합성배지(合成培地)에서 고온성양송이 (Agaricus bitorquis)와 느타리 (Pleurotus ostreatus)의 균사생육(菌絲生育)을 실험(實驗)한 결과(結果)를 요약하면 다음과 같다. 1. 균사생육(菌絲生育)의 최적(最適) pH와 온도(溫度)는 A. bitorquis경우 pH $6.0{sim}6.5$, 온도(溫度)는 $25{sim}30^{circ}C$, P. ostreatus는 pH $5.0{sim}6.5$,온도(溫度)는 $25^{circ}C$이었다. 2. 균사생육(菌絲生育)이 양호(良好)한 탄소원(炭素源)과 질소원(室素源)은 A. bitorquis 경우 glucose, starch와 peptone이었고, P. ostreatus 는 glucose, fructose, starch와 peptone이었다. 3. C/N률(率)이 너무 낮거나 높으면 오히려 균사수율(菌絲收率)이 감소(減少)하였고 동일 C/N률(率)에서는 glucose와 peptone의 농도(濃度)가 높은 편이 균사수율(菌絲收率)이 높았다. 4. 균사생육(菌絲生育)이 양호(良好)한 vitamin은 A. bitorquis 경우 thianmine, Ca-pantothenate, folic acid이었고 P. ostreatus는 thiamine, folic acid, inositol이었다. 5. 배지(培地)의 성분(成分)은 배양기간(培養期間)의 경과에 따라 pH, 총질소(總窒素) glucose는 점진적으로 감소되었고 균사수율(菌絲收率)은 이와 반대로 증가 되었다.
The mycelial growth of Agaricus bitorquis and Pleurotus ostreatus in synthetic media were carried out by ordinary methods. The optimum pH and temperature for mycelial growth were from pH 6.0 to 6.5 and 25 to $30^{circ}C$, and from pH 5.0 to 6.5 and $25^{circ}C$ in A. bitorquis and P. ostreatus, respectively. Among the carbon and nitrogen sources, glucose, starch, and peptone showed the good result for the mycelial growth of A. bitorquis, and glucose, fructose, starch and peptone were good for the mycelial growth of P. ostreatus. The yield of mycelium decreased under lower or higher C/N ratio. Also, at the same C/N ratio, the higher the concentration of glucose and peptone, the more the yield was increased. Among various vitamins thiamine, Ca-pantothenate and folic acid were suitable for the mycelial growth of A. bitorquis, and thiamine, folic acid and inositol for the mycelial growth of P. ostreatus. Although pH, total nitrogen and glucose contents of media decreased gradually during culture period the yield of mycelium increased.
