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전체 선택해제
총 787건 검색
본문검색 결과만 보기
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Myocardial Protection by Recombinant Soluble P-selectin Glycoprotein Ligand-1 Suppression of Neutrophil and Platelet Interaction Following Ischemia and Reperfusion
SangSooHam, YoonYoungJang, JinHoSong, HyangMiLee, KwangJoonKim, JunSikHong, YongKyooShin 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2000, Vol.4 No.6 10 507-513 (7 pages)
as PMN accumulation and P-selectin expression were markedly attenuated by a recombinant soluble PSGL-1 which inhibits selectin mediated cell adhesion. These results provide evidence that platelets and PMNs act synergistically in provoking post-reperfusion cardiac dysfunction, and that this may be largely due to cell to cell interactions mediated by P-selectin. These results also demonstrate that a recombinant soluble PSGL-1 reduces myocardial reperfusion injury by platelet and PMNs interaction. -
Bone inducing effect by recombinant human bone morphogenetic protein-2 and collagen carrier in maxillary sinus of the rabbit
Hyo-Jeong Son, Yeong-Wook Kim, Hong-Jun Hwang, Hyoun-Chull Kim, Sang-Chull Lee, Seung-Hwan O, Jingo Kusukawa, Rhuichiro Tanoue 대한치과이식임플란트학회 Journal of Dental Implant Research 8 Pages
대한치과이식임플란트학회 Journal of Dental Implant Research 2011, 제 30권 2호 2 115-122 (8 pages)
Purpose: The aim of this study was to evaluate the bone inducing effects on the maxillary sinus of rabbits using the first commercial rhBMP-2 in South Korea and to investigate the influence of supplementation with collagen sponges. Materials and Methods: Two circular windows (diameter, about 4 mm) was formed in the nasal bone of rabbit The preparated maxillary sinuses were filled with collagen sponges (Terudermis?) alone in three groups. In the other three groups, the preparated maxillary... -
A preliminary study of bone inducing effect by recombinant bone morphogenetic protein-2
Hyo-Jeong Son, Jang-Yeol Lee, Seung-Cheol Woo, Hyoun-Chull Kim, Sang-Chull Lee, Jingo Kusukawa 대한치과이식임플란트학회 Journal of Dental Implant Research 6 Pages
대한치과이식임플란트학회 Journal of Dental Implant Research 2010, 제 29권 1호 6 41-46 (6 pages)
In bone grafting, autogenous bone is considered the 'gold standard' to which other method are compared. The limitations of obtaining autogenous bone are obvious. Allograft, xenofraft and synthetic bone graft are widely used, but they have not solved all the problems in bone grafting. Generally, allograft or synthetic bone graft has lower healing capacity than autograft and carries a risk for certain disease, such as hepatitis or AIDS1). Occasionally, incomplete healing is seen in spite of proper... -
Generation of Antibodies Against Rice stripe virus Proteins Based on Recombinant Proteins and Synthetic Polypeptides
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 1호 7 37-43 (7 pages)
diagnostic detection kit applicable in fields for RSV detection. In this study, RSV proteins that were derived from recombinant proteins and synthetic polypeptides as antigens were generated and were raised in rabbits for antiserum production. Among seven proteins in RSV, genes that code for NCP and NS3 proteins were cloned and subcloned into vector carrying His-tag protein and were expressed in E. coli. Of two recombinant proteins, only anti-NCP displayed stable hybridization signals in western... -
Development of Recombinant Coat Protein Antibody Based IC-RT-PCR and Comparison of its Sensitivity with Other Immunoassays for the Detection of Papaya Ringspot Virus Isolates from India
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 1호 4 25-31 (7 pages)
a ~40 kDa protein. The recombinant coat protein (rCP) fused with 6x His-tag was purified from E.coli using Ni-NTA resin. The antigenicity of the fusion protein was determined by western blot analysis using antibodies raised against purified PRSV. The purified rCP was used as an antigen to produce high titer PRSV specific polyclonal antiserum. The resulting antiserum was used to develop an immunocapture reverse transcription-polymerase chain reaction (IC-RTPCR) assay and compared its sensitivity... -
Evaluation of Toxic Effects Caused by Pesticides in Escherichia coli Using Recombinant Bioluminescent Bacteria
김지원, 구만복, Kim. Jiwon, Gu. Man Bock 한국환경독성학회 환경독성학회지 11 Pages
한국환경독성학회 환경독성학회지 2004, Vol.19 No.3 295-305 (11 pages)
본 연구에서는 유전자 재조합 발광 박테리아를 이용하여 농약에 대한 박테리아의 스트레스 반응과 세포 독성을 분석하였다. 15종류의 농약에 대하여 유전자 손상, 생물막 손상, 산화적 손상 및 단백질 손상을 측정할 수 있는 발광 박테리아와 독성 유무로 인한 세포 독성을 측정할 수 있는 발광 박테리아, 5종을 이용하여 스트레스 반응을 분류하고 세포 독성 정토를 분석하였다. 그 결과, 농약의 화학적 구조가 박테리아의 스트레스 반응에 영향을 미치며, 산화과정이 진행 됨에 따라 독성의 작용 기작이 변하는 것을 확인 할 수 있었다.... -
긴 사슬 지방산 생산을 위해 재조합된 E. coli로부터의 세포 내 지질 추출
함수미, 유인상, 박상준, 김지현, Ham. Su Mi, Yoo. In Sang, Park. Sang Joon, Kim. Ji Hyeon 한국화학공학회 Korean chemical engineering research 5 Pages
한국화학공학회 Korean chemical engineering research 2013, Vol.51 No.4 482-486 (5 pages)
생산하는 재조합 E. coli MG1655를 배양한 후 세포 내 지질을 효과적으로 분리하기 위한 연구를 수행하였다. 고압 균질기를 이용하여 세포를 파쇄한 후 환경친화적인 용매들을 이용하여 지질을 추출하였다. 세포 파쇄는 고압 균질기의 압력이 5,000 psi 보다 큰 압력 하에서 효과적으로 이루어졌으며 20,000 psi 까지의 압력 범위에서는 1~3회 파쇄로 모두 90% 이상의 파쇄율을 얻었다. 추출 용매 시스템의 경우 hexane/isopropyl alcohol과 ethyl acetate/ethanol 시스템이 상대적으로 높은 지질 회수율을 나타내었고 상기 세포 파쇄... -
Expression of a Recombinant Bacillus thuringiensis $delta$-Endotoxin Fused with Enhanced Green Fluorescent Protein in Escherichia coli
Je. Yeon-Ho, Roh. Jong-Yul, Li. Ming-Shun, Chang. Jin-Hee, Shim. Hee-Jin, Jin. Byung-Rae, Boo. Kyung-Saeng 한국잠사학회 International journal of industrial entomology 5 Pages
한국잠사학회 International journal of industrial entomology 2004, Vol.8 No.2 145-149 (5 pages)
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Glycine and Triton X-100 Enhanced Secretion of Recombinant ${alpha}$-CGTase Mediated by OmpA Signal Peptide in Escherichia coli
Li. Bin, Wang. Lei, Su. Lingqia, Chen. Sheng, Li. Zhaofeng, Chen. Jian, Wu. Jing 한국생물공학회 Biotechnology and bioprocess engineering 7 Pages
한국생물공학회 Biotechnology and bioprocess engineering 2012, Vol.17 No.6 1128-1134 (7 pages)
