Liver scanning with radioactive colloids has been widely used as an important method for the diagnosis and localization of intrahepatic lesions such as hepatoma, abscess and cyst. Such lesions are seen on the scan as filling defects, however, this information is nonspecific. Since 1970, Gordon and Cuaron have been using a combined method of liver scanning with radiocolloid first and later with transferrin-bound 113m In and have obtained improved differential diagnosis between tumors and other filling defects. As a preliminary study, 5 patients with primary hepatoma and 5 patients with amebic abscess were studied in an attemept to improve differential diagnosis of the two. A liver scan was performed with colloidal 113m In; a few hours later or on the next day, a second scan was obtained with transferrin-bound 113m In to visualize the vascular bed of liver. In all cases the first scan showed one or more filling defects. The second scan demonstrated increased radioactivity inside the lesion in 3 cases of hepatoma, whereas the increased radioactivity was noted in the periphery of cold area in 4 cases of amebic abscess. As Grodon and Cuaron have shown the combined method of liver scanning using transferrin-bound 113m In and radiocolloid seems to give increased differential diagnosis of hepatoma and amebic abscess.