Psychrophilic yeast strains, HJ3011, HJ3023, and HJ3021, which were isolated from the Han River during winter season of December 1980 to February 1981 were identified as Candida sp., Candida nivalis, and Rhodotorula rubra respectively. The effect of temperature on growth was carried out in this study. Growth rate constants of strains HJ3011 and HJ3023 were the highest value at $15^{circ}C;and;16{sim}20^{circ}C$ respectively, and both growth rates diminished gradually above $20^{circ}C$ and were decreased to zero at $25^{circ}C$. According to the results obtained in this experiment, above two strains of yeast were confirmed as psychrophile. On the other hand since the strain of HJ3021 exhibited the highest growth rate constant at $15^{circ}C$ and could slightly grow above $25^{circ}C$, this strain was defined as psychrotroph.