Cytological observations were carried out to investigate the frequencies and geographical distribution of accessory chromosomes in rye in Korea. (1) All the 41 strains of rye investigated were found to have accessory chromosomes and the frequencies of accessories are ranged from 2 to 73.3%. (2) The frequency ranging from 30 to 40% was predominant and it included 13 different strains. (3) The edaphic factor seemed to play more marked role than the climatic factor in determining geographical distribution of accessories in rye in Korea. (4) Breakages of A-chromosomes into two fragments were observed in 11 plants. This phenomenon may throw some light on possible orgin of accessory chromosomes in rye.