The root-out of mulberry saplings has been studied by pour into soil and spray on leaves of the plant growth substances. In addition, the effect of "Rutin", a kind of plant growth hormones has been also studied on promoting the rootability of mulberry scions by dipping the scions at the various concentrations. The results are summarized as follows: (1) It is likely to be effective on acceleration of the rootability resulting in increase of rooting ratio by pour into soil at the concentration of 1 ppm and 10 ppm NAA, 10 ppm and 100 ppm Rutin, and 5000 fold solution of Atonic, respectively (2) Growth of branch is only promoted by leaf spray of 0.5% urea, 0.005% and 0.01% Rutin, and 5000 fold solution of Atonic without increasing the root weight. (3) It seems that 0.05% of Rutin is of practical use, and 0.05% to 0.4% of "Rutin" accelerates the root-out of mulberry scions as well as NAA does.