Water Pollution of Gwangju Stream was examined for four months from 22 March 1981 to 7 June, 1981. For checking the water pollution, 6 sampling positions were selected 5 from main stream. We assumed that the water pollution of Gwangju stream was due to the organic materials, which came from the domestic sewage. The apparatus for this test were pH meter (Orion model 301), Dissolved Oxygen & Temperature meter(Delta model 1010). The results we obtained are as follows: 1. The average range for mid-stream(sp. 3, 4) was 3. 10~2.73ppm of Dissolved Oxygen(DO), 80.23~102.95ppm of Biochemical Oxygen Demand (BOD), 195. 10~165.90ppm of Suspended Solid (SS), 6.6~6.7 of pH. 2. The plankton identification in this survey period showed Cyanophyceae is 4 genera 5 species, Bacillariophycea 11 genera 14 species, Chlorophyceae 8 genera 15 species and Zooplankton 6 genera 7 species: total 29 genera 41 species. 3. To appear dominant plankton in polluted water, there were Paramecium, Euglena and Oscillatoria. 4. The results of biological water analysis were as follows: st. 1 was B-mesosaprobic to oligosaprobic, but st. 4 and st. 5 at stream in the midtown area seemed to be polysaprobic.