This experiment was carried out to obtain a marked larvae in sex-limited inheritance, using F$_1$ hybrid Bo ok X Chun san introduced from Japan. Sequence of backcrossing has been done through the earlier generation with a recessive character of plain marked larvae. 1. It is noted that genotypic segregation of sex-limited larval marking was observed in F$_4$ generation; female possesses larval marking and male shows a plain marking. 2. Larval marking heredity follows a diagram of segregation with an expected genotype such as ♀ ： (W$.$＋p)/Z$.$P/P and ♂ ： Z/Z$.$P/P. 3. It is observed that dissociation was occurred to produce female with a genotype of W/Z$.$P/P in segregation ana selection. 4. Abnormal ratio of sexuality is observed in the course of segregation and segregation and analysis of it is continuously under way. 5, It is observed that the difference of qualitative characteristics between female and male obtained from the original variety shows the same tendency as the normal marked variety.