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Analysis of a Sphingosine 1-phosphate Receptor hS1P3 in Rat Hepatoma Cells
Dong-SoonIm 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 4 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2002, Vol.6 No.3 2 139-142 (4 pages)
To examine intracellular signaling of human S1P3 (hS1P3), a sphingosine 1-phosphate (S1P) receptor in plasma membrane, hS1P3 DNA was transfected into RH7777 rat hepatoma cell line, and the inhibition of forskolin-induced cAMP accumulation and activation of MAP kinases by S1P were tested. In hS1P3 transformants, S1P inhibited forskolin-induced activation of adenylyl cyclase activity by about 80% and activated MAP kinases in dose-dependent and pertussis-toxin (PTX) sensitive manners. In oocytes... -
Nimodipine as a Potential Pharmacological Tool for Characterizing R-Type Calcium Currents
SeogBaeOh 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2001, Vol.5 No.6 8 511-519 (9 pages)
Nimopidine, one of dihydropyridine derivatives, has been widely used to pharmacologically identify L-type Ca currents. In this study, it was tested if nimodipine is a selective blocker for L-type Ca currents in sensory neurons and heterologous system. In mouse dorsal root ganglion neurons (DRG), low concentrations of nimodipine (<10μM), mainly targeting L-type Ca currents, blocked high-voltage-activated calcium channel currents by ∼38%. Interestingly, high concentrations of nimodipine... -
Block of ATP-Sensitive K Channels Expressed in Xenopus Oocytes by Dimethyl Sulfoxide
JinBongParkSooWanChae 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2001, Vol.5 No.2 6 157-163 (7 pages)
The effects of dimethyl sulfoxide (DMSO) were studied in two groups of Xenopus oocytes, one expressing ATP sensitive K (KATP) channel comprised of sulfonylurea receptor SUR1 and inwardly rectifying K channel subunit Kir6.2, and the other expressing renal KATP channel ROMK2. At concentrations of 0.3∼10% (vol/vol) DMSO inhibited whole cell Kir6.2/SUR1 currents elicited by bath application of sodium azide (3 mM) in a concentration-dependent manner. The inhibition constant and Hill coefficient... -
Mechanism of L-NAME-Resistant Endothelium-Dependent Relaxation Induced by Acetylcholine in Rabbit Renal Artery
DongSooYeon, DuckSunAhn, Young-HoLee, SeongChunKwon 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2000, Vol.4 No.6 5 463-469 (7 pages)
In the rabbit renal artery, acetylcholine (ACh, 1 nM∼10μM) induced endothelium-dependent relaxation of arterial rings precontracted with norepinephrine (NE, 1μM) in a dose-dependent manner. NG-nitro- L-arginine (L-NAME, 0.1 mM), an inhibitor of NO synthase, or ODQ (1μM), a soluble guanylate cyclase inhibitor, partially inhibited the ACh-induced endothelium-dependent relaxation. The ACh-induced relaxation was abolished in the presence of 25 mM KCl and L-NAME. The cytochrome P450 inhibitors,... -
Nitroxergic Nerve Relaxes Rat Gastric Smooth Muscle by NO-cGMP Pathway
YoongSamYoon, HyoungChulChoi, YoungSookJung, JongHoKim, KwangYounLee, UyDongSohn, JeoungHeeHa, WonJoonKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2000, Vol.4 No.5 5 369-378 (10 pages)
This study was undertaken to investigate an involvement of nitroxergic innervation in gastric smooth muscle of rat. Isometric tension study, the measurement of single cell length, NADPH diaphorase stain of smooth muscle layers and neuronal nitric oxide synthase (nNOS) western blotting were performed. Sodium nitroprusside (SNP), a nitric oxide donor, relaxed the muscle strips precontracted by acetylcholine (ACh) in a concentration-dependent manner. Pretreatment of L-arginine decreased the... -
The Effects of Sera from Amyotrophic Lateral Sclerosis Patients on Neuromuscular Transmission and Calcium Channels in Mice
HaiDunYan, JimokKim, SungJunJung, JunKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 17 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.1 13 101-117 (17 pages)
Amyotrophic lateral sclerosis (ALS) is a degenerative neuromuscular disease of unknown etiology in which the upper and lower motor neurons are progressively destroyed. Recent evidences support the role of autoimmune mechanisms in the pathogenesis of ALS. This study investigated the effects of sera from ALS patients on neuromuscular transmission in phrenic nerve-hemidiaphragm preparations and on calcium currents of single isolated dorsal root ganglion (DRG) cells in mice. Mice were injected with... -
Ca2+ Signalling in Endothelial Cells Role of Ion Channels
BerndNilius, FelixViana, MasahiroKamouchi, CristinaFasolato, JanEggermont, GuyDroogmans 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 13 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.2 1 133-145 (13 pages)
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Sensory Inputs to Upper Cervical Spinal Neurons Projecting to Midbrain in Cats
JongHoKim, Han-SeongJeong, Jong-SeongPark, Jong-KeunKim, Sah-HoonPark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 11 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.1 2 9-19 (11 pages)
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애착장애 아동에서 99m-Tc-ECD-SPECT로 측정한 뇌혈류량의 양상
천근아, 이경숙, 신의진 대한신경정신의학회 신경정신의학 11 Pages
대한신경정신의학회 신경정신의학 2001, 제 40권 제 1호 10 98-108 (11 pages)
DSM-IV, ICD-10, 낯선상황절차(Strange Situation Procedure), 사회성숙도 검사(Social Maturity Scale), 아동기 자폐증 평가척도(Childhood Autism Rating Scale)가 이용되었다. 진단된 모든 아 동에게 SPECT 촬영이 시행되었으며 두 명의 핵의학 전문의에 의해 판독된 결과에 따라 뇌혈류 양상을 관 찰하였다. 결 과: 12명의 애착장애 아동중 11명이 특정 영역에서 관류(perfusion)가 감소된 비정상적 SPECT 결과를 보였 다. 특히, 10명은 시상(thalamus)에서 감소된 관류(perfusion) 양상을 보였으며, 이중 좌측시상의 관류감소 는...


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