"Ha-Neul-Ta-Ri" (Trichosanthes kirilowii Max., Cucurbitaceae) cultivated in Korea and used in popular medicine, was investigated for sterol composition of the seed oil. This seed oil contains unusual sterols having ${delta}^{7, 22}_-$, ${delta}^{7, 24}_-$ and ${delta}^{7, 22, 25}_-$ di and trienols. ${delta}^{7, 22}_-$ Stigmastadienol and ${delta}^{7, 22, 25}_-$ Stigmastatrienol were identified as the major sterols in the seed oil of Trichosanthes kirilowii by gas loiquid chromatographic analysis. The results showed that contents of the seed sterols were campesterol 1.0-1.2%, stigmasterol 1.5-1.7%, ${delta}^{7}_-$ campstenol 0.6-0.8%, sitosterol 10.6-11.4%, ${delta}^{7, 22}_-$ stigmastadienol 39.1-41.8%, ${delta}^{7, 22, 25}_-$ stigmastatrienol 28.9-29.3%, ${delta}^{7, 25}_-$ stigmastadienol 14.9-15.5% and ${delta}^{7, 24}_-$ stigmastadienol 0.7-1.0%.