The lipid composition of total serum lipids were investigated by latroscan TH-10 TLC analyzer in 69 healthy subjects and 62 patients with hypercholesterolemia. Total serum lipids level in healthy subjects was higher in females than males. There was a increase in total serum lipids level with increasing total serum cholesterol level in patients with hypercholesterolemia, regardless sex. Total serum lipids obtained from healthy subjects and patients with hypercholesterolemia were separated into phospholipids, free cholesterol, free fatty acids, triglycerides and cholesterol esters. Increasing the level of total serum cholesterol in the patients resulted in a decrease in the level of phospholipids, which was a major lipid fraction, indicating that HDL level in the patients was also decreased. However, cholesterol esters, triglycerides and free cholesterol levels in the patients were higher than those found in healthy subjects. The FC/CE ratio for male patients was lower than for healthy males, while in female patients, this ratio was similar to that observed in healthy females.