To find the varietal differences of the protein accumulation in rice kernel. three rice varieties were planted under the conditions of different planting dates and different nitrogen levels. Samples of leaf blade, culm and rice grain were obtained at 10 day intervals during grain development period, and analyzed by the kjeldahl method for nitrogen content. The results are summarized as follows; 1. The nitrogen contents in leaf and culm were decreased as the grain development progresses. Varietal differences were found in the change of the nitrogen contents in leaf and culm. IR lines, high protein lines, reduced rapidly as the grain development progresses, but Jinheung, a leading variety in central region of Korea, showed slow decline. 2. Heavy nitrogen fertilizer application increased the nitrogen contents in the leaf and culm. Varietal differences were also found. IR lines showed higher increase than the Jinheung. 3. The protein content in brown rice were decreased as the ripening progresses to the maturity. Varietal differences were noticed in the declining trend of the protein content in brown rice. IR lines showed less changes than Jinheung and finally Jinheung showed low protein content than IR lines at maturity stage. 4. Increased nitrogen fortilizer application raised strikingly the protein content of brown rice in the IR lines but much less in the Jinheung.