In general, the lock structure is built at place of having great deal range for dealing with effective ships operation such as in the west coast of Korea. In Inchon harbour, the two locks of 10 KT and 50 KT tonnage class in total has been constructed for several years age, however, it has been recorded many waiting vessels at outer harbour due to the increment of oceangoing vessels & shortage of berthing facilities in accordance with beyond expectation of cargo amount increment. This paper attempts to solves the waiting vessels problem at outer harbour by simulation in applying queing theory. It is found that the simulation results such as average queue time, service time, and queue length during lock operation can be applied to find the minimum of the cost function for determination of optimum Lock Size