한국산 인삼뿌리의 한성분인 인삼사포닌이 정상인의 혈청 (Moni-trol, Dada) Glutamate-pyruvate Transaminase (GPT, EC 2.6.1.2)에 미치는 영향을 관찰하였다. 효소반응액에서의 인삼사포닌의 농도가 $1{ imes}10^{-7}%$ 일때 GPT 활성이 가장 활발하였으나 사포닌의 농도가 그이상으로 증가되면 사포닌의 효소활성화율은 저하되고 $10^{-2}%$ 일때는 오히려 효소활성이 억제된다. L-alamine에 대한 GPT의 Km을 조사한 결과 $1{ imes}10^{-7}%$의 사포닌 존재하에서의 Km(0.27mM)는 대조군의 Km(0.65mM)의 불과 1/3이 지나지 않았으며 사포닌에 의한 효소활성은 Km 값의 저하에 기인되는 것으로 풀이된다.
The activity of GPT (Glutamate-pyruvate transaminase, EC 2.6.1.2) of normal human blood serum (Monitrol I, Dade) in the presence of ginseng saponin, one of the components of Korean panax ginseng (C.A. Meyer) root, was estimated. It was found that the highest GPT activity was observed when the saponin concentration in the assay mixture was $1{ imes}10^{-7}%$. When the saponin concentration was over $1{ imes}10^{-7}%$, however, the GPT stimulation rate was lowered suggesting that there is an optimal concentration of the saponin for the maximum GPT activity. Km of GPT for L-alanine in the presence of various amounts of the saponin was determined and was found that K_m(0.27mM) in the presence of $1{ imes}10^{-7}%$ of saponin was greatly lower than that (0.65mM) of control. This suggests that the stimulation effect of moderate amounts of the saponin might be brought by lowering Km value.
