- 인삼근 및 자엽 Callus의 기관분화에 관한 연구
- ㆍ 저자명
- 최광태,김명원,신희석,Choe. Gwang-Tae,Kim. Myeong-Won,Sin. Hui-Seok
- ㆍ 간행물명
- 고려인삼학회지
- ㆍ 권/호정보
- 1981년|5권 1호|pp.35-40 (6 pages)
- ㆍ 발행정보
- 고려인삼학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
Explants of mature root tissues and calli derived from root and cotyledon of Panax ginseng were cultured in vitro on Murashige and Skoog medium supplemented with 2, 4-dichlorophen-oxyacetic acid(3,4-D), naphthaleneacetic acid(NAA), benzyladenine, and gibberellic acid to assess their capacity to regenerate organs. Root formation at high percentage (46.2-61.1%) was obtained 20-30 days after culturing on media supplemented with combinations of NAA(5 mg/l) and kinetin (1 mg/l), And calli derived from cotyledon produced numerous embryoids in media($frac{1}{2}$MS) containing 2,4-D(0.5 mg/l) and kinetin (0.5 mg/l). Reculture of these embryoids in media($frac{1}{2}$MS) enriched with 1 mg/l of benzyladenine and 1 mg/l of gibberellic acid resulted in more plantlet regeneration.