This paper was studied on the behavior, crack formation and propagation of slip bands on low carbon steel which was heat-treated in three conditions in order to change metallic structure. The specimens were tested by rotating bending fatigue testing machine and also observed the variations of grains by microscope. From the test results it was clear that fatigue endurance limit and life of low carbon steel were more increased in contrast with the case that the grain size of specimen was more decreased. Slip bands developed at oil-quenched specimen and furnace-cooled specimen. Formed cracks in the first one or two grains below the surface were approximately "planar" type, there after they followed "wavy" type. It was also found that cracks at 30% higher stress than fatigue limit were usually developed inter-granular, and cracks at 12% higher stress than fatigue limit were propagated meandering path, partly trans- and partly inter-grandular.