Results obtained from the observation of 1,000 matured resting conidia of Pyricularia oryzae CAV. were as fellows. The percentage of a condium conitaining was 95.5, while that of a multinucleate conidium was 4.5. Formation of the three-celled mature conidium containing a nucleus in each cell, which was derived from the immatured conidium, could be recognized. Chromosomes could be observed in the conidium when the nuclear division took place. The number of chromosomes was n=3, 4, 5 or 6 but rile majority was 5. The nucleus was moved into the germ-tube from the conidium by following ways: (a) a resting nucleus divided into two nuclei in the conidium and one of them was moved into the germ-tube. The other nucleus remained in the conidium (43 per cent): (b) a nucleus was moved into the germ-tube from the conidium without nuclear division (57 Per cent). The appressorium without a nucleus was abundantly observed when stain was made. However, tile number of a mononucleate appressorium was 476 out of 500 appressoria which had nuclear sap. On the ground of this experiment, we could support the conidium of blast fungus contained mononucleus and also homokaryon seemed to b: appealed on the conidia even though those were multilocular. As the results, it could be concluded that use of inoculum derived from single spore isolate was reasonable for any experiments in the rice blast fungus.