A new physico-chemical method for identifying botanical drugs was investigated. Fifty drugs were chosen from the presently exported botanical drugs and were classified into 12 groups chemotaxonomically. Various extract(either, 50% ethanol and water) of each group were developed with same solvent system by using chromatographic method(TLC) and observed a characteristic pattern of each drugs. This method, therefore, can be applicable to identify each botanical drug out of the combined phyto-preparations.