Proteins, which may be closely related to differentiation of a cell group into a predestined fate, were investigated using imaginal discs of a wing mutant, vestigial of Drosophila melanogaster. The wing discs of the mutant fail to differentiate into normal wings, even though the third instar larvae form wing discs, which are very similar to those of the normal strain in size and shape. Patterns of proteins of accumulated or synthesized in the discs of the third instar larvae of the normal and mutant strains were analyzed by acrylamide gel electrophoresis. The patterns of accumulated proteins were found to be slightly different between two strains in quantity rather than in quality. The patterns of proteins synthesized at various times of the third instar were found to be very similar to each other, even though there were a few proteins specific to the normal or to the mutant strain.