Some considerations were made concerning the variation in the number of vertebrae (except urostyle) of anchovies sampled from the south coast of Korea, mainly off the city of Chungmu. The some seasonal difference was evinced in the mean value of vertebrae of small sized fish only, and no other significant differences were observed such as for locality or fishing gears. It was concluded that the anchovies from the south coast of Korea might be devided into spring and summer hatching populations and that the seasonal variation in the number of vertebrae might be caused by the difference of water temperature during the spawning season.