelle Ruhnke*, John V. DeBiasio, Jan S. Suchodolski, Shelley Newman, Mark W. Musch, Jörg M. Steiner 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 17 297-307 (11 pages)
This study was conducted to evaluate an adapter-modified Ussing chamber for assessment of transport physiology in endoscopically obtained duodenal biopsies from healthy cats and dogs, as well as dogs with chronic enteropathies. 17 duodenal biopsies from five cats and 51 duodenal biopsies from 13 dogs were obtained. Samples were transferred into an adapter-modified Ussing chamber and sequentially exposed to various absorbagogues and secretagogues. Overall, 78.6% of duodenal samples obtained from... -
Effect of superovulation on uterine and serum biochemical parameters and its potential association with transferable embryos in Holstein dairy cows
Hasina Santatriniaina Rasolomboahanginjatovo, Younès Chorfi*, Raynald Dupras, Louis Mills, Réjean Lefebvre 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 14 273-281 (9 pages)
control group and serum P4 and PGFM and uterine LDH and PGFM in the SOV group were significantly higher (p ＜ 0.01) at DER than day 7. At DER, uterine urea, LDH, PGFM and TP and serum urea, LDH, PGFM, and P4 concentrations were higher (p ＜ 0.01) in the SOV group than the control. There was no significant variation in uterine bacteriology or cytology. Overall, these results infer that SOV affects both serum profile and uterine secretions, and that these changes may influence the number of TE. -
Extent of Mycobacterium bovis infection in dairy cattle herds subject to partial culling as determined by an interferon-gamma assay
Sungmo Je, Un-chang Yeo, Taeksun Song, Ki-cheol Kim, Sung-Yun Park, Man-Jung Kim, Sang-Nae Cho* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 12 259-265 (7 pages)
The interferon-gamma (IFN-γ) assay is employed as a complementary diagnostic test for bovine tuberculosis (BTB) in many countries. To simplify this assay, we established a 96-well plate format using the ESAT-6 and CFP-10 antigens and then employed it to determine the extent of Mycobacterium (M.) bovis infection in dairy herds with a history of BTB outbreaks in a country where only selective culling is practiced. The sensitivity and specificity of this IFN-γ assay were 85.9% and 100%,... -
Isolation, in vitro propagation, genetic analysis, and immunogenic characterization of an Ehrlichia canis strain from southeastern Brazil
Rosiane Nascimento Alves*, Susana Elisa Rieck, Carlos Ueira-Vieira, Marcelo Bahia Labruna, Marcelo Emílio Beletti 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 10 241-248 (8 pages)
Amplification of the 16S rRNA gene from a blood sample obtained from a dog in southeastern Brazil was used to confirm a naturally acquired Ehrlichia (E.) canis infection. Following isolation and culturing of the new bacterial strain called Uberlândia, partial sequences of the dsb and p28 genes were obtained. The dsb partial sequence of the novel strain was 100% similar to dsb gene sequences of E. canis obtained from different geographic areas around the world. Conversely, the p28... -
Identification of abnormal gene expression in bovine transgenic somatic cell nuclear transfer embryos
Jongki Cho, Sungkeun Kang, Byeong Chun Lee* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 8 225-231 (7 pages)
This study was conducted to investigate the expression of three genes related to early embryonic development in bovine transgenic cloned embryos. To accomplish this, development of bovine transgenic somatic cell nuclear transfer (SCNT) embryos was compared with non-transgenic embryos. Next, mRNA transcription of three specific genes (DNMT1, Hsp 70.1, and Mash2) related to early embryo development in transgenic SCNT embryos was compared between transgenic and non-transgenic SCNTs, parthenogenetic... -
Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus
Lishan Lv, Xiaoming Li, Genmei Liu, Ran Li, Qiliang Liu, Huifang Shen, Wei Wang, Chunyi Xue, Yongchang Cao* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 6 209-216 (8 pages)
Infectious bronchitis virus (IBV) poses a severe threat to the poultry industry and causes heavy economic losses worldwide. Vaccination is the most effective method of preventing infection and controlling the spread of IBV, but currently available inactivated and attenuated virus vaccines have some disadvantages. We developed a chimeric virus-like particle (VLP)-based candidate vaccine for IBV protection. The chimeric VLP was composed of matrix 1 protein from avian influenza H5N1 virus and a... -
An overview of calf diarrhea - infectious etiology, diagnosis, and intervention
Yong-il Cho, Kyoung-Jin Yoon* 대한수의학회 Journal of Veterinary Science 17 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 1 1-17 (17 pages)
Calf diarrhea is a commonly reported disease in young animals, and still a major cause of productivity and economic loss to cattle producers worldwide. In the report of the 2007 National Animal Health Monitoring System for U.S. dairy, half of the deaths among unweaned calves was attributed to diarrhea. Multiple pathogens are known or postulated to cause or contribute to calf diarrhea development. Other factors including both the environment and management practices influence disease severity or... -
Effect of chronic lead intoxication on the distribution and elimination of amoxicillin in goats
Ahmed M. Soliman, Ehab A. Abu-Basha, Salah A. H. Youssef, Aziza M. Amer, Patricia A. Murphy, Catherine C. Hauck, Ronette Gehring 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 4 395-403 (9 pages)
A study of amoxicillin pharmacokinetics was conducted in healthy goats and goats with chronic lead intoxication. The intoxicated goats had increased serum concentrations of liver enzymes (alanine aminotransferase and γ-glutamyl transferase), blood urea nitrogen, and reactivated δ-aminolevulinic acid dehydratase compared to the controls. Following intravenous amoxicillin (10 mg/kg bw) in control and lead-intoxicated goats, elimination half-lives were 4.14 and 1.26 h, respectively. The volumes... -
Preparation and diagnostic utility of a hemagglutination inhibition test antigen derived from the baculovirus-expressed hemagglutinin-neuraminidase protein gene of Newcastle disease virus
Kang-Seuk Choi, Soo-Jeong Kye, Woo-Jin Jeon, Mi-Ja Park, Saeromi Kim, Hee-Jung Seul, Jun-Hun Kwon 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 8 291-297 (7 pages)
A recombinant hemagglutinin-neuraminidase (rHN) protein from Newcastle disease virus (NDV) with hemagglutination (HA) activity was expressed in Spodoptera frugiperda cells using a baculovirus expression system. The rHN protein extracted from infected cells was used as an antigen in a hemagglutination inhibition (HI) test for the detection and titration of NDV-specific antibodies present in chicken sera. The rHN antigen produced high HA titers of 2 per 25 μL, which were similar to those of the...
