주유소 공장주변의 하수 및 토양으로부터 Methanol 자화성 효모 7주를 분리하고 그중 생장이 완성한 균주 2주에 대하여 분류학적 제성질을 검사한 결과 양주는 모두 Candida속으로 동정되었다. 양주는 모두 ethanol 자화성을 가지고 생육에는 Biotin을 요구하였으며 배양특성은 최적온도 28$^{circ}C$, 최적 pH5.0이고 methanol 농도 1%에서 중식이 균체수율은 대소비 methanol에 대하여 35~40%에 달하였다.
Seven strains of methanol assimilating yeasts were isolated from soil enriched with tetracycline. Among them two better growing strains were selected and partially identified as species belonging to genus Candida. The both Candida 1-B and 25-A, grew best under conditions of pH 5.0 and 28$^{circ}C$. The optimal methol concentration in the medium was found to be 1％, Whereas the organism, could grow up to the 4％ level. Biotin was required by the organisms for growth and organic nitrogen sources raised the rate of growth. The field of biomass per unit weight of consumed methanol after 96 hours were 36.9％ by Candida 1-B and 39.2％ by 25-A.
