Nutritional anemias are an important nutritional problem affecting large population groups in most developing countries. Many reports on investigations of nutritional anemias have been published and there is good evidence that prevalence of iron deficiency anemias in vulnerable groups is high in many areas of the world. However, there is a general lack of accurate data on its prevalence throughout the world. Methods used to measure different factors have been variable and often of poor quality. Furthermore recent comparison of values obtained in different laboratories have shown a much greater discrepancy in iron binding capacity measurements as compared with serum iron determinations. Since the major cause of these differences appears related to the technique employed, the efforts of the central laboratory will be directed initially to standardization of methodology. My laboratory has been joined interlaboratory comparison of WHO studies of iron standardization. The determination of serum iron, iron-binding capacity and transferrin saturation was done on healthy adults, 79 males and 20 females, who visited hospital for health certificate from January to July 1970. The serum iron was determined by the method of modification of Bothwell and Mallett and total iron blinding capacity by Ramsay method and Bothwell and Mallett method. The results of this study are as follows; 1) The serum iron concentration of seventy nine adult male by the method presented is $131.5{pm}37.3{mu}g/100ml;(range;52.5{sim}225.0{mu}g/100ml)$ and of twenty female adult is $108.5{pm}40.2{mu}g/100ml;(range;45.0{sim}202.5{mu}g/100ml)$. 2) The adult male level of serum iron-binding capacity is $330.9{pm}48.9{mu}g/100ml;and;adult;female;is;291.2{pm}55.2{mu}g/100ml$. 3) The transferring saturation of healthy male is $35.6{pm}12.8%;(range;15.1{sim}18.0%);and;of;female;as;25.4{pm}12.4%;(range;10.1{sim}60.0)$.