Aspergillus niger가 분비하는 섬유소분해효소(纖維素分解酵素)를 Sephadex G-150 column을 통과시켜서 ${eta}-glucosidase$와 CM-cellulase을 분리(分離)하고 CM-cellulase는 다시 DEAE-Sephadex A-50 column을 통과시켜서 세개의 CM-cellulase F I, F II , F III를 분리하여 그 특성(特性)을 각기 조사 하였다. F I의 최적조건(最適條件)은 pH 4.0 및 $50^{circ}C$였고 열불활성화특성(熱不活性化特性)은 Arrhenius plot에서 2개의 기울기가 다른 직선을 나타냈다. 즉 $60{sim}70^{circ}C$ 사이에서는 Z값이 $4^{circ}C$ 이고 $70{sim}98^{circ}C$ 사이에서는 $383^{circ}C$였다. F II는 pH 4.7 및 $50^{circ}C$에서 최적조건(最適條件)을 보였고 Z값은 $8^{circ}C$, F III는 pH 4.3 및 $60^{circ}C$에서 높은 활성(活性)을 가졌고 Z값은 $10^{circ}C$였다. 조(粗) CM-cellulase의 경우는 최적조건(最適條件)이 pH 4.3 및 $60^{circ}C$로 F I , F II 및 F III와 비슷한 경향을 보였으나 Z값은 $21.5^{circ}C$로 큰 차이(差異)를 보였다. 한펀 ${eta}-glucosidase$는 정제효소(精製酵素) 및 조효소(粗酵素)가 다 같이 pH 4.7 및 $60^{circ}C$에서 가장 높은 활성(活性)을 나타냈으며 Z값은 $7^{circ}C$였다.
Three fractions of carboxymethyl-cellulase (F-I, F-II, and F-III) and ${eta}-glucosidase$ form Aspergillus niger were partially purified by ammonium sulfate fractionation. Sephadex G-150 and DEAE-Sephadex column chromatography. The optimum conditions such as pH and temperature and thermal inactivation properties of the enzymes were investigated. Arrhenius plots of F-II and F-III appeared as straight lines, whereas that of F-I was biphasic. The Z-values of F-II and F-III were $8^{circ}C$ and $10^{circ}C$ respectively, while that of F-I was $4^{circ}C$ over $60{sim}70^{circ}C$ and $383^{circ}C$ over $70{sim}98^{circ}C$. Three fractions and the crude extract of carboxymethyl-cellulase exhibited a similar optimum pH 4.3 and temperature of $60^{circ}C$, while Z-value of crude extract $(21.5^{circ}C)$ was much higher than that of the purified enzyme. Maximum activity of both purified and crude extract of ${eta}-glucosidase$ was shown at pH 4.7 and $60^{circ}C$, and z-value of the enzyme was $7^{circ}C$.
