Paeonol-glycoside could be extracted and isolated from the fresh roots of Pycnostelma paniculatum Kitagawa and compared with paeonolide(paeonol-6-[L-arabinosyl]-${eta}-D-glucoside)$ by IR and UV spectra. By saponification with mineral acid, it was known that isolated glycoside was composed of aglycon and sugar parts. Aglycon was identified by comparing with paeonol by TLC and UV spectra. Of two kinds of sugar, only glucose was identified by GLC.