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Biological Control of Gom-chwi (Ligularia fischeri) Phytophthora Root Rot with Enterobacter asburiae ObRS-5 to Suppress Zoosporangia Formation and Zoospores Germination
Dayeon Kim, Sang Yeob Lee, Seong Ho Ahn, Ji Hee Han, Jin Woo Park 한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 3호 5 244-254 (11 pages)
Gom-chwi (Ligularia fischeri) is severely infected with Phytophthora drechsleri, the causal organism of Phy- tophthora root rot, an economically important crop disease that needs management throughout the cultiva- tion period. In the present study, Phytophthora root rot was controlled by using bacterial isolates from rhizo- sphere soils collected from various plants and screened for antagonistic activity against P. drechsleri. A total of 172 bacterial strains were isolated, of which, 49 strains... -
Role of Two Sets of RND-Type Multidrug Efflux Pump Transporter Genes, mexAB-oprM and mexEF-oprN, in Virulence of Pseudomonas syringae pv. tabaci 6605
Yuki Ichinose, Takafumi Nishimura, Minori Harada, Ryota Kashiwagi, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Fumiko Taguchi, Daigo Takemoto, Hidenori Matsui 한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 2호 4 148-156 (9 pages)
their sensitivity to plant-derived antimicrobial compounds, antibiotics, and virulence. Growth inhibition assays with KB soft agar plate showed that growth of the wild-type (WT) was inhibited by 5 μl of 1 M catechol and 1 M coumarin but not by other plant-derived potential antimicrobial compounds tested including phytoalexins. The sensitiv- ity to these compounds tended to increase in ∆mexB and ∆mexB∆mexF mutants. The ∆mexB∆mexF mutant was also sensitive to 2 M... -
Wisteria Vein Mosaic Virus Detected for the First Time in Iran from an Unknown Host by Analysis of Aphid Vectors
Hajar Valouzi, Seyedeh-Shahrzad Hashemi, Stephen J. Wylie, Ali Ahadiyat, Alireza Golnaraghi 한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 1호 8 87-97 (11 pages)
The development of reverse transcription–polymerase chain reaction using degenerate primers against conserved regions of most potyviral genomes enabled sampling of the potyvirome. However, these assays usually involve sampling potential host plants, but identifying infected plants when they are asymptomatic is challenging, and many plants, especially wild ones, contain inhibitors to DNA amplification. We used an alternative approach which utilized aphid vectors and indicator plants to... -
Ralstonia solanacearum Type III Effectors with Predicted Nuclear Localization Signal Localize to Various Cell Compartments and Modulate Immune Responses in Nicotiana spp.
Hyelim Jeon, Wanhui Kim, Boyoung Kim, Sookyeong Lee, Jay Jayaraman, Gayoung Jung, Sera Choi, Kee Hoon Sohn, C?cile Segonzac 한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 1호 4 43-53 (11 pages)
Ralstonia solanacearum (Rso) is a causal agent of bacterial wilt in Solanaceae crops worldwide including Republic of Korea. Rso virulence predominantly relies on type III secreted effectors (T3Es). However, only a handful of Rso T3Es have been characterized. In this study, we investigated subcellular localization of and manipulation of plant immunity by 8 Rso T3Es predicted to harbor a nuclear localization signal (NLS). While 2 of these T3Es elicited cell death in both Nicotiana benthamiana and... -
Identification of Differentially Up-regulated Genes in Apple with White Rot Disease
Yeo-Jin Kang, Young Koung Lee, In-Jung Kim 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 5호 13 530-537 (8 pages)
Fuji, a major apple cultivar in Korea, is susceptible to white rot. Apple white rot disease appears on the stem and fruit; the development of which deteriorates fruit quality, resulting in decreases in farmers income. Thus, it is necessary to characterize molecular markers related to apple white rot resistance. In this study, we screened for differentially expressed genes between uninfected apple fruits and those infected with Botryosphaeria dothidea, the fungal pathogen that causes white rot.... -
Characterization of Antibacterial Strains against Kiwifruit Bacterial Canker Pathogen
Min-Jung Kim, Dae-Han Chae, Gyeongjun Cho, Da-Ran Kim, Youn-Sig Kwak 한국식물병리학회 The Plant Pathology Journal 13 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 5호 8 473-485 (13 pages)
Kiwifruit (Actinidia spp.) is an economically important crop and a bacterial canker disease, caused by Pseudomonas syringae pv. actinidiae (Psa), is the most destructive disease in kiwifruit production. Therefore, prevent and control of the disease is a critical issue in kiwifruit industry worldwide. Unfortunately, there is no reliable control methods have been developed. Recently, interest in disease control using microbial agents is growing. However, kiwifruit microbiota and their roles in the... -
Survival Factor Gene FgSvf1 Is Required for Normal Growth and Stress Resistance in Fusarium graminearum
Taiying Li, Boknam Jung, Sook-Young Park, Jungkwan Lee 한국식물병리학회 The Plant Pathology Journal 13 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 5호 1 393-405 (13 pages)
Survival factor 1 (Svf1) is a protein involved in cell survival pathways. In Saccharomyces cerevisiae, Svf1 is required for the diauxic growth shift and survival under stress conditions. In this study, we characterized the role of FgSvf1, the Svf1 homolog in the homothallic ascomycete fungus Fusarium graminearum. In the FgSvf1 deletion mutant, conidial germination was delayed, vegetative growth was reduced, and pathogenicity was completely abolished. Although the FgSvf1 deletion mutant produced... -
vfr, A Global Regulatory Gene, is Required for Pyrrolnitrin but not for Phenazine-1-carboxylic Acid Biosynthesis in Pseudomonas chlororaphis G05
Xia Wu, Xiaoyan Chi, Yanhua Wang, Kailu Zhang, Le Kai, Qiuning He, Jinxiu Tang, Kewen Wang, Longshuo Sun, Xiuying Hao, Weihai Xie, Yihe Ge 한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 4호 7 351-361 (11 pages)
In our previous study, pyrrolnitrin produced in Pseudomonas chlororaphis G05 plays more critical role in suppression of mycelial growth of some fungal pathogens that cause plant diseases in agriculture. Although some regulators for pyrrolnitrin biosynthesis were identified, the pyrrolnitrin regulation pathway was not fully constructed. During our screening novel regulator candidates, we obtained a white conjugant G05W02 while transposon mutagenesis was carried out between a fusion mutant... -
Characterization of Burkholderia glumae Putative Virulence Factor 11 (PVF11) via Yeast Two-Hybrid Interaction and Phenotypic Analysis
Juyun Kim, Namgyu Kim, Mohamed Mannaa, Hyun-Hee Lee, Jong-Seong Jeon, Young-Su Seo 한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 3호 9 280-286 (7 pages)
In this study, PVF11 was selected among 20 candidate pathogenesis-related genes in Burkholderia glumae based on its effect on virulence to rice. PVF11 was found to interact with several plant defense-related WRKY proteins as evidenced through yeast-two hybrid analysis (Y2H). Moreover, PVF11 showed interactions with abiotic and biotic stress response-related rice proteins, as shown by genome-wide Y2H screening employing PVF11 and a cDNA library from B. glumaeinfected rice. To confirm the effect... -
A Duplex PCR Assay for Rapid Detection of Phytophthora nicotianae and Thielaviopsis basicola
Na Liu, Shijun Jiang, Songli Feng, Wenyan Shang, Guozhen Xing, Rui Qiu, Chengjun Li, Shujun Li, Wenming Zheng 한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 2호 9 172-177 (6 pages)
A duplex PCR method was developed for simultaneous detection and identification of tobacco root rot pathogens Phytophthora nicotianae and Thielaviopsis basicola. The specific primers for P. nicotianae were developed based on its internal transcribed spacer (ITS) regions of ribosomal gene, ras gene and hgd gene, while the specific primers for T. basicola were designed based on its ITS regions and β-tubulin gene. The specificity of the primers was determined using isolates of P. nicotianae, T....


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