Spinous loaches widely distributed in Korea, are of many color pattern types, but interestingly the crossband B and C-types of these coaches are collected only in the two rivers, the Yeongsan River and the Seomjin River, flowing through the south-western part of Korea. These two types have not only different color pattern, but also different geographical distribution: the B-type is found in the Yeongsan River and the C-typs, in the Seomjin River. But the both types have four common distinctive features, which easily distinguish them from other already known species of this genus: the B and C-types have the similar total length distribution, the same structure of lamina circularis and scales, and the black spots at the back of their opercula. These peculiarities may be explained by studing the history of these two rivers. The author considers that they are once rivers of the same origin located around the Noryeong and the Sobaeg Mountains, and then were eventually separated from each other as the water level or the crust of Korea peninsula changed. Thus, these two types, B and C, of spinous loaches should have been the same species living in the same river, but in the course of time, these two types have taken different color patterns since the separation of two rivers thus making them impossible to cross between these two populations.