Fifty two human mandibular second molars were chosen to study the anatomy of the root canal. The experimental teeth were injected with china ink, decalcified, cleared and used in study, in vitro, to determine the number of root, the number of root canals, canals per root, frequency and location of transverse anastomoses, frequency and location of lateral canals and frequency of the apical deltas. The results were as follows; 1. Most of the teeth showed three canals, but 19.2% of the teeth were found to have two canals and 9.6% of them four canals. 2. In so far as observing two canals per root, 80.8% of the teeth were found to have two canals in mesial root and 9.6% of them in distal canal. 3. In roots with, two canals, the seperated apical foramen appeared in 59.5% in mesial side and 40.0% in distal side, and the common apical foramen appeared in 40.5% in mesial side and 60.0% in distal side. 4. Of the two root canals in one root, 36.2% of the canals were found to have transverse anastomoses and were usually located in the apical third of the root. 5. 23.1% of 52 teeth were found to have lateral canals, and ramifications were mainly located in the apical third of the root.